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Qu J.,South China University of Technology | Liu L.,South China University of Technology | Chen H.,South China University of Technology | Ogata N.,Ogata Research Laboratory Ltd | Masuda T.,Fukui University of Technology
Macromolecular Chemistry and Physics | Year: 2010

A novel DNA-lipid complex carrying carbazole (Cz) moieties was prepared by substituting the sodium counter cation with cationic lipid, namely lipid(2Cz), in which the actual mole ratio of phosphate to lipid was 1:1.05. The DNA-lipid(2Cz) complex was soluble in common organic solvents including CHCl3, CH2Cl2, methanol, and ethanol, while insoluble in THF, toluene, and water. CD spectroscopy revealed that the DNA- lipid complex took a predominantly double helical structure in methanol and that the helical structure was fairly stable against heating. A solution of the DNA-lipid(2Cz) complex emitted fluorescence in 40.0% quantum yield, which was lower than that of the corresponding lipid(2Cz) (76%). The cyclic voltammograms of the complex indicated that the oxidation potential of DNA- lipid(2Cz) was 0.65 V. The onset temperature of weight loss of the DNA-lipid complex is 230 °C according to TGA in air. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Ogata N.,Ogata Research Laboratory Ltd
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2011

The large enhancements of optical properties of the dye-intercalated DNA lead us to apply the dye-intercalated DNA as various sensors with a high sensitivity to detect environmentally toxic gases such as dioxine, NOx or carbon monoxide. This paper retorts on DNA sensors for the further applications of DNA as materials. Also, bio-medical applications of DNA sensors such as a glucose sensor are reported. © 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).


Ogata N.,Ogata Research Laboratory Ltd
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2012

This paper describes preparations of nano-scale patterned electric circuit based on high purity DNA molecules which are obtained from Salmon roe. The patterning on silicon substrate was carried out by an ink-jet method of aqueous solution of DNA. However, the Salmon-based DNA has a so huge molecular weight of over billions that even only 1wt% aqueous solution of DNA becomes gel without any fluidity. So, it is necessary to reduce molecular weights of DNA to increase fluidity of the aqueous solution of DNA, keeping the characteristic feature of double helical structures of DNA molecules to form metal-chlating complexes with various metal cations such as silver or copper cations. Several methods to reduce the molecular weight of DNA, including hydrolytic , enzymatic degradations and ultra-sonification. It was found that the best method to reduce the molecular weights (MW) of DNA was an enzymatic degradation of DNA to increase fluidity, thus being able to apply an ink-jet method for nano-scale patterning on a silicon wafer to form DNA circuit, followed by ion-crosslinking of DNA by dipping the patterned DNA circuit into aqueous solution of copper chloride. The DNA-CuCl2 patterned circuit was reduced to copper nano-lines for electric circuit, by using hydrazine as a reducing agent. Thus, all DNA devices can be prepared by the combination of DNA transistor and circuit. © 2012 SPIE.


Zhang T.,South China University of Technology | Qu J.,South China University of Technology | Ogata N.,Ogata Research Laboratory Ltd | Masuda T.,Fukui University of Technology
European Polymer Journal | Year: 2011

Novel DNA-lipid complexes carrying pyrene and anthracene were prepared by substituting sodium counter cations with cationic amphiphilic lipids, namely lipid(PY) and lipid(Anth), in which the actual mole ratios of phosphate to lipid were 1:1.11 and 1:1.03, respectively. DNA-lipid(PY) and DNA-lipid(Anth) complexes were soluble in common organic solvents including CHCl3, CH2Cl2, methanol and ethanol, while insoluble in THF, toluene, and aqueous solutions. CD spectroscopy revealed that DNA-lipid(PY) and DNA-lipid(Anth) complexes took a predominantly double helical structure in methanol and that the helical structure was fairly stable against heating. The solution of DNA-lipid(PY) complex emitted fluorescence in 27.8% quantum yield, which were higher than that of the corresponding lipid(PY) (16.8%), while the fluorescence quantum yields of the solution of DNA-lipid(Anth) (45.4%) was lower than that of the lipid(Anth) (53.0%). The onset temperatures of weight loss of DNA-lipid(PY) and DNA-lipid(Anth) were both 220 °C according to TGA in air. © 2011 Elsevier Ltd. All rights reserved.


Ogata N.,Ogata Research Laboratory Ltd | Yamaoka K.,Ogata Research Laboratory Ltd | Yoshida J.,Chitose Institute of Science and Technology
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2010

Crosslinking reactions of DNA film by UV irradiation were investigated in terms of structural changes which indicated the formation of -O-P-O- bond. The UV-cured DNA films were applied to medical uses for cell culture and wound-healing of skin, which were very effective for medical applications. © 2010 SPIE.

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