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Koppel R.,Official Food Control Authority of the Canton | Kuslyte A.R.,Official Food Control Authority of the Canton | Tolido I.,Official Food Control Authority of the Canton | Schmid J.,Official Food Control Authority of the Canton | Marti G.,Official Food Control Authority of the Canton
European Food Research and Technology | Year: 2013

A universal cultivation media for Listeria monocytogenes, Salmonella and enteropathogenic Escherichia coli in conjunction with a highly efficient nonaplex PCR for the determination of L. monocytogenes, Campylobacter, Salmonella and enteropathogenic E. coli was developed and compared to classical microbiological assays. The achieved detection limit was at 2 colony-forming units per g for all analytes. The method allows screening of food samples within 24 h and is partly automatable. A ring trial showed that it can be easily established in other laboratories showing its robustness. The presented method yields results similar to the classical ISO methods based on cultivation. © 2013 Springer-Verlag Berlin Heidelberg.

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