Office of the State Veterinarian

Frankfort, KY, United States

Office of the State Veterinarian

Frankfort, KY, United States
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Miller M.,Palm Beach Zoo | Joubert J.,Veterinary Wildlife Services | Mathebula N.,Veterinary Wildlife Services | De Klerk-Lorist L.-M.,Office of the State Veterinarian | And 8 more authors.
Journal of Zoo and Wildlife Medicine | Year: 2012

Bovine tuberculosis (TB), caused by Mycobacterium bovis, has become established in Kruger National Park, South Africa, in the cape buffalo (Syncerus caffer) population and in other species. TB in prey species has resulted in infection and morbidity in the resident lion (Panthera leo) prides. The only validated live animal test currently available for lions is the intradermal tuberculin test. Because this test requires capture twice, 72 hr apart, of free-ranging lions to read results, it is logistically difficult to administer in a large ecosystem. Therefore, development of a rapid animal-side screening assay would be ideal in providing information for wildlife managers, veterinarians, and researchers working with free-living lion prides. This study reports preliminary descriptive results from an ongoing project evaluating two serologic tests for M. bovis (ElephantTB Stat-Pak and dual path platform VetTB). Disease status was determined by postmortem culture and presence of pathologic lesions in 14 free-ranging lions. Seropositivity was found to be associated with M. bovis infection. Extended field studies are underway to validate these rapid animal-side immunoassays for antemortem screening tests for TB in lions. © 2012 American Association of Zoo Veterinarians.


PubMed | Office of the State Veterinarian, Stellenbosch University, Ezemvelo KZN Wildlife and Veterinary Wildlife Services
Type: | Journal: Veterinary immunology and immunopathology | Year: 2016

Sporadic cases of bovine tuberculosis (bTB) have been reported in warthogs in Southern Africa and confirmed through mycobacterial culture. However, there are no validated ante-mortem tests currently available for bTB in warthogs. In this study, we evaluated the use of three serological assays for the detection of Mycobacterium bovis infection in warthogs; an indirect enzyme-linked immunosorbent assay (ELISA) using bovine purified protein derivative (PPD


Meade B.J.,University of Kentucky | Meade B.J.,Animal Plant Health Inspection Services | Timoney P.J.,University of Kentucky | Donahue J.M.,University of Kentucky | And 3 more authors.
Preventive Veterinary Medicine | Year: 2010

In 1998, a newly identified bacterium Taylorella asinigenitalis was isolated from the external genitalia and reproductive tracts of nurse mares, a stallion and donkey jacks in Kentucky. An extensive regulatory effort was implemented to contain the outbreak including the tracing and testing of 232 horses and donkeys on 58 premises. T. asinigenitalis was isolated from the reproductive tract of 10 adult equids, including two donkey jacks, one Paint Quarter-horse stallion and seven draft-type breeding mares. None of the infected horses had clinical signs of reproductive tract disease. The odds of being culture positive were 20 times greater for a mare bred to a donkey than for a mare bred to a stallion. Approximately 18% of mares bred to either a carrier stallion or donkey jack were confirmed culture positive. Seventy-one percent of infected mares required more than one course of treatment to clear the organism from their reproductive tracts and one mare harbored the organism for more than 300 days. © 2010.


Miller M.,University of Cape Town | Buss P.,Veterinary Wildlife Services | Klerk-Lorist L.-M.D.,Office of the State Veterinarian | Hofmeyr J.,Veterinary Wildlife Services | And 6 more authors.
Journal of Wildlife Diseases | Year: 2016

Warthogs (Phacochoerus africanus) have been implicated as potential maintenance hosts of Mycobacterium bovis. Our preliminary investigation of bovine tuberculosis in three warthogs describes pathologic findings and associated positive serologic results in two in- fected animals. This demonstrates the potential use of serodiagnostic tests for M. bovis infection in this species. © Wildlife Disease Association 2016.

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