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Swain S.C.,Odisha University of Agriculture and Technology | Padhi S.K.,S OA University
Pakistan Journal of Agricultural Sciences | Year: 2012

Coal ash management would remain a great concern all over the world. Several studies proposed that there is an ample scope for safe utilization of coal ash as a soil ameliorant that may improve physical, chemical and biological properties of the soil and is a source of readily available plant micro and macro nutrient. With this concept a pot culture experiment was carried out in the eastern ghat high land zone of Odisha, India under open condition in the nursery. Different levels of coal ash and soil mixture were used in different combinations to check their effect on the physio-morphological and biochemical parameters of guava. The study on the effect of varying levels of coal ash on guava revealed that the combination of 50:50 and 25:75 coal ash and soil mixture increased the seed germination, seedling characteristics, biomass, vegetative growth and chlorophyll content of the seedlings. The increase in growth traits was attributed to increase in nutrient acquisition of plants grown under above combinations. On contrary 100% coal ash in the growing medium reduced seed germination, seedling vigour, growth and biomass per plant. The leaf nutrient status of N, P, K, Ca, Mg, S and the micro nutrients Zn, Mn, B, Mo, Fe and Cu were found to be higher in the treatments having higher proportion of coal ash in the growing medium than other treatments and the lowest was recorded in control (no coal ash). The findings suggest that application of coal ash in certain proportion is beneficial in terms of growth parameters and nutrient acquisition in guava.

Misra N.,CSIR - Institute of Minerals And Materials Technology | Patra M.C.,Odisha University of Agriculture and Technology | Panda P.K.,CSIR - Institute of Minerals And Materials Technology | Sukla L.B.,CSIR - Institute of Minerals And Materials Technology | Mishra B.K.,CSIR - Institute of Minerals And Materials Technology
Journal of Biomolecular Structure and Dynamics | Year: 2013

The concept of using microalgae as an alternative renewable source of biofuel has gained much importance in recent years. However, its commercial feasibility is still an area of concern for researchers. Unraveling the fatty acid metabolic pathway and understanding structural features of various key enzymes regulating the process will provide valuable insights to target microalgae for augmented oil content. FabH (â-ketoacyl-acyl carrier protein synthase; KAS III) is a condensing enzyme catalyzing the initial elongation step of type II fatty acid biosynthetic process and acyl carrier protein (ACP) facilitates the shuttling of the fatty acyl intermediates to the active site of the respective enzymes in the pathway. In the present study, a reliable three-dimensional structure of FabH from Chlorella variabilis, an oleaginous green microalga was modeled and subsequently the key residues involved in substrate binding were determined by employing protein- protein docking and molecular dynamics (MD) simulation protocols. The FabH-ACP complex having the lowest docking energy score showed the binding of ACP to the electropositive FabH surface with strong hydrogen bond interactions. The MD simulation results indicated that the substrate-complexed FabH adopted a more stable conformation than the free enzyme. Further, the FabH structure retained its stability throughout the simulation although noticeable displacements were observed in the loop regions. Molecular simulation studies suggested the importance of crucial hydrogen bonding of the conserved Arg91 of FabH with Glu53 and Asp56 of ACP for exhibiting high affinity between the enzyme and substrate. The molecular modeling results are consistent with available experimental results on the flexibility of FabH and the present study provides first in silico insights into the structural and dynamical aspect of catalytic mechanism of FabH, which could be used for further site-specific mutagenic experiments to develop engineered high oil-yielding microalgal strains for biofuel production. Copyright © 2013 Taylor &Francis.

Mohanty M.,Sugarcane Research Station | Das P.P.,Government of Odisha | Nanda S.S.,Odisha University of Agriculture and Technology
Sugar Tech | Year: 2014

An on-farm trial in a participatory mode was conducted consecutively during 2011-2012 and 2012-2013 cropping seasons at Patuli Sahi village under Odagaon block of Nayagarh district in Odisha with a view to draw a comparative statement of the advantages of sustainable sugarcane initiative (SSI) technology of cane cultivation over the conventional three bud setts planting. Twenty-five day old seedlings were planted at 120 × 60 cm distance in SSI technology as against three bud setts planted at 75 cm row to row spacing in conventional practice. The study thus revealed that by adopting SSI technology of sugarcane cultivation, the farmers could realize a cane yield of 105 t/ha which was 18 % higher as against 89 t/ha obtained from the conventional method of cane cultivation. The cost of cultivation was Rs. 1,69,300/ha in conventional cane cultivation which came down to Rs. 1,51,950/ha when the crop was grown by SSI technology. The gross and net returns were Rs. 2,36,250 and 84,300/ha, respectively by adopting of SSI technology as compared to Rs. 2,00,250 and 30,950/ha in conventional cane farming. Sugarcane planting by SSI technology has thus proved to be more productive and economically viable since it also fetched more net returns per unit area for time invested, and can be a better option for the farmers of east coast zone of India. The SSI technology was also judged as the most sustainable by the farmers in their local agricultural production system. © 2014 Society for Sugar Research & Promotion.

Dash C.,Odisha University of Agriculture and Technology | Mohapatra S.B.,Odisha University of Agriculture and Technology
Journal of Pure and Applied Microbiology | Year: 2014

The under explored mangrove sediment of Bhitarkanika, a potent source for the isolation of new actinobacteria and were recognized as a source of novel antibiotics and anticancer agents having unusual structure and properties. In the present study twenty actinobacterial isolates were obtained and screened for L-asparaginase and L-glutaminase production. Only four isolates were found to exhibit the above activity. 16S rDNA analysis revealed one isolate i.e. DSOIIA as a new strain of Actinomycetales bacterium BkSoiiA bearing Genebank Accession number: KC573069 and positive for L-asparaginase and L-glutaminase production.

Mishra M.M.,Sambalpur University | Mohanty M.,Sambalpur University | Gulati J.M.L.,Sambalpur University | Nanda S.S.,Sambalpur University | Nanda S.S.,Odisha University of Agriculture and Technology
Indian Journal of Agricultural Sciences | Year: 2013

A field experiment was conducted consecutively for four years (2004-05 to 2007-08) at Regional Research Station, Chiplima (Sambalpur), Odisha on sandy loam soil with pH 6.0 and organic carbon content of 0.56%. The available N, P and K were 212, 15.7 and 89 kg/ha respectively. The experiment was laid out in randomized block design involving 10 rice based crop sequences with three replications. Among various cropping sequences evaluated, rice (Oryza sativa L.)- frenchbean (Phaseolus vulgaris)-greengram (Vigna radiata L.) produced the highest rice equivalent yield (17.31 tonnes/ ha/year), net return (ruppes 39 899/ha), benefit: cost ratio (2.25) and monetary advantage (140.48 ruppes/ha/day). This system was the most sustainable with sustainable yield index of 0.40 and sustainable value index of 0.84 with higher energy productivity (0.854 kg/MJ) and energy intensiveness (0.078 MJ/Re). However, the rice- groundnut-sesame system had highest land use efficiency of 87.14% whereas rice-radish-greengram registered the highest production efficiency (61.54 kg/ha/day). Rice- groundnut - greengram sequence was found to be the most efficient user of N (138.8 kg yield/ha N applied) whereas rice - groundnut - fallow used P (388.3 kg yield/ha N applied) and K (202.6 kg yield/ha N applied) more efficiently than that of other cropping sequences. The study further revealed that rice productivity could be enhanced by 19.1 and 17.7% due to inclusion of oilseeds and pulses, respectively than that of its monocropping..

Mohanty M.,Odisha University of Agriculture and Technology | Nanda S.S.,Odisha University of Agriculture and Technology | Barik A.K.,Odisha University of Agriculture and Technology
Indian Journal of Agricultural Sciences | Year: 2013

The experiment was conducted during kharif seasons of 2007-08 and 2008-09 at Central Research Station of the Odisha University of Agriculture and Technology, Bhubaneshwar on sandy loam, well drained upland lateritic soil. The experimental soil was low in organic carbon (0.43%), N (228 kg/ha) and K (122 kg/ha) and medium in available P (18 kg/ha). The experiment was laid out in randomized block design with twelve treatments each replicated thrice. The test variety of rice, RGL 2538 (Vasundhara), was raised following the recommended package of practices. Application of 1/3rd recommended dose (RD) of N each through chemical fertilizer; FYM and Azolla registered the highest plant height and leaf area index in rice (Oryza sativa L.) as compared to other treatment combinations. Higher yield components (viz. number of panicles/m 2, number of filled grains/panicle) and grain and straw yield of rice were also achieved from the same treatment as compared to 100% recommended dose of fertilizer and control. This was at par with the application of 50% RDN as chemical fertilizer + 50% RDN either as dhaincha or Azolla. N and P uptake by rice was highest with the use of 1/3rd N each as chemical fertilizer, FYM and Azolla, but higher K uptake was reported with application of 50% N as chemical fertilizer and 50% N as dhaincha. The highest gross return, net return and return per rupee investment were achieved from rice supplied with 1/3 rd N each as chemical fertilizer, FYM and Azolla. Gross return and net return were significantly superior to that of 100 % chemical fertilizer alone. However, return per rupee investment was at par with that of sole use of chemical fertilizers due to lower cost of cultivation incurred in chemical farming practices.

Pradhan A.K.,Siksha ‘O’ Anusandhan University | Kar S.K.,Siksha ‘O’ Anusandhan University | Mohanty M.K.,Odisha University of Agriculture and Technology
International Conference on Electrical, Electronics, Signals, Communication and Optimization, EESCO 2015 | Year: 2015

The stand-alone hybrid renewable power generation systems usually have lower costs with higher reliability than solar photovoltaic (PV) or wind energy system only. The most usual hybrid systems are PV/Wind/Battery and PV/Diesel/Battery. The excess energy is stored usually in lead-acid type of batteries. The optimization design is carried out by minimizing the unit cost of energy (UCOE) and GHG (Green House Gas) emissions (CO2, NOx and particles) for the various cases, comparing the results obtained by means of HOMER (Hybrid Optimization Model of Electric Renewable) software. In this paper, the optimal cost analysis of hybrid energy system is based on the load profile, solar irradiance, wind speed and price of fuel, which is collected from an un-electrified village near Bhubaneswar, Odisha in India. Moreover, the optimization of the system is obtained by varying the sensitivity variables like solar radiation and wind speed data. The cash flow summary of the hybrid system is obtained which will be useful for the optimal cost allocation of each individual component utilized in the system. © 2015 IEEE.

Singh S.N.,Indian Institute of Sugarcane Research | Chauhan R.S.,Indian Institute of Sugarcane Research | Kumar R.,U P Council Of Agricultural Research | Patnaik J.R.,Odisha University of Agriculture and Technology
Sugar Tech | Year: 2016

In India, the ratoon cane productivity at national level stands at 58 t/ha against 85 t/ha for plant cane. Ratooning is a practice of growing full crop of sugarcane from sprouts of underground stubble buds left in the field after harvest of the plant (main) crop. In sugarcane farming, ratooning saves the cost of seedbed preparation, seed material and planting operations. Ratoons help in extending the crushing period of sugar mills as they mature earlier than the plant crop. However, most often, ratoon crop yields are lower than the plant crop. The major cause of low ratoon cane productivity in north-west Indian subtropics is the prevalence of severe winter conditions coinciding with harvesting of early-maturing high sugar varieties which inhibit the sprouting of subterranean stubble buds. Unsprouted stubble buds cause gaps in subsequent sugarcane (Saccharum sp Hybrid) ratoon crop resulting in lower initial plant stand and poor crop yield. Several agro-techniques viz., trash mulching, polyethylene mulching and intercropping have been applied to enhance the bud sprouting in winter-initiated ratoon, but all the efforts could not produce the desired results. To address these constraints, a field experiment was conducted during 2009–2010 and 2010–2011 for three consecutive seasons at the Indian Institute of Sugarcane Research, Lucknow, India, to assess the effect of cultural package practices and organic and/or inorganic sources of additions on yield and quality of winter-initiated ratoon in Indian subtropics. Results thus showed that application of sulphitation press mud cake (SPMC) at 20 t/ha or SPMC at 10 t/ha + 25 kg zinc sulphate improved the bud sprouting and significantly produced maximum number of shoots. Significant increase in the number of millable canes was noticed with the application of SPMC at 20 t/ha (103.1 thousand/ha) and SPMC 10 t/ha + 25 kg ZnSO4 (102.1 thousand/ha). Maximum cane yield (71 t/ha) was also recorded with the application of SPMC at 20 t/ha followed by SPMC at 10 t/ha + 25 kg ZnSO4 (69.3 t/ha). Thus, it clearly suggests that productivity of winter-initiated ratoon can be enhanced through the application of SPMC at 20 t/ha or SPMC 10 t/ha + 25 kg ZnSO4/ha since these applications at the time of ratoon initiation enhanced the ratoon cane yield by 16.34 and 14.29 %, respectively, than that of conventional package of practices for ratoon initiation in subtropical climatic conditions of India. Juice quality of ratoon cane was remained unchanged. © 2016 Society for Sugar Research & Promotion

PubMed | National Dairy Research Institute, SASTRA University, Institute of Life science, University of Nebraska Medical Center and 2 more.
Type: Journal Article | Journal: Oncotarget | Year: 2016

Desmoplasia in human pancreatic cancer (PC) promotes cancer progression and hinders effective drug delivery. The objectives of this study were to characterize a homologous orthotopic model of PC in Syrian golden hamster and investigate the effect of anti-fibrotic (pirfenidone), antioxidant (N-acetyl cysteine, NAC) and anti-addiction (disulfiram, DSF) drugs on desmoplasia and tumor growth in this model. The HapT1 PC cells when implanted orthotopically into hamsters formed tumors with morphological, cellular and molecular similarities to human PC. Protein profiling of activated hamster pancreatic stellate cells (ha-PSCs) revealed expression of proteins involved in fibrosis, cancer cells growth and metastasis. Pirfenidone, suppressed growth of HapT1 cells and the desmoplastic response in vivo; these effects were enhanced by co-administration of NAC. Disulfiram alone or in combination with copper (Cu) was toxic to HapT1 cells and PSCs in vitro; but co-administration of DSF and Cu accelerated growth of HapT1 cells in vivo. Moreover, DSF had no effect on tumor-associated desmoplasia. Overall, this study identifies HapT1-derived orthotopic tumors as a useful model to study desmoplasia and tumor-directed therapeutics in PC. Pirfenidone in combination with NAC could be a novel combination therapy for PC and warrants investigation in human subjects.

Mohanty M.,Odisha University of Agriculture and Technology | Nanda S.S.,Odisha University of Agriculture and Technology | Barik A.K.,Odisha University of Agriculture and Technology | Barik A.K.,Visva Bharati University
Indian Journal of Agricultural Sciences | Year: 2014

A field experiment was conducted consecutively for two cropping seasons of 2007-08 and 2008-09 to study the effect of organic (FYM: farmyard manure, vermicompost and neem cake) and inorganic (chemical fertilizers) sources of plant nutrients at different combinations on the growth and yield of potato (Solanum tuberosum L.) tubers and economics besides assessing its impact on soil health in terms of available OC, N,P and K status after its harvest during both the seasons. The experimental soil was slightly acidic (pH 6.4) in nature, low in organic carbon (0.481%), available N (228 kg/ha) and medium in available K (122 kg/ha) and P (18 kg/ha). Results indicated that the application of 50% recommended dose of fertilizers (RDF) as inorganic sources in conjunction with rest 50% RDF as organic sources as FYM or vermicompost or neem cake (T6, T7 and T8) 15 days before final land preparation, registered significantly higher tuber growth, tuber bulking rate, weight of tubers/plant and yield of potato tubers as compared to rest of the treatments including 100% RDF as inorganic sources alone. Accordingly, the above treatments, on an average, recorded higher tubers yield (19.65 t/ha) to the tune of 6.72, 11.70 and 57.71% than that of 75% RDF as inorganic fertilizers + 25% as organic manures (FYM or vermicompost or neem cake), 100% RDF as chemical fertilizers and control (no manure or chemical fertilizer), respectively. Moreover, T6, T7 and T8 treatments also exhibited higher uptake of N, P and K from soils in addition to more net returns (Rs. 56993/ha) and benefit: cost ratio (1.94) as compared to rest of the treatments. Integrated nutrient management approach not only proved beneficial in enhancing the yield of potato, but also improved the contents of available organic carbon, N, P and K in treatments where 50% recommended dose of NPK was applied through inorganic and remaining 50% RDF through organic sources.

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