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Nicholas S.A.,University of Kent | Bubnov V.V.,Odessa State Medical University | Yasinska I.M.,University of Kent | Sumbayev V.V.,University of Kent
Cellular and Molecular Life Sciences | Year: 2011

Inflammatory reactions to ssRNA viruses are induced by the endosomal Toll-like receptors (TLRs) 7 and 8. TLR7/8-mediated inflammatory reaction results in activation of the Nalp3 inflammasome via an unknown mechanism. Here we report for the first time that TLR7/8 mediate activation of xanthine oxidase (XOD) in an HIF-1α-dependent manner. XOD produces uric acid and reactive oxygen species, which could activate Nalp3 and therefore induce activation of caspase 1, known to convert inactive pro-IL-1β into active IL-1β. Specific inhibition of the XOD activity attenuates TLR7/8-mediated activation of caspase 1 and IL-1β release. These results were obtained using human THP-1 myeloid macrophages. The findings were verified by conducting in vivo experiments on mice. © 2010 Springer Basel AG. Source

Botla S.K.,German Cancer Research Center | Gholami A.M.,TU Munich | Malekpour M.,Tehran University of Medical Sciences | Malekpour M.,Vanderbilt University | And 15 more authors.
Breast Cancer Research and Treatment | Year: 2012

DNA methylation patterns have been recognised as cancer-specific markers with high potential for clinical applications. We aimed at identifying methylation variations that differentiate between breast cancers and other breast tissue entities to establish a signature for diagnosis. Candidate genomic loci were analysed in 117 fresh-frozen breast specimens, which included cancer, benign and normal breast tissues from patients as well as material from healthy individuals. A cancer-specific DNA methylation signature was identified by microarray analysis in a test set of samples (n = 52, p<2.1 × 10 -4) and its performance was assessed through bisulphite pyrosequencing in an independent validation set (n = 65, p<1.9 × 10-7). The signature is associated with SFRP2 and GHSR genes, and exhibited significant hypermethylation in cancers. Normal-appearing breast tissues from cancer patients were also methylated at these loci but to a markedly lower extent. This occurrence of methylated DNA in normal breast tissue of cancer patients is indicative of an epigenetic field defect. Concerning diagnosis, receiver operating characteristic curves and the corresponding area under the curve (AUC) analysis demonstrated a very high sensitivity and specificity of 89.3 and 100 %, respectively, for the GHSR methylation pattern (AUC >0.99). To date, this represents the DNA methylation marker of the highest sensitivity and specificity for breast cancer diagnosis. Functionally, ectopic expression of GHSR in a cell line model reduced breast cancer cell invasion without affecting cell viability upon stimulation of cells with ghrelin. Our data suggest a link between epigenetic down-regulation of GHSR and breast cancer cell invasion. © Springer Science+Business Media, LLC. 2012. Source

Goreshnik E.A.,Jozef Stefan Institute | Gelmboldt V.O.,Odessa State Medical University | Koroeva L.V.,Physico Chemical Institute of Environment and Human Protection | Ganin E.V.,Odessa State Environmental University
Journal of Fluorine Chemistry | Year: 2011

3,5-Diamino-1,2,4-triazolium tetrafluoroborate (LH)BF4 and hexafluorosilicate (LH)2SiF6 have been isolated and characterized by single-crystal X-ray structure determination, IR spectroscopy, mass spectrometry, solubility data, potentiometry. The N-H⋯F and N-H⋯N hydrogen bonds play an important role in a formation of 3-D structures of two compounds. The relationship between the salts structure and some properties is discussed. © 2010 Elsevier B.V. All rights reserved. Source

Gelmboldt V.O.,Odessa State Medical University | Ganin E.V.,Odessa State Environmental University | Koroeva L.V.,Ukrainian Academy of Sciences | Botoshansky M.M.,Technion - Israel Institute of Technology | Fonari M.S.,Moldova Academy of Sciences
Journal of Fluorine Chemistry | Year: 2010

Interaction of hexafluorosilicic acid with sulfa drugs sulfathiazole (stz) and sulfalen (2-sulfanilamido-3-methoxypyrazine, sl) results in the crystalline salts of the compositions [stzH]2[SiF6] (I) and [4-H 2NO2SPhNH3]2[SiF6] (II). Complex I is characterized by IR, mass spectrometry data and single crystal X-ray diffraction. The crystal structure of I is stabilized by a network of charge-assisted hydrogen bonding. The relationship between the solubility and H-bonding system in I, II and related "onium" hexafluorosilicates is discussed. The formation of complex II, previously reported as an interaction product of hexafluorosilicic acid with 4-aminobenzenesulfonamide (sulfanilamide), is the result of cleavage of the C-N bond in sulfalen in acidic medium.© 2010 Elsevier B.V. All rights reserved. Source

Orlovskaya S.G.,Odessa I I Mechnikov National University | Kalinchak V.V.,Odessa I I Mechnikov National University | Zuy O.N.,Odessa I I Mechnikov National University | Mandel O.V.,Odessa State Medical University | Kachan S.V.,The National Pedagogical University
Ukrainian Journal of Physics | Year: 2011

The influence of internal reactions on the characteristics of hightemperature heat and mass transfer and the critical conditions for the ignition and the extinction in gas suspensions of carbon particles are researched. It is found that the internal reactions lead to a significant decrease in the ignition delay and critical diameters, which determines the ignition and the extinction of particles of a gas suspension. It is proven that the porous particle extinguishes when its mass achieves some critical value. The intervals of initial diameters and mass concentrations of particles are determined corresponding to the most complete burnout of carbon fuel. © 2011. Source

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