Odessa National Medical University

Nova Odesa, Ukraine
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Objective: The study of the assimilation of glucose and maltose in the small intestine of two generations of posterity of irradiated male rats received with a food the milled milk thistle fruits after irradiation. Methods: Exposition of male rats performed once by the help of telegamma-setting "AGAT-R1", dose was 120 rad/min, field 20×20, distance from the source of irradiation to the field=75 cm, dose-0.5 Gy, exposure - 32 sec. Accumulating mucosa preparations (APS) produced by the method of Ugolev et al. APS incubated for 1 hour at t=37°C in the oxygenated medium. As the incubation medium were used solutions 10 mmol/l of glucose and 5 mmol/l of maltose that were made on the Ringer solution pH 7.4. In all incubation medium the bile was added. The concentration of free glucose and M-glucose formed due to the hydrolysis of maltose was determined using method described in ref. Colorimetrically on photoelectrocolorimeter-CFC- 2MP, λ=625 nm. The statistical processing of the obtained data was conducted using "Primer Biostatistics" software. Results: The 2nd generation of the offspring from the males, which received milk thistle in the food immediately after irradiation, showed the high activity of the transport system for the free glucose and system providing transport of M-glucose produced by the hydrolysis of maltose both. This activity is higher than in the corresponding groups of the 1st generation (p<0,0001) and higher than corresponding data in all experimental groups of 2nd generation (except only group of the posterity of the male rats got milk thistle fruits before irradiation). Conclusion: The use by predecessors of milk thistle after irradiation leads to the radio corrective effect on the systems of transport of glucose and maltose both exactly in second generation of their posterity.

Objective - to develop the form of the drug reservoir to hold the vegetable oils under the basis of the prosthesis. The research was conducted on the Bench PMMA samples. The comparison group consisted of smooth plate PMMA. Two study groups were PMMA plates with grooves on one side of a square with sides of 2 mm and depth of channel - 1 mm. The third group consisted of the experimental plate with diamond-shaped notches with the same parameters. The lowest value of the stress in the plate having a rhombic grid, they are 54% less than in the smooth plate and 37% less than in the plate with a square lattice (the best strength characteristics in a rhombic plate with grille). Equivalent move from the plate with a rhombic lattice is less than an order of magnitude than that of a smooth plate and by 5.8% more than at the plate with a square lattice, which indicates good performance design plasticity with rhombic grid. Basis with the rhombic lattice on the surface has the best record on the stress-strain state in comparison with other models considered (smooth plate with a square lattice plate).

Fainerman V.B.,SINTERFACE Technologies | Trukhin D.V.,Odessa National Medical University | Zinkovych I.I.,Donetsk National Medical University | Miller R.,Max Planck Institute of Colloids and Interfaces
Advances in Colloid and Interface Science | Year: 2017

Dynamic surface tensions and dilational visco-elasticity are easy accessible parameters of liquids. For human body liquids, such as urine, blood serum, amniotic fluid, gastric juice, saliva and others, these parameters are very characteristic for the health status of people. In case of a disease the composition of certain liquids specifically changes and the measured characteristics of dynamic surface tension of the dilational surface elasticity and viscosity reflect these changes in a clear way. Thus, this kind of physico-chemical measurements represent sensitive tools for evaluating the severity of a disease and can serve as control tool for the efficiency of applied therapies. The overview summarises the results of a successful work over about 25. years on this subject and gives specific insight into a number of diseases for which the diagnostics as well as the therapy control have been significantly improved by the application of physico-chemical experimental techniques. © 2017 Elsevier B.V.

Grubnik V.V.,Odessa National Medical University | Grubnik A.V.,Odessa National Medical University | Vorotyntseva K.O.,Odessa National Medical University
Wideochirurgia I Inne Techniki Maloinwazyjne | Year: 2014

Introduction: Laparoscopic incisional and ventral hernia repair (LIVHR) was first reported by Le Blanc and Booth in 1993. Many studies are available in the literature that have shown that laparoscopic repair of incisional and ventral hernia is preferred over open repair because of lower recurrence rates (less than 10%), less wound morbidity, less pain, and early return to work. Aim: To identify the long-term outcomes between the different types of meshes and two techniques of mesh fixation, i.e., tacks (method Double crown) and transfascial polypropylene sutures. Material and methods: A total of 92 patients underwent LIVHR at our department between January 2009 and August 2012. The hernias were umbilical in 26 patients, paraumbilical in 15 patients and incisional in 51 patients. All patients admitted for LIVHR were randomized to either group I (tacker fixation of ePTFE meshes) or group II (suture fixation of meshes with nitinol frame) using computer-generated random numbers with block randomization and sealed envelopes for concealed allocation. Results: The mean mesh fixation time was significantly higher in the tacker fixation group (117 ± 15 min vs. 72 ± 6 min, p < 0.01). There were no conversions in either group. The median postoperative hospital stay was 3.5 ± 1.5 days. All patients were followed up at 1, 3, 6, 12 and every 6 months thereafter postoperatively. There were 5 recurrences in the study population. In group I there were 4 patients with recurrence, and only 1 patient in the group with meshes with a nitinol frame. Conclusions: Meshes of the new generation with a nitinol framework can significantly improve laparoscopic ventral hernia repair. The fixation of these meshes is very simple using 3-4 transfascial sutures. The absence of shrinkage of these meshes makes the probability of recurrence minimal. Absence of tackers allows postoperative pain to be minimized. We consider that these new meshes can significantly improve laparoscopic ventral hernia repair.

We studied the pathogenetic role of central structures belonging to epileptogenic and antiepileptogenic systems, namely the ventral hippocampus (VH), superior colliculus (SC), and frontal cortex, in the pathogenesis of pilocarpine-induced status epilepticus (with the presence of spontaneous seizures, SSs). Using a stereotaxic technique, the above-mentioned structures were subjected to preliminary local destruction (by microinjections of ibotenic acid) or electrostimulation in the course of the experiment. Pilocarpine-induced SSs were readily recorded in rats subjected to stimulation of the VH and frontal cortex, as well as to destruction of the SC. At the same time, in rats with destruction of the VH and frontal cortex but with activation of the SC, we observed no SS episodes. Therefore, the above-mentioned central structures play significant pathogenetic roles in the mechanisms underlying the development of the pilocarpine-induced epileptic state; the VH and frontal cortex are in reciprocal relations with the SC. © 2014 Springer Science+Business Media New York.

Gelmboldt V.O.,Odessa National Medical University | Gelmboldt V.O.,Ukrainian Academy of Sciences
Russian Journal of Inorganic Chemistry | Year: 2012

The relationship between the water solubility of hexafluorosilicates with different types of onium cations and the characteristics of interionic H-bonds in their structures was analyzed. The antibate correlation between the solubility and the number of short interionic H-bonds was revealed for salts with aromatic heterocyclic and arylammonium cations. © Pleiades Publishing, Ltd., 2012.

Gel'Mbol'Dt V.O.,Odessa National Medical University
Russian Journal of Inorganic Chemistry | Year: 2014

The known data regarding the effects of interionic hydrogen bonding on properties of onium hexafluorosilicates with heterocyclic cations are summarized. Thermal stability parameters and water solubilities for this type of salts are shown to correlate with the number of strong and medium-strength hydrogen bonds or the number of hydrogen donors in the salt structure. © 2014 Pleiades Publishing, Ltd.

Kresyun N.V.,Odessa National Medical University
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i | Year: 2014

AIM: The characteristics of visual evoked potentials (VEP) have been studied in diabetic patients with and without diabetic retinopathy.MATERIAL AND METHODS: Magnetic impulses (2.0 T1 at the height of impulse) have been delivered to the cerebellar surface transcranially using the "Neuro-MS/D", (Russia Federation). Delta- sleep inducing peptide ("Deltalycyn", "Biopharma", Russia Federation) was intranasally delivered in 30 min before photostress. Afterwards VEP have been registered every 20 s from the moment of photo stress during one minute.RESULTS: An increase of the latency period and a reduction of the VEP amplitude have been recorded in the period following photo stress exposure of the macular part of the retina. The VEP characteristics restored to the initial level in 73.5 +/- 3.3 s from the photo stress moment in the control group; while in diabetic patients with retinopathy this index was 137.2 +/- 11.3 s. In the. presence of cerebellar transcranial magnetic stimulations (2.0 T1, 20 impulses) the VEP amplitude depression was less pro- nounced, and the restoration period of the VEP characteristics shortened to 110.3+ 12.7 s, while in deltalycyn treated patients restoration was observed in 95.1+ 6.8 s. Under condition of combined usage of deltalycyn and TMS period of restoration of VEP was shortened up to 82.5 +/- 6.5 s.CONCLUSIONS: Retinopathy development is linked to prolonged VEP latency period (P100), lowering of the N75-P100 amplitude, as well as to enlargement of the recovery period of the retina's functional capacity in patients suffering from the diabetes mellitus in the presence of photo stress. Superlatively administered deltalycyn and cerebellar transcranial magnetic stimulation facilitates a faster recovery of the retina's functional capacity in response to photo stress in diabetic patients with retinopathy. 3. Combined usage of deltalycyn and cerebellar transcranial stimulation caused the potentiated shortening of post-photo stress recovering of VEP in patients with diabetic retinopathy.

Matyushkina M.V.,Odessa National Medical University
Neurophysiology | Year: 2014

Neurotropic effects of new coordination compounds, germacit and stanmacit, were tested on models of acute generalized convulsive activity induced in rats by injections of pentylenetetrazol (PTZ, 40 mg/kg), picrotoxin (PT, 2.0 mg/kg), or kainic acid (KA, 15 mg/kg). The tested biologically active substances were injected in doses corresponding to 1/10, 1/20, 1/40, 1/80, or 1/135 of the calculated LD50. Germacit provided obvious anticonvulsive effects in the case of seizure activity induced by PTZ (in a dose of 1/80 LD50), PT, and KA (in doses of 1/40 LD50); the intensity of convulsive manifestations decreased significantly compared with analogous indices in control groups (P < 0.05). Stanmacit did not influence the expression of acute PT-induced convulsions (P > 0.05) but intensified PTZ- and KA-induced seizures (at 1/20 and 1/40 LD50, respectively; P < 0.05). The obtained data are indicative of the ability of new complex coordination compounds of germanium and tin with magnesium and citric acid to significantly control the excitability of cerebral structures involved in the generation of epileptiform activity. © 2014, Springer Science+Business Media New York.

Grubnik V.V.,Odessa National Medical University | Malynovskyy A.V.,Odessa National Medical University
Surgical Endoscopy and Other Interventional Techniques | Year: 2013

Background Mesh repair may decrease the recurrence rate but bears risk of esophageal complications. This study aimed to analyze the long-term results of laparoscopic hiatal repair depending on hiatal surface area (HSA). Methods The results from 658 procedures were analyzed. Group 1 had 343 patients with HSA smaller than 10 cm2 (small hernias), for whom primary crural repair was performed. Group 2 had 261 patients with HSA size 10-20 cm2 (large hernias), for whom primary crural repair (subgroup A) or mesh repair (subgroup B) was performed. Group 3 had 54 patients with HSA larger than 20 cm2 (giant hernias), for whom only mesh repair was performed. Results The mean follow-up period was 28.6 months (range, 10-48 months). Primary repair results in a higher recurrence rate for large hernias (11.9 %) than for small hernias (3.5 %) (p = 0.0016). For large hernias, the original method of sub-lay lightweight partially absorbable mesh repair provides a lower recurrence rate than primary repair (4.9 % vs 11.9 %; p = 0.0488) and a comparable dysphagia rate (2.1 % vs 2.2 %; p = 0.6533). For giant hernias, mesh repair results in a higher recurrence rate than for large hernias (20 % vs 4.9 %; p = 0.0028). The analysis of variance (ANOVA) HSA recurrence ratio confirmed the correctness of the chosen threshold levels (10 and 20 cm2) for subdividing hernias into three classes according to the new classification. Conclusions The authors advise routine measurement of HSA and use of relative classification, primary suturing as the optimal repair for small hernias, the original technique of sub-lay lightweight partially absorbable mesh repair as the apparent best treatment for large hernias, and the original technique for giant hernias, which provides results corresponding to those reported in the literature, although these results require improvement. © Springer Science+Business Media New York 2013.

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