Occupational Health Institute

Clermont-Ferrand, France

Occupational Health Institute

Clermont-Ferrand, France

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Rabiau N.,Jean Perrin Center | Rabiau N.,University of Auvergne | Rabiau N.,Soluscience S.A. | Trraf H.-K.,Jean Perrin Center | And 16 more authors.
In Vivo | Year: 2011

Background: MicroRNAs (miRNAs) are small non-coding RNAs that have aberrant expression in prostate cancer tissues. miRNAs are involved in the initiation and progression of cancer, and several miRNAs have been characterized as tumor suppressors or oncogenes. It has been shown that some miRNAs can be directly regulated from their own promoters by epigenetic alterations in cancer cells. Moreover, phytoestrogens are known to have epigenetic action on gene transcription. Hence, we conducted here an examination of the miRNA expression profile in human prostate cancer cell lines after soy phytoestrogen treatment. Materials and Methods: The comparative miRNA expression profiles of prostate cell lines (PC-3, DU145, LNCaP) after a 48-h treatment of 40 μM genist ein, 110 μM daidzein, or 2 μM 5-azacytidine (5-AZA, a demethylating agent) were conducted with a Taqman low-density array. Results: We found that out of 377 miRNAs tested, 180, 170 and 150 miRNAs were amplified with 2% of variation in the triplicate in PC-3, DU145 and LNCap cells, respectively, and only 5 miRNAs for PC-3 and DU145 cells and 4 miRNAs for LNCap exhibited a significant change in their expression. Treatment with genistein or daidzein had similar effects on miRNA regulation to those of 5-AZA treatment. Conclusion: This work demonstrated a new role of isoflavones on the regulation of miRNAs in prostate cancer.


Vardi A.,Center Jean Perrin | Bosviel R.,Center Jean Perrin | Rabiau N.,Center Jean Perrin | Rabiau N.,Soluscience S.A. | And 13 more authors.
In Vivo | Year: 2010

Background: The aim of this study was to determine the effects of soy phytoestrogens on the methylation of promoter genes in prostate tumors. The incidence of prostate cancer in Asia is thirty percent lower than in Western countries. Since soy phytoestrogens represent a large portion of the Asian diet, evidence suggests their protective effect against prostate cancer. Materials and Methods: In three human prostate cancer cell lines, methylation-specific-PCR was used to determine the effect of soy isoflavones (genistein and daidzein), compared to known demethylating agent 5-azacytidine as control in the promoter regions of glutathione S-transferase P1 (GSTP1), Ras association domain family 1 (RASSF1A), ephrin B2 (EPHB2) and breast cancer 1 (BRCA1) genes. In parallel, immunohistochemistry was used to assess the effects of genistein, daidzein and 5-azacytidine treatment on the corresponding protein expression. Results: All studied promoters, with the exception of that for BRCA1, were strongly methylated without treatment. After treatment by phytoestrogens, demethylation of GSTP1 and EPHB2 promoter regions was observed and an increase in their protein expression was demonstrated by immunohistochemistry. Conclusion: Epigenetic modifications of DNA, such as the promoter CpG island demethylation of tumor suppressor genes, might be related to the protective effect of soy on prostate cancer.


Kokkonen A.,University of Eastern Finland | Hyttinen M.,University of Eastern Finland | Holopainen R.,Occupational Health Institute | Salmi K.,Occupational Health Institute | Pasanen P.,University of Eastern Finland
Indoor and Built Environment | Year: 2014

The ventilation performance of airborne infection isolation rooms (AIIRs) was assessed in three Finnish hospitals by examining the air change rate, contaminant removal efficiency and leakage of contaminants outside the isolation room by using tracer gas techniques. Results showed that infectious agents can escape from the AIIR during egress despite high ventilation rates in the AIIR and anteroom (air change rate, 4-24 h-1) and the pressure difference between the AIIR and corridor was 0.2 to 29 Pa. The control of impurities was often ineffective due to inappropriate direction of air flows and air moving from the patient towards the health care worker and anteroom. Although an anteroom reduces leakage of infectious agents to the corridor significantly, it does not prevent this completely when healthcare workers move between the AIIR and corridor. To enhance the protection of AIIRs, it is especially important to pay attention to the air distribution and removal efficiency of impurities in AIIRs and anterooms. Performance of AIIRs should be tested regularly, especially among older AIIRs. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

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