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İskenderun, Turkey

Turgut O.,Gynecology and Obstetrics Clinic | Ay A.A.,Kirikkale University | Turgut H.,Cardiology Clinic | Ay A.,General Surgery Clinic | And 2 more authors.
Age | Year: 2013

The purpose of the study was to assess whether it is possible to reduce the oxidative damage using antioxidant agents combined with hormone replacement therapy after menopause. In this prospective experimental study, 50 mature femaleWistar albino rats weighing 270-310 g were used. Rats were divided into the following six groups: (1) Ovx group (n=7): The animals underwent bilateral ovariectomy. No drug was administered following bilateral ovariectomy. (2) Ovx+ E2 group (n=7): bilateral ovariectomy+17ß-estradiol (100 μg/kg/day); (3) Ovx+E2+MT5 group (n=7): bilateral ovariectomy+17ß-estradiol (100 μg/kg/day)+melatonin (5 mg/kg/day); (4) Ovx+E2+MT20 group (n=7): bilateral ovariectomy+17ß-estradiol (100 μg/kg/day)+ melatonin (20 mg/kg/day); (5) Ovx+E2+Dxp250 group (n = 7): bilateral ovariectomy + 17ß-estradiol (100 μg/kg/day)+dexpanthenol (250 mg/kg/day); (6) Ovx+E2+Dxp500 group (n=7): bilateral ovariectomy+ 17ß-estradiol (100 μg/kg/day)+dexpanthenol (500 mg/kg/day), and the activity of these antioxidative enzymes and oxidative stress products were measured. Enzymatic activity levels of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase(GSH-Px), and glutathione reductase and levels of free radicals (malondialdehyde (MDA) and nitric oxide) were both analyzed. We observed an increase in the level of GSH activity, but no significant differences in levels of CAT, SOD, and GSH-Px enzymatic activity and in levels of free radical MDA following 17ß-estradiol or additional antioxidant treatment (melatonin or dexpanthenol). Despite the present study indicating that the addition of melatonin and dexpanthenol into the hormone replacement therapy regimen may contribute to the antioxidant effect of estrogen, the existence of limited data in this field indicates that further studies are warranted. © American Aging Association 2013. Source

Borsa B.A.,Istanbul Kemerburgaz University | Ozcun G.,Baskent University | Houbraken J.,Fungal Biodiversity Center | Okmen F.,Gynecology and Obstetrics Clinic
Mikrobiyoloji Bulteni | Year: 2015

The vast majority of vaginal fungal infections are caused by Candida species. However, vaginitis cases caused by molds are extremely rare. Aspergillus protuberus is previously known as a member of Aspergillus section Versicolores which can cause opportunistic infections in immunocompromised patients, however it has recently been described as a seperate species. Although the members of Aspergillus section Versicolores have been isolated rarely in cases of pulmonary infections, eye infections, otomycosis, osteomyelitis and onycomycoses, to the best of our knowledge, there is no published case of human infection caused by A.protuberus. In this report, the first case of persistent vaginitis due to A.protuberus in an immunocompetent patient was presented. A 42-year-old female patient was admitted to our hospital with the complaints of pelvic pain, vaginal itching and discharge during one month. Her symptoms had been persistant despite of the miconazole nitrate and clotrimazole therapies for probable candidal vaginitis. Fungal structures such as branched, septate hyphae together with the conidial forms were seen in microscopic examination as in the cervical smear. Thereafter, a vaginal discharge sample was taken for microbiological evaluation and similar characteristics of fungal structures were observed in the microscopic examination as of cervical smear. Then, preliminary result was reported as Aspergillus spp. At the same time, the sample was plated on Sabouraud dextrose agar (SDA) in duplicate and incubated at room temperature and at 37oC. After 5 days, white, powdery and pure-looking fungal colonies were observed in SDA which was incubated at room temperature, while the other medium remained sterile. The culture was submitted to the CBS-KNAW Fungal Biodiversity Center for further characterization. Phenotypic identification showed that the isolated strain belonged to the Aspergillus section Versicolores. The strain was grown for 7 days on malt extract agar and then ITS regions were amplified and sequenced from isolated DNA for genomic characterization. The obtained sequences were compared with the NCBI database and internal databases of the CBS-KNAW Fungal Biodiversity Centre and confirmed as Aspergillus section Versicolores. As a result of recent changes in classification of fungi, analysis of partial fS-tubulin and calmodulin sequences have also been used to obtain a detailed and precise characterization. Eventually, the strain has been identified as A.protuberus which is a recently accepted species distinct from Aspergillus section Versicolores. As the patient could not be contacted after the preliminary report, detailed demographical information, probable origin and route of transmission of the agent and prognosis of infection remained obscure. In conclusion, the first case of vaginitis caused by A.protuberus was described in this report with the support of clinical, pathological, microbiological and molecular data. Source

Arteni A.,Gynecology and Obstetrics Clinic | Fabiani G.,Gynecology and Obstetrics Clinic | Marchesoni D.,Gynecology and Obstetrics Clinic
Giornale Italiano di Ostetricia e Ginecologia | Year: 2014

Autoimmune diseases affect more frequently women. Hormonal variations in menopause modify the incidence and trend of autoimmune diseases. The dramatic decrease in estrogen levels due to ovarian exhaustion and the constant DHEA production are regarded as protective hormonal factors even though menopause has multifactorial traits: age, age at menopause onset, duration and gravity of the disease, estrogen receptor alteration, genetic alteration, interaction between estrogen and androgen receptors, progesterone and prolactin. Hormone replacement therapy (HRT) does not seem to be linked to relapses nor to a new disease in patients with rheumatoid arthritis. It is quite controversial though the use of estrogens in the presence of systemic lupus erythematosus. In this case, the risk of low to medium disease relapse in women undergoing HRT is quite significant. The use of NOR progesterone derivates and of transdermal estrogen can be more effective than E.E.C.-M.A.P system. SERMs have proven to be an effective option for the treatment of osteoporosis which is often linked to autoimmune diseases. Tibolone appears to show tissue specific androgen activity with no added mammary risk. HRT needs to be administered in low dosage to patients with autoimmune diseases and for no longer than 2 years (WHI). An alternative to HRT are: microdose TTS estrogen therapy for osteoprotection, topical estrogen for genital dystrophy, progestins for vasomotor syndrome. © Copyright 2014, CIC Edizioni Internazionali. Source

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