Observatory of Herpetology

Donostia / San Sebastián, Spain

Observatory of Herpetology

Donostia / San Sebastián, Spain
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Vences M.,TU Braunschweig | Sarasola-Puente V.,Observatory of Herpetology | Sanchez E.,TU Braunschweig | Amat F.,Area dHerpetologia | Hauswaldt J.S.,TU Braunschweig
Salamandra | Year: 2017

We provide an update of the geographical distribution and phylogenetic relationships of mitochondrial haplotype groups of the European common frog, Rana temporaria, one of the most widespread amphibians on Earth. Our data set of 378 newly determined DNA sequences of the cytochrome b gene is combined with 640 previously published sequences, mainly filling a sampling gap in the Spanish Basque country. A haplotype network based on 331 bp provides evidence for a previously unknown, deeply divergent haplogroup in this region, which in the eastern part of its range occurs sympatrically with a widespread group of haplotypes from the Pyrenean mountains, and to the West contacts a haplogroup typically for the central Cantabrian mountains. In contrast, samples from the isolated Montseny massif in the Spanish region of Catalonia are not differentiated from Pyrenean populations. Samples from Scotland cluster in a widespread haplogroup with samples from northern France, western Germany, and Ireland. Our data furthermore suggest that previous records of haplotype sharing of British R. temporaria populations with those from northwestern Spain are probably erroneous. A phylogenetic analysis based on 4,413 bp of mitochondrial DNA confirms the strong divergence of the newly discovered Basque haplogroup and indicates that it might be the sister group of a clade containing all other R. temporaria haplogroups. © 2017 Deutsche Gesellschaft für Herpetologie und Terrarienkunde e.V. (DGHT), Mannheim, Germany.


Andree K.,IRTA Sant Carles de la Rapita | Axtner J.,Leibniz Institute for Zoo and Wildlife Research | Bagley M.J.,U S WEST | Barlow E.J.,UK Center for Ecology and Hydrology | And 142 more authors.
Molecular Ecology Resources | Year: 2010

This article documents the addition of 396 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Anthocidaris crassispina, Aphis glycines, Argyrosomus regius, Astrocaryum sciophilum, Dasypus novemcinctus, Delomys sublineatus, Dermatemys mawii, Fundulus heteroclitus, Homalaspis plana, Jumellea rossii, Khaya senegalensis, Mugil cephalus, Neoceratitis cyanescens, Phalacrocorax aristotelis, Phytophthora infestans, Piper cordulatum, Pterocarpus indicus, Rana dalmatina, Rosa pulverulenta, Saxifraga oppositifolia, Scomber colias, Semecarpus kathalekanensis, Stichopus monotuberculatus, Striga hermonthica, Tarentola boettgeri and Thermophis baileyi. These loci were cross-tested on the following species: Aphis gossypii, Sooretamys angouya, Euryoryzomys russatus, Fundulus notatus, Fundulus olivaceus, Fundulus catenatus, Fundulus majalis, Jumellea fragrans, Jumellea triquetra Jumellea recta, Jumellea stenophylla, Liza richardsonii, Piper marginatum, Piper aequale, Piper darienensis, Piper dilatatum, Rana temporaria, Rana iberica, Rana pyrenaica, Semecarpus anacardium, Semecarpus auriculata, Semecarpus travancorica, Spondias acuminata, Holigarna grahamii, Holigarna beddomii, Mangifera indica, Anacardium occidentale, Tarentola delalandii, Tarentola caboverdianus and Thermophis zhaoermii. © 2010 Blackwell Publishing Ltd.


Sarasola-Puente V.,Observatory of Herpetology | Sarasola-Puente V.,University of the Basque Country | Sarasola-Puente V.,University of Salamanca | Madeira M.J.,University of the Basque Country | And 3 more authors.
Conservation Genetics | Year: 2012

The increasing fragmentation of natural habitats may strongly affect patterns of dispersal and gene flow among populations, and thus alter evolutionary dynamics. We examined genetic variation at twelve microsatellite loci in the Agile frog (Rana dalmatina) from 22 breeding ponds in the Iberian Peninsula, the southwest limit of its range, where populations of this species are severely fragmented and are of conservation concern. We investigated genetic diversity, structure and gene flow within and among populations. Diversity as observed heterozygosities ranged from 0. 257 to 0. 586. The mean number of alleles was 3. 6. Just one population showed a significant F IS value. Four populations show evidence of recent bottlenecks. Strong pattern of structure was observed due to isolation by distance and to landscape structure. The average degree of genetic differentiation among populations was F ST = 0. 185. Three operational conservation units with metapopulation structure were identified. Additionally, there are some other isolated populations. The results reinforce the view that amphibian populations are highly structured even in small geographic areas. The knowledge of genetic structure pattern and gene flow is fundamental information for developing programmes for the preservation of R. dalmatina at the limits of its geographic distribution. © 2011 Springer Science+Business Media B.V.


Sarasola-Puente V.,Observatory of Herpetology | Sarasola-Puente V.,University of the Basque Country | Sarasola-Puente V.,University of Salamanca | Gosa A.,Observatory of Herpetology | And 3 more authors.
Zoology | Year: 2011

The mean age of a population of agile frogs (Rana dalmatina) from the Iberian Peninsula was estimated using mark and recapture and skeletochronology. Life-history parameters, including growth rate, body length, age and size at maturity, sexual dimorphism and longevity, were studied. The regression between age and snout-vent length (SVL) was highly significant in both sexes. Males reached sexual maturity at two years of age, although sometimes they can reach it at only one year of age. The average SVL at maturity was 51.75. mm (standard error (SE) = 0.71; n = 45). Females reached sexual maturity at two years of age with an average SVL of 62.14. mm (SE = 2.20; n = 14). A subset of the female population reached sexual maturity at three years of age. Growth was rapid until sexual maturity was reached. There was an overlap of SVL between different age classes. Growth was continuous, fulfilling the conditions of Von Bertalanffy's model. The growth coefficient (K) was 0.840 in males and 0.625 in females. The maximum SVL was greater in females (73.00. mm) than in males (59.50. mm). Sexual dimorphism was significantly biased towards females in all age classes. The maximum longevity observed was 6 years in females and 8 years in males. Management strategies for agile frogs should take into account factors such as these life-history characteristics. © 2011 Elsevier GmbH.

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