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VANCOUVER, WA, United States

Peres J.C.,University of New Orleans | Rouquette J.L.,University of New Orleans | Miocevic O.,Iowa State University | Warner M.C.,University of New Orleans | And 2 more authors.
Clinical Therapeutics | Year: 2015

Purpose Saliva is a reliable, noninvasive, and cost-effective alternative to biomarkers measured in other biological fluids. Within certain populations, saliva sampling may be difficult because of insufficient saliva flow, which may compromise disease diagnosis or research integrity. Methods to improve flow rates (eg, administering citric acid, chewing gum, or collecting cotton) may compromise biomarker integrity, especially if the methods involve the presence of a collection aid in the oral cavity. Anecsdotal strategies (eg, looking at pictures of food or imagining food) have not been evaluated to date. In this study, we evaluate whether 2 novel collection techniques improve saliva flow or interfere with assay of common biomarkers (ie, cortisol, dehydroepiandrosterone, and testosterone). We evaluate an over-the-counter anhydrous crystalline maltose lozenge intended to increase saliva production for patients with xerostomia long after the lozenge dissolves. We then evaluate whether the smell of freshly cooked bacon stimulates a pavlovian-type reflex. Methods Saliva was collected from 27 healthy young adults (aged 20-34 years; 12 men) on a basal day and a lozenge day, providing 5 samples at 15-minute intervals. Twenty participants then returned for the bacon day condition, providing 2 saliva samples with an interval of 15 minutes between samples. Collection times required to generate 2 mL of saliva across collection strategies were recorded, and then saliva samples were assayed for cortisol, dehydroepiandrosterone, and testosterone. Findings Repeated analysis of variance measures revealed that both the lozenges and bacon significantly decreased collection time compared with the passive drool collection on the basal day. No significant effects were found related to the quantification of cortisol, testosterone, or dehydroepiandrosterone when comparing lozenge or bacon to the basal day. In addition, bivariate correlations revealed that concentrations from time-matched control samples correlated significantly with concentrations from the lozenge and bacon conditions. Implications These results indicate that both the lozenge and smelling bacon improve saliva collection times and that neither technique interferes with salivary hormone concentrations. This study reveals new methods to augment saliva collection strategies. © 2015 Elsevier HS Journals, Inc. Source

Ovchinnikov D.A.,University of Queensland | Cooper M.A.,University of Queensland | Pandit P.,University of Queensland | Coman W.B.,University of Queensland | And 5 more authors.
Translational Oncology | Year: 2012

Head and neck squamous cell carcinoma (HNSCC) accounts for a bulk of the oral and laryngeal cancers, the majority (70%) of which are associated with smoking and excessive drinking, major known risk factors for the development of HNSCC. In contrast to reports that suggest an inverse relationship between smoking and global DNA CpG methylation, hypermethylation of promoters of a number of geneswas detected in saliva collected frompatients with HNSCC. Using a sensitivemethylation-specific polymerase chain reaction (MSP) assay to determine specificmethylation events in the promoters of RASSF1A, DAPK1, and p16 genes, we demonstrate that we can detect tumor presence with an overall accuracy of 81% in the DNA isolated from saliva of patients with HNSCC (n = 143) when compared with the DNA isolated from the saliva of healthy nonsmoker controls (n = 31). The specificity for this MSP panel was 87% and the sensitivity was 80%(with a Fisher exact test P <.0001). In addition, the test panel performed extremely well in the detection of the early stages of HNSCCs, with a sensitivity of 94%and a specificity of 87%, and a high κ concordance value of 0.8, indicating an excellent overall agreement between the presence of HNSCC and a positive MSP panel result. In conclusion, we demonstrate that the promoter methylation of RASSF1A, DAPK1, andp16 MSP panel is useful in detecting hypermethylation events in a noninvasive manner in patients with HNSCC. © 2012 Neoplasia Press, Inc. Source

Oasis Diagnostics Corporation | Date: 2015-11-24

Medical specimen collection device in the nature of absorbent pads, plastic tubes, absorbent pad holders for the collection of cervicovaginal cells.

An apparatus for generating and collecting saliva samples containing enhanced cell concentrations includes a mouthpiece having a mouth contact portion and a flow channel which may include a tapered basin, a conduit in fluid communication with the flow channel, and one or more collectors couplable to the conduit. The mouth contact portion and basin may be textured to enhance cell removal. The mouth contact portion may include a plurality of internal and external channels disposed around the flow channel. Filters may be included between the flow channel and the collectors. A closure member is provided.

Oasis Diagnostics Corporation | Date: 2014-03-03

Medical specimen collection device in the nature of absorbent pads, plastic tubes, absorbent pad holders for the collection of cervicovaginal cells.

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