NVH Medicinal

Dijon, France

NVH Medicinal

Dijon, France
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Maurizi L.,Laboratory Interdisciplinaire Carnot de Bourgogne | Maurizi L.,Ecole Polytechnique Federale de Lausanne | Papa A.-L.,Laboratory Interdisciplinaire Carnot de Bourgogne | Papa A.-L.,Wyss Institute for Biologically Inspired Engineering | And 5 more authors.
Journal of Biomedical Nanotechnology | Year: 2015

The aim of this study was to investigate the influence of the surface charge and coating of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) on their in vitro and in vivo behaviors. Neutral and negatively-charged PEG-based SPIONs were synthesized and compared to Resovist®, a carboxydextran-based SPION currently used in clinics. Their cytotoxicity, cell internalization, and potential as contrast agents for magnetic resonance imaging were assessed. Neutral pegylated SPIONs were internalized less readily by the reticuloendothelial system and showed a lower uptake by the liver, compared to negatively-charged SPIONs (with carboxydextran and PEG). These results suggested that the charge of functionalized SPIONs was more relevant for their biological interactions than the nature of their coating.

The present invention relates to an injectable preparation comprising particles or fibrils with length less than 10 m and at least one pharmaceutically acceptable vehicle or excipient. The particles or fibrils comprise proteins or peptides inducing adhesion and activation of the platelets, or even aggregation thereof. The preparation is useful for treating haemorrhages.

Nvh Medicinal and University of Burgundy | Date: 2013-10-18

A method for diagnosing von Willebrand disease and novel polypeptides which bind to von Willebrand factor.

Papa A.-L.,University of Burgundy | Maurizi L.,University of Burgundy | Vandroux D.,NVH Medicinal | Walker P.,University of Burgundy | Millot N.,University of Burgundy
Journal of Physical Chemistry C | Year: 2011

For the first time, titanate nanotubes (TiONts) coated with USPIO (ultrasmall superparamagnetic iron oxide) without polymer functionalization and directly obtained during TiONts formation is reported. The coating of these tubes was performed directly during the TiONts hydrothermal synthesis. After this treatment, in strongly basic conditions, USPIO particles kept their structure and magnetic properties while their size distribution slightly increased. By coupling zeta-potential measurements and TEM observations at different pH it appeared that interactions between nanotubes and USPIO were not electrostatic. This study presents the new design of a titanate needle-shaped nanocomposite capable of being detected by MRI for future biomedical applications. © 2011 American Chemical Society.

de Maistre E.,University Hospital | Volot F.,University Hospital | Mourey G.,Etablissement Francais du Sang | Aho L.S.,University Hospital | And 9 more authors.
Thrombosis and Haemostasis | Year: 2014

The ristocetin cofactor activity assay (VWF:RCo) is the reference method for assessing von Willebrand factor (VWF) activity but remains difficult to perform, and the coefficient of variation of the method is high (about 20–30%). This study evaluated and compared the performance for measuring the VWF activity of two newly commercialised assays [VWF:Ac Innovance (VWF:Ac) and VWF:RCo Acustar (VWF:RCo Acu)] with the reference VWF:RCo aggregation in 123 pathological plasma samples. The correlation and concordance between both new tests (VWF:RCo-Acu and VWF:Ac) and the reference VWF:RCo were good. The results of the VWF activity to VWF antigen ratio were also comparable whatever the method for the classification of VWF deficiency in all patients. Our results showed that both new tests could replace the “gold standard” VWF:RCo in aggregometry with several benefits: They are fully automated, easier and faster to perform, better adapted to emergency situations if necessary. © Schattauer 2014.

Jacquir S.,CNRS Laboratory of Electronics Informatics and Images | Binczak S.,CNRS Laboratory of Electronics Informatics and Images | Xu B.,CNRS Laboratory of Electronics Informatics and Images | Laurent G.,CNRS Laboratory of Electronics Informatics and Images | And 3 more authors.
International Journal of Bifurcation and Chaos | Year: 2011

During cardiac arrhythmia, functional reentries may take the form of spiral waves. The purpose of this study was to induce spiral waves by an electrical stimulation of cultured neonatal rat cardiomyocytes using a microelectrode arrays technology. In basal conditions, cardiac muscle cells in monolayer culture displayed a planar wavefront propagation. External electrical impulse trains induced severe arrhythmia and spiral waves appeared. This in vitro generation of spiral wave opens a new way to test the anti-arrhythmic drugs and for strategies at microscopically scale. © 2011 World Scientific Publishing Company.

Papa A.-L.,Laboratory Interdisciplinaire Carnot de Bourgogne | Dumont L.,NVH Medicinal | Vandroux D.,NVH Medicinal | Millot N.,Laboratory Interdisciplinaire Carnot de Bourgogne
Nanotoxicology | Year: 2013

Actively contractile cardiomyocyte (CM) monolayer represents an interesting tool to study both cardiac diseases and injuries. However, this model is poorly transfectable with conventional agents. Consequently, there is a need to develop new carriers that could overcome this problem. Titanate nanotubes (TiONts) could be a potential candidate due to possibly higher cell uptake as a direct consequence of their shape. On the basis of this rationale, TiONts were assessed for their cytotoxicity and internalization pathways. Cytotoxicity was assessed for TiONts either functionalized with PEI or unfunctionalized and its spherical counterpart P25 TiO2. No cytotoxic effect was observed under TiONts, TiONts-PEI1800 and P25 TiO2 exposed conditions. The tubular morphology was found to be an important parameter promoting internalization while reversing the charge was assessed as non-additional. Internalization was found to occur by endocytosis and diffusion through the membrane. A preliminary transfection study indicated the potential of TiONts as a nanocarrier. © 2013 Informa UK, Ltd.

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