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Yaguiyan-Colliard L.,National Veterinary School of Alfort | Daumas C.,Nutrition and Endocrinology Unit | Nguyen P.,Nutrition and Endocrinology Unit | Grandjean D.,National Veterinary School of Alfort | And 3 more authors.
BMC Research Notes | Year: 2015

Abstract Background: Equations based on single-frequency bioelectrical impedance analysis at 50 kHz for determination of total body water content (TBW) have been previously validated in healthy non-sedated beagle dogs. We investigated whether these equations are predictive of TBW in various canine breeds by comparing the results of these equations with TBW values evaluated directly by deuterium oxide (D2O) dilution. Methods: Total body water content of 13 healthy adult pet dogs of various breeds was determined directly using D2O dilution and indirectly using previous equations based on values obtained with a portable bioelectric impedance device. Paired Student's t-tests were used to compare TBW obtained by single-frequency bioelectrical impedance analysis and D2O dilution. A p-value of <0.05 was considered statistically significant for all analyses. Results: Significant differences were observed between TBW determined by the reference method and the values obtained with both predictive equations. Conclusions: The proposed equations including single-frequency bioelectrical impedance analysis parameters validated at 50 kHz in healthy adult beagles need to be modified including morphological parameters such as body size and shape in a first approach. As in humans, morphological-specific equations have to be developed and validated. © 2015 Yaguiyan-Colliard et al.

PubMed | UPSP 5304, Mycology Unit and Nutrition and Endocrinology Unit
Type: Journal Article | Journal: Veterinary dermatology | Year: 2016

The diagnosis of canine atopic dermatitis (CAD) remains challenging due to the lack of a simple biomarker or metabolic profile. In human medicine, Fourier transform infrared spectroscopy (FTIR) is an analytical technique used for several diseases. It requires a small amount of sample and allows the identification of structural moieties of biomolecules on the basis of their infrared absorption, with limited sample pretreatment.The aim of the study was to evaluate the diagnostic value of FTIR.Three groups were tested: 21 dogs with non food-induced CAD (NFICAD), 16 dogs with inflammatory conditions of various origins but without allergic dermatoses (OD) and 10 healthy dogs (H).Peripheral blood was collected and spectra were acquired with a FTIR spectrophotometer. A principal component analysis (PCA) was performed on the full wavenumber spectra (4000-600/cm), followed by a Fisher discriminant analysis (DA) to assess the differences between the three groups.The PCA followed by the DA of whole spectra showed significant differences between the three groups. These results suggest that by using the FTIR method, dogs with NFICAD can be differentiated from healthy dogs and dogs with nonallergic inflammation. There was no overlap between the spectral data of the three groups indicating that NFICAD dogs were correctly segregated from the H and OD groups.A study on a larger cohort including common pruritic skin diseases is necessary to confirm these initial results and the relevance of this diagnostic technique.

Amato C.,Nutrition and Endocrinology Unit | Martin L.,Nutrition and Endocrinology Unit | Siliart B.,Nutrition and Endocrinology Unit | Nguyen P.,Nutrition and Endocrinology Unit | Dumon H.,Nutrition and Endocrinology Unit
EAAP Scientific Series | Year: 2010

Exercise-induced fatigue is associated with various changes in physiological parameters. The aim of the present study was to determine the relationship between some biological parameters and body weight loss, energy intake and workload. Twenty-one healthy show horses of Andalusian or Lusitanian breeds were included in the study. All the horses were weighed twice monthly and body condition score was determined monthly using INRA's method (scale 0 to 5). Blood samples were collected monthly at rest for standard biochemical assays. Workload was determined according of heart rate and correlations between zootechnical parameters and biological parameters were calculated. Variations in the biological parameters were determined with a linear mixed effects model (P<0.05). A significant decrease in body weight occurred during follow-up despite the increased energy intake. The variations in body weight, energy intake and workload were significantly correlated with creatinine, hematocrit, fibrinogen, aspartate aminotransferase, gammaglutamyl transferase, glucose, lactate, cholesterol, HDL-cholesterol, sodium, potassium, magnesium, chloride, calcium and phosphates at rest parameters. A decrease in body weight despite an increase in energy intake may be useful for detecting chronic fatigue in show horses and this may be due to excessive catabolic effect of some hormones and cytokines stimulated by workload and stress.

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