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Nam D.-E.,Kyung Hee University | Lee M.-J.,Nutriplan Co. | Kang N.,Nutriplan Co. | Park G.,Suheung Capsule R and nter | Lee J.,Kyung Hee University
Journal of the Korean Society of Food Science and Nutrition | Year: 2012

The inhibitory effects of rose hip (Rosa canina L.) water extracts from two different manufactures on osteoarthritis was comparatively investigated in primary cultures of rat cartilage cells. To identify the effects of rose hip extracts against H2O2 (300 μM, 2 hr) treatment, cell survival was measured by the 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell survival increased by rose hip extracts in the range of 100 to 600 μg/mL of H2O2 treatment. To determine the anti-inflammatory effects of rose hip extracts, tumor necrosis factor alpha (TNF-α), nitric oxide (NO), and Cox-2 expression were measured after lipopolysaccharide (LPS) activation. TNF-α level with rose hip extract treatment was decreased by 27.4% and 31.9% at 600 μg/mL of H2O2 treatment. Nitric oxide was inhibited by rose hip extract at 100~600 μg/mL of H2O2 treatment in a dose-dependent manner. In addition, Cox-2 protein expression was dose-dependently decreased while Cox-1 had no change in expression level. The severity of osteoarthritis is controlled by a balance between anabolic and catobolic factors in an articulation, therefore the expression of these factors plays a critical role in preventing osteoarthritis. In measuring anabolic factors, the genetic expression of collagen type I increased with rose hip treatment, while the genetic expression of collagen II did not change. In addition, the genetic expression of aggrecan (proteoglycan core protein) was significantly increased. while the genetic expression of matrix metalloproteinase (MMP) 3, 7 and 13, known catabolic factors, was significantly inhibited by treatment with rose hip extract. The expression of MMP13 was especially highly influenced. In conclusion, rose hip water extracts show inhibitory effects on cell death by H2O2 mediated oxidative stress, which is related to inhibitory effects on inflammation due to TNF-α, NO, and Cox-2. The ability of rose hip extracts to ameliorate inflammation in primary cultures of cartilage cells seems to associate with an increased genetic expression of specific anabolic factors, collagen type I and aggrecan, and a decreased expression of catabolic factors, MMPs (3, 7, and 13). However, there were no significant differences between rose hip extracts from the two manufacturers. Source


Kim O.K.,Kyung Hee University | Nam D.-E.,Kyung Hee University | Lee M.-J.,Nutriplan Co. | Kang N.,Nutriplan Co. | And 2 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2014

In this study, we investigated the protective effects of green tea seed extract (GSE) against UVB-induced skin damage in human skin fibroblasts. GSE was first analyzed for antioxidant activity using 1,1-diphenyl-2picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging assays. Treatment of UV-irradiated fibroblast with GSE at 10~50 μg/mL significantly increased DPPH and ABTS radical scavenging activities in a dose-dependent manner. GSE treatment inhibited matrix metalloproteinase (MMP-1, MMP-3, and MMP-9) expression and MMP-1 secretion caused by UVB irradiation. Moreover, treatment with GSE significantly increased type-1 collagen expression and production. We next examined levels of antioxidative enzymes (SOD, catalase, and GPx). Reduced antioxidative enzyme activities caused by UVB irradiation were recovered by treatment with GSE at 30 μg/mL and 50 μg/mL. In conclusion, these results show that GSE has protective effects against UVB-induced skin damage in human skin fibroblasts by regulating antioxidative defense systems and MMP expression. Source


Lim J.-Y.,Yonsei University | Kim O.-K.,Kyung Hee University | Lee J.,Kyung Hee University | Lee M.-J.,Nutriplan Co. | And 2 more authors.
Nutrition Research and Practice | Year: 2014

Background/Objectives: Ultraviolet B (UVB) irradiation on skin can induce production of reactive oxygen species (ROS), which cause expression of matrix metalloproteinases (MMPs) and collagen degradation. Thus, chronic exposure of skin to UVB irradiation leads to histological changes consistent with aging, such as wrinkling, abnormal pigmentation, and loss of elasticity. We investigated the protective effect of the standardized green tea seed extract (GSE) on UVB-induced skin photoaging in hairless mice. Materials/Methods: Skin photoaging was induced by UVB irradiation on the back of Skh-1 hairless mice three times per week and UVB irradiation was performed for 10 weeks. Mice were divided into six groups; normal control, UVB irradiated control group, positive control (UVB + dietary supplement of vitamin C 100 mg/kg), GSE 10 mg/kg (UVB + dietary supplement of GSE 10 mg/kg), GSE 100 mg/kg (UVB + dietary supplement of GSE 100 mg/kg), and GSE 200 mg/kg (UVB + dietary supplement of GSE 200 mg/kg). RESULTS: The dietary supplement GSE attenuated UVB irradiation-induced wrinkle formation and the decrease in density of dermal collagen fiber. In addition, results of the antioxidant analysis showed that GSE induced a significant increase in antioxidant enzyme activity compared with the UVB irradiation control group. Dietary supplementation with GSE 200 mg/kg resulted in a significant decrease in expression of MMP-1, MMP-3, and MMP-9 and an increase in expression of TIMP and type-1 collagen. Conclusions: Findings of this study suggest that dietary supplement GSE could be useful in attenuation of UVB irradiation-induced skin photoaging and wrinkle formation due to regulation of antioxidant defense systems and MMPs expression. © 2014 The Korean Nutrition Society and the Korean Society of Community Nutrition. Source


Osaki K.,Bizen | Arakawa T.,Bizen | Kim B.,Kyungil University | Lee M.,Nutriplan Co. | And 2 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2016

This study was conducted to investigate the protective effects of water extract from Crassostrea gigas (CGW) against ethanol-induced hepatic toxicity in rats. Seventy-two male Wistar rats (6-week-old) were divided into six groups of 12 animals each: control group (1 mL saline/d), ethanol-treated group, positive control group (ethanol+ Hovenia dulcis Thunb extract), CGWL group (ethanol+low dosage of CGW), CGWM group (ethanol+medium dosage of CGW), and CGWH group (ethanol+high dosage of CGW). All groups except the control group received ethanol (40% ethanol 5 g/kg) orally. CGW administration with ethanol resulted in prevention of ethanol-induced hepatotoxicity by increasing levels of serum alanine aminotransferase and γ-glutamyltransferase. CGW supplementation significantly reduced formation of malonaldehyde and inhibited reduction of hepatic glutathione and peroxidase levels, as compared with the ethanol-administration group. Further, CGW suppressed expression of CYP2E1, which was elevated by ethanol administration. Consequently, our results indicate that Crassostrea gigas may exert hepatoprotective effects against alcohol-induced hepatocyte injury by intensifying the anti-oxidative defense system. © 2016, Korean Society of Food Science and Nutrition. All rights reserved. Source


Jeong C.,Nutriplan Co. | Bae D.,Jeollanamdo Institute of Natural Resources Research JINR | Lim H.,Korea Health Supplements Institute KHSI | Lee M.,Nutriplan Co. | And 2 more authors.
Animal Cells and Systems | Year: 2015

In this study, we investigated the relationship between anti-inflammatory mechanisms and the combined ameliorating effects of rose hip powder (RHP) and green tea seed extract (GTSE) in a monosodium iodoacetate (MIA)-induced osteoarthritis (OA) animal model. We confirmed the individual effects of RHP (500 mg/kg bw) and GTSE (50 mg/kg bw) in the OA model. Treatment with the mixture of RHP and GTSE (Mix) resulted in significantly enhanced stance and propulsion times compared to treatment with RHP or GTSE alone. To examine the combined effects of RHP and GTSE in vivo, pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), were measured. The administration of Mix significantly reduced the expression of pro-inflammatory cytokines. Additionally, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expressions were attenuated to a greater extent after the administration of Mix compared to the other treatments. Furthermore, we evaluated the anti-osteoarthritic effects of RHP, GTSE, and Mix on articular cartilage damage using micro-computed tomography (micro-CT) in OA in rats. After three weeks of treatment, we observed that the administration of RHP, GTSE, and Mix protected against bone destruction and reduced the number of erosion lacunae, but there was no statistical difference among RHP, GTSE, and Mix. Although additional research is warranted, our results suggest that the biological effects of GTSE were enhanced by RHP supplementation to include anti-inflammatory effects, with the potential ability of offering a benefit to OA patients. © 2014, Korean Society for Integrative Biology. Source

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