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Murcia, Spain

Baldasquin-Caceres B.,University of Murcia | Gomez-Garcia F.J.,University of Murcia | Lopez-Jornet P.,University of Murcia | Castillo-Sanchez J.,Nutrafur SA | Vicente-Ortega V.,University of Murcia
Archives of Oral Biology

Objective To evaluate the chemopreventive potential of phenolic compounds - potassium apigenin, cocoa, catechins, eriocitrin and rosmarinic acid in oral carcinogenesis induced in hamsters by means of the topical application of 7,12-dimethylbenz(a)anthracene(DMBA). Study design An experimental study at the University of Murcia. Methods 50 male Syrian hamsters (Mesocricetus auratus) were divided into five groups of ten: Group I (control group): 0.5% DMBA; Group II: 0.5% DMBA + 1.1 mg/15 ml potassium apigenin; Group III: 05% DMBA + 2.5 mg/15 ml cocoa catechins; Group IV: 0.5% DMBA + 6 mg/15 ml eriocitrin; Group V: 0.5% DMBA + 1.3 mg/15 ml rosmarinic acid. The flavonoids were administered orally. All the animals were sacrificed after 12 weeks. Macroscopic, microscopic and immunohistochemical (PCNA and p53) analyses of the lesions were performed. Results All the groups treated with phenolic compounds showed lower incidences of tumour, greater differentiation and lower scores in the tumour invasion front grading system in comparison with the control group. Potassium apigenin and rosmarinic acid achieved the best results, the former considerably reduced the carcinoma tumour volumes developed and both significantly reduced the intensity and aggression of the tumours. Immunoexpression of PCNA and p53 were significantly altered during DMBA-induced oral carcinogenesis. Conclusions Animals treated with phenolic compounds, particularly potassium apigenin and rosmarinic acid, showed a lower incidence of tumours. © 2014 Elsevier Ltd. Source

Jordan M.J.,Murcia Institute of Agri Food Research and Development IMIDA | Castillo J.,Nutrafur SA | Castillo J.,University of Murcia | Banon S.,University of Murcia | And 2 more authors.
Food Chemistry

The aim of the present work was to evaluate whether the relation between the concentrations of the two major diterpenes present in two typified rosemary extracts affects their levels of deposition and antioxidant capacity in different lamb tissues. The composition of the extracts expressed as percentage of weight/weight was 1:1 (14-16)% and 2:1 (25-11)% (carnosic acid-carnosol), respectively. Thirty weaned lambs were assigned randomly to three homogeneous groups. One group was fed a basal diet as a control and the diets of the other two were enriched with rosemary extracts 1:1 and 2:1, respectively. HPLC-ESI-MS/TOF identified a metabolite (C19H22O 3) described for the first time in lamb tissues, along with carnosol, carnosic acid, rosmanol and carnosol-p-quinone. The results obtained corroborate the importance of the presence of carnosol in the dietary administration of rosemary extract as a way of improving the stability of the diterpene fraction during feed manufacturing and the level of deposition and antioxidant efficacy of diterpenes after ruminal fermentation. © 2013 Elsevier Ltd. All rights reserved. Source

Bayoa J.,Technical University of Cartagena | Estebana G.,Technical University of Cartagena | Castillob J.,Nutrafur SA
Environmental Technology (United Kingdom)

This paper describes the use of native and protonated grapefruit biomass, a by-product of the food industry, as an effective and low-cost biosorbent for cadmium removal from aqueous solutions. The biomass composition was analysed by highperformance liquid chromatography, scanning electron microscopy coupled with energy-dispersive X-ray analysis and Fourier transform infrared spectroscopy, showing that hydroxyl and carboxylic groups were the main functional groups implicated in Cd(II) biosorption. The effect of different parameters affecting the biosorption process were studied. The optimum removal of cadmium ions was at pH 4.5. Elution of alkaline-earth ions proved to be related with cadmium uptake, aiming for an ionexchange mechanism. Protonated biomass showed higher adsorption affinity, binding strength and irreversibility for cadmium than native grapefruit, although the optimum metal uptake and high reaction rate was for the native form of grapefruit. Biosorption experimental data fitted Freundlich > Langmuir > Temkin equilibrium adsorption models. Data for both types of biomass were better fitted by a pseudo-second-order kinetic model, with an excellent correlation between calculated and experimental values. Because of these experimental results, and taking into account that both types of biomass displayed an exothermic and spontaneous physical adsorption process, native grapefruit can be proposed in further experiments as a cheap, effective, low-cost and environmentally friendly natural sorbent for the removal of cadmium fromindustrial wastewater effluents, avoiding chemical pretreatment before its use. © 2012 Taylor & Francis. Source

Guerrero L.,Rovira i Virgili University | Guerrero L.,ALPINA S.A. | Castillo J.,Nutrafur SA | Quinones M.,Rovira i Virgili University | And 8 more authors.

Previous studies have demonstrated that certain flavonoids can have an inhibitory effect on angiotensin-converting enzyme (ACE) activity, which plays a key role in the regulation of arterial blood pressure. In the present study, 17 flavonoids belonging to five structural subtypes were evaluated in vitro for their ability to inhibit ACE in order to establish the structural basis of their bioactivity. The ACE inhibitory (ACEI) activity of these 17 flavonoids was determined by fluorimetric method at two concentrations (500 μM and 100 μM). Their inhibitory potencies ranged from 17 to 95% at 500 μM and from 0 to 57% at 100 μM. In both cases, the highest ACEI activity was obtained for luteolin. Following the determination of ACEI activity, the flavonoids with higher ACEI activity (i.e., ACEI >60% at 500 μM) were selected for further IC50 determination. The IC50 values for luteolin, quercetin, rutin, kaempferol, rhoifolin and apigenin K were 23, 43, 64, 178, 183 and 196 μM, respectively. Our results suggest that flavonoids are an excellent source of functional antihypertensive products. Furthermore, our structure-activity relationship studies show that the combination of sub-structures on the flavonoid skeleton that increase ACEI activity is made up of the following elements: (a) the catechol group in the B-ring, (b) the double bond between C2 and C3 at the C-ring, and (c) the cetone group in C4 at the C-ring. Protein-ligand docking studies are used to understand the molecular basis for these results. © 2012 Guerrero et al. Source

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La privilegiada situacin de nuestras instalaciones, en Murcia, al sureste de Espaa, nos permite disponer y aprovechar grandes cantidades de diversas especies vegetales tpicamente mediterrneas, alcanzando as, una perfecta y respetuosa integracin con nuestro entorno ms cercano. La cuidada seleccin de nuestras materias primas, mediante los ms estrictos controles de calidad, es igualmente un factor clave de nuestro xito ...

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