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Lin H.-H.,Nugen Bioscience Taiwan Co. | Yin L.-J.,National Kaohsiung Marine University
Journal of Marine Science and Technology | Year: 2010

A feather-degrading bacterium with high keratinase activity was isolated and identified as Bacillus licheniformis YJ4. After 72 h incubation in a medium (0.5% feather meal, 0.05% NH4Cl and NaCl, 0.04% K2HPO 4, 0.03% KH2PO4, 0.01% MgCl2 and yeast extract, 0.1% rice husk) at 37°C, 2 keratinases (keratinase I and II) were purified to electrophoretical homogeneity by CM sepharose and Sephadex G-75 chromatographs. They were with molecular masses (M) of 35.5 and 32.8 kDa, isoelectric point (pI) of 6.63 and 6.50, respectively, and stable at pH 6.0-10.0 and 10-50°C. The optimal pH and temperature were similar, at 9.0 and 60°C, respectively. According to the effect of metal, inhibitor and reducing agent, and previous studies, the purified keratinases I and II were considered to be cysteine and serine proteases, respectively.


Yin L.-J.,National Kaohsiung Marine University | Tai H.-M.,Providence University | Tai H.-M.,Nugen Bioscience Taiwan Co. | Jiang S.-T.,Providence University | Jiang S.-T.,National Taiwan Ocean University
Journal of Agricultural and Food Chemistry | Year: 2012

Locust bean gum (LBG) was employed to screen mannanase-producing bacteria. The bacterium with highest mannanase ability was identified as Paenibacillus cookii. It revealed highest activity (6.67 U/mL) when cultivated in 0.1% LBG with 1.5% soytone and 0.5% tryptone after 4 days incubation at 27 °C. Its mannanase was purified to electrophoretical homogeneity after DEAE-Sepharose and Sephacryl S-100 separation. The purified mannanase, with an N-terminus of GLFGINAY, had pH and temperature optimum at 5.0 and 50 °C, respectively, and was stable at pH 5.0-7.0, ≥50 °C. It was strongly activated by β-mercaptoethanol, dithiothreitol, cysteine, and glutathione, but inhibited by Hg2+, Cu2+, Zn2+, Fe3+, PMSF, iodoacetic acid, and EDTA. According to substrate specificity study, the purified mannanase had high specificity to LBG and konjac. © 2012 American Chemical Society.


Ciou J.-Y.,Ming Dao University | Lin H.-H.,Nugen Bioscience Taiwan Co. | Chiang P.-Y.,National Chung Hsing University | Wang C.-C.,Providence University | Charles A.L.,National Pingtung University of Science and Technology
Food Chemistry | Year: 2011

The mechanism of browning involving enzymatic browning was investigated in the pericarp of water caltrop, an Asian vegetable popular for its taste and medicinal properties. Polyphenol oxidase (PPO) and peroxidase (POD) activities were determined in pericarp at various times and temperatures. Water caltrop consisted of 44.22% moisture content, 37.23% crude fibre, and 2.63% crude protein. PPO and POD activities dropped from 62 and 38 units/g sample, respectively, as water temperature was increased from 30 to 80°C. Optimum pH and temperature for PPO activity was at pH 5.0, 25-45°C, and POD activity peaked at 60°C. High PPO and POD activities at 40-50°C resulted in degradation of phenolic compounds, which led to increased aggregation of browning pigments and discolouration (lower L-values) of the pericarp. Enzymatic browning was determined as the major factor in the browning discolouration of heat-treated water caltrop pericarp. © 2011 Elsevier Ltd. All rights reserved.


Yin L.-J.,National Kaohsiung Marine University | Lin H.-H.,Nugen Bioscience Taiwan Co. | Jiang S.-T.,National Taiwan Ocean University | Jiang S.-T.,Providence University
Journal of Agricultural and Food Chemistry | Year: 2010

Fibrinolytic enzyme activity was observed during cultivation of Bacillus subtilis YJ1 in a medium containing 1% skim milk, 1% rice husk, 0.5% NaCl, and 0.25% glucose. It was purified to electrophoretical homogeneity after CM-sepharose FF chromatography. The specific activity and yield were 1791.9 FU/mg and 9.5%, respectively. This purified fibrinolytic enzyme had M of 27.5 kDa, optimal temperature and pH at 50 °C and 8.5, respectively. It was stable at pH 6.0-10.0 and 10-40 °C and inhibited by Fe3+, Hg 2+, Cu2+, Zn2+, and PMSF. Compared the N terminal of amino acids and full DNA sequence with those in NCBI, it was considered to be a nattokinase. © 2010 American Chemical Society.


Patent
Nugen Bioscience Taiwan Co. | Date: 2013-03-15

The present invention provides a mineral-peptide chelate comprising a peptide consisting of 218 amino acids and a mineral chelated to the peptide, wherein the peptide can be a hydrolysate obtained by hydrolyzing soybean or other protein materials with proteases, or a product obtained by hydrolyzing soybean or other protein material with proteases and fermentation. The mineral-peptide chelate of the present invention may further comprise a carrier which covers the peptide and the mineral which is chelated to the peptide.

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