Time filter

Source Type

Srihari T.,NTR University of Health Sciences | Karthikesan K.,Annamalai University | Ashokkumar N.,Annamalai University | Satyanarayana U.,Dr Pinnamaneni Siddhartha Institute Of Medical Science
Journal of Functional Foods | Year: 2013

Kombucha is a fermented tea beverage produced by fermenting sugared black tea with kombucha mat (consists of bacteria and yeast strains). Diabetes mellitus is group of metabolic disorders characterized by hyperglycaemia resulting from defects in insulin secretion, action or both. The present study was aimed to delineate the antihyperglycaemic effect of lyophilized extract of kombucha in streptozotocin-induced experimental rats. After the experimental period of 45days we observed that kombucha supplementation with 6mg/kg bw significantly decreased glycosylated haemoglobin (HbA1c) and increased the levels of plasma insulin, haemoglobin and tissue glycogen which was decreased up on streptozotocin (STZ) treatment and also significantly reversed the altered activities of gluconeogenic enzymes such as glucose-6-phosphatase, fructose-1,6-bisphosphatase and glycolytic enzymes such as hexokinase in the tissues of experimental rats. Thus, our results substantiate that kombucha found to exert hypoglycaemic effect in STZ-induced diabetic rats. These findings suggest that kombucha may be considered as a potential functional food candidate for future applications as functional food supplement for the treatment and prevention of diabetes. © 2013 Elsevier Ltd. Source

Rao G.S.,NTR University of Health Sciences | Vijay Kumar M.,Veterinary College
Indian Journal of Pharmacology | Year: 2012

Objective: To assess pharmacokinetic interaction of garlic with atorvastatin in dyslipidemic rats. Materials and Methods: Sprague Dawley rats with induced dyslipidemia were divided into five groups of eight rats each. Group 1 was given atorvastatin (10 mg/kg body weight (b.wt) orally), group 2 was given atorvastatin (10 mg/kg b.wt orally)+garlic (1% w/w in feed), group 3 was maintained on atorvastatin (5 mg/kg b.wt orally)+garlic (0.5% w/w in feed), group 4 was maintained on atorvastatin (7.5 mg/kg b.wt orally)+garlic (0.25% w/w in feed), and group 5 was maintained on atorvastatin (2.5 mg/kg b.wt orally)+garlic (0.75% w/w in feed) for 12 weeks. Blood samples were collected at predetermined time intervals for kinetic analysis after the first and last oral dosing of atorvastatin for single and multiple dose studies, respectively. Plasma samples were assayed for atorvastatin concentration by High-Performance Liquid Chromatography (HPLC) and then the concentration-time data were analyzed. Results: Maximum observed plasma concentration (C max), half-life, Area Under Plasma Concentration Time Curve (AUC), and Mean Resident Time (MRT) were significantly (P<0.05) increased during multiple dose kinetic study and elimination rate constant was significantly (P<0.05) decreased in comparison with their respective single-dose values, while there was no significant difference in time to achieve maximum concentration (t max) in all groups during both phases of the study. The highest values for kinetic parameters were observed in group 2 with correspondingly low activity of Cytochrome P 450 (CYP 450). Conclusion: The study revealed higher values [C max, AUC, Area Under The Moment Curve (AUMC), MRT, and half-life] of atorvastatin in garlic-treated groups. Source

Bindu Kiranmayi C.,NTR University of Health Sciences
Veterinary World | Year: 2010

Escherichia coli O157:H7 is an emerging public health concern in most countries of the world. E. coli O157:H7 was known to be a human pathogen for nearly 24 years. EHEC O157 infection is estimated to be the fourth most costly food borne disease in Canada and USA, not counting the cost of possible litigation. E. coli O157:H7 and Salmonella are the leading causes of produce related outbreaks, accounting for 20 and 30% respectively. The authority of the Federal Meat Inspection Act, FSIS (Food Safety and Inspection Service) declared Escherichia coli O157:H7, an adulterant in raw ground beef and enforced "zero tolerance" (USDA-FSIS, 17 December 1998). Because of the severity of these illnesses and the apparent low infective dose (less than 10 cells), Escherichia coli O157:H7 is considered one of the most serious of known food borne pathogens. Escherichia coli O157:H7 is mainly pathogenic to human but in cattle and other animals, it did not induce any clinical disease except diarrhea. So, these animals act as carriers to Escherichia coli O157:H7. The majority transmission is through eating of undercooked contaminated ground meat and consumption of raw milk, raw vegetables, fruits contaminated by water, cheese, curd and also through consumption of sprouts, lettuce and juice. The conventional isolation procedure includes growth in enrichment broth like modified EC (E. coli) broth or modified tryptic soy broth (mTSB) Since the infection primarily occurs via faeco-oral route, the preventive measures include food hygiene measures like proper cooking of meat, consumption of pasteurized milk, washing fruits and vegetables especially those to be eaten raw and drinking chlorine treated water and personnel hygiene measures like washing hands after toilet visits. Source

Munshi A.,Central University of Punjab | Das S.,Osmania University | Das S.,NTR University of Health Sciences | Kaul S.,Nizams Institute of Medical Sciences
Gene | Year: 2015

Stroke is a global health problem and a leading cause of disability worldwide. There have been numerable studies undertaking research on different aspects of ischaemic stroke employing various epidemiological, clinical and molecular parameters. Nevertheless ischaemic stroke being a complex disorder with different subtypes demands equal attention towards its subtypes too. Since there has been enough evidence that disposition to certain subtype is genetically determined and there is a distinct mechanism that influences its development, association studies should focus on subtypes simultaneously while studying specific genes. Data from such studies will thus provide better and intricate findings with regard to heterogenous ischaemic stroke. In the present review we discuss the genes studied by our group over a period of seven years in association with stroke subtypes in a South Indian population and correlate the findings with similar genetic studies from other populations so as to provide an overview of various genes involved in the pathogenesis of ischaemic stroke subtypes. © 2014 Elsevier B.V. Source

Sharma V.,Osmania University | Sharma V.,NTR University of Health Sciences | Kaul S.,Nizams Institute of Medical Sciences | Al-Hazzani A.,King Saud University | And 3 more authors.
Journal of Thrombosis and Thrombolysis | Year: 2013

Aspirin is the most commonly used antiplatelet drug for treatment of a serious vascular event, most notably stroke and myocardial infarction. However, despite the demonstrated benefit of aspirin, significant fraction of aspirin-treated patients may be resistant to the antiplatelet effects of the drug. The possible mechanisms of aspirin resistance (AR) are multifactorial. A genetic basis for AR has been suggested to exist. Therefore, the present study was taken up to investigate the role of -765G/C polymorphism (rs20417) in the cyclooxygenase-2 (COX-2) gene with AR in stroke patients. Four hundred and fifty stroke patients and four hundred and forty age and sex matched healthy controls were involved in the study. Baseline clinical data were collected and follow-up telephone interviews were conducted with patients at 3 months post event to determine stroke outcome using Modified Rankin Scale. Blood samples were collected and genotypes determined by polymerase chain reaction-restriction digestion technique. The association between the genotypes and outcome was evaluated by stepwise multiple logistic regression analysis. The COX-2 CC and GC genotype showed a significant association with bad outcome. Therefore, the carriers of C allele of COX-2 -765G/C polymorphism are more prone to AR in comparison with non-carriers. These results support a potential role of -765G/C COX-2 gene polymorphism with AR in ischemic stroke patients. © 2012 Springer Science+Business Media, LLC. Source

Discover hidden collaborations