Nagaland, India
Nagaland, India

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das K.C.,NRC on Mithun
Livestock Research for Rural Development | Year: 2012

The performance of growing cross bred pigs was evaluated when maize was replaced with tapioca (Manihot esculenta) root meal at different levels. Twenty four crossbred piglets (Large black X Nagaland local) were randomly divided into three groups of eight animals in each group. Control group of animals (0MR) was fed on a maize-based ration, whereas other two groups of animals (25MR & 50MR) were fed on rations where maize was replaced with tapioca root meal at 25 and 50 percentages respectively. The three rations i.e. 0MR, 25MR and 50MR were iso-nitrogenous and isocaloric. The average daily gain (ADG) in 50 per cent replacement group (50MR) increased significantly compared to control group (0MR). The digestibility of Dry Matter (DM), Organic Matter (OM), Ether Extract (EE) in 50 per cent replacement group (50MR) was higher compared to control group. Incidence of diarrhea was more in animals of the control group (0MR) and severity and duration of diarrhoea was reduced as the level of tapioca root meal inclusion increased in the diet. The result showed that, tapioca root meal can be included in the diet of cross bred pigs up to a level of 50 percent with distinct economic advantage.


Nahak A.K.,NRC on Mithun | Nahak A.K.,Orissa University of Agriculture and Technology | Giri S.C.,Regional Center | Giri S.C.,Orissa University of Agriculture and Technology | And 3 more authors.
Indian Journal of Animal Sciences | Year: 2014

This study was undertaken to investigate the relationship between methylene-blue reduction time (MBRT) and other semen quality parameters in duck by analysis of correlation coefficients. Semen ejaculates (32), collected at 2 days interval from 10 randomly selected drakes, were examined for different seminal parameters like pH, volume, colour, mass activity, individual motility, livability, concentration, number of spermatozoa per ejaculate, total sperm abnormality per cent and MBRT. The overall mean time to decolourise methylene blue in the white Pekin ducks was 10.03±0.24 min with a range from 8 to 13 min. MBRT was negatively correlated with mean spermatozoa concentration, number of spermatozoa per ejaculation and colour of semen samples but positively correlated with individual motility and livability. High correlation of MBRT with seminal parameters indicated its practical utility in monitoring semen characteristics.


Srivastava N.,Indian Veterinary Research Institute | Srivastava S.K.,Indian Veterinary Research Institute | Ghosh S.K.,Indian Veterinary Research Institute | Kumar A.,IVRI | And 2 more authors.
Asian Pacific Journal of Reproduction | Year: 2013

Objective: To evaluate the cryoinjury prediction of spermatozoa in relation to its cholesterol content at fresh and frozen-thaw stages. Methods: Ejaculates (n=12) were processed for cryopreservation, acrosome integrity (fluorescent and giemsa stains), cryoinjury (distribution of non capacitated, capacitated and acrosome reacted, pattern F, B and AR, respectively of Chlortetracycline, CTC assay), in vitro fertiltiy (IVF) and cholesterol content of spermatozoa at fresh, pre-freeze and frozen-thaw stages were evaluated. Values were fitted in prediction equation to predict acrosome integrity (AI) and cryoinjury. Results: Study indicated that cholesterol content of fresh spermatozoa can be used to predict cholesterol content of spermatozoa at pre-freeze and frozen-thaw stages of cryopreservation protocol with medium to high level of accuracy (P<0.05 and P<0.01, respectively). Cholesterol content of fresh spermatozoa can be used to predict AI, pattern B and AR and Penetration Index (PI) of IVF with medium level of accuracy (P<0.05) at frozen-thaw but not at pre-freeze stage. Similarly cholesterol content of frozen-thaw spermatozoa can be used to predict AI and pattern AR of frozen-thaw spermatozoa with medium level of accuracy (P<0.05). Conclusion: This study revealed strong evidence that cholesterol content of fresh as well as frozen-thaw bull spermatozoa can be a good predictor of level of cryoinjury following preservation at ultra low temperature. © 2013 Hainan Medical College.


Perumal P.,Animal Reproduction Laboratory | Srivastava S.K.,Indian Veterinary Research Institute | Ghosh S.K.,Indian Veterinary Research Institute | Baruah K.K.,NRC on Mithun | And 6 more authors.
Reproduction in Domestic Animals | Year: 2016

Artificial breeding of mithun poses several challenges including lack of standard protocol for cryopreservation of spermatozoa. This is further complicated by harmful effects of hen's egg yolk (EY) as additive in extender. Purified low-density lipoproteins (LDL) extracted from EY have been shown as beneficial over EY extender for long-term semen storage in several species. This investigation explored use of LDL versus EY on semen quality and oxidative stress following freezing–thawing of spermatozoa. A total of 25 of 50 ejaculates based on biophysical parameters were selected for the experiment. After diluting with the Tris-citrate-glycerol (TCG) extender, each sample was split into three equal aliquots: Group I, control, EY; Group II and Group III contained 8% and 10% purified LDL, respectively. Frozen–thawed samples were evaluated for motility parameters (progressive, and in the bovine cervical mucus penetration test [BCMPT]), viability, sperm and nuclear abnormality, acrosome integrity, and enzymatic (leakage of intracellular contents) and biochemical (oxidative stress) profiles and in vitro fertility (IVF) assay. Study revealed a significant (p <.05) improvement in viability, sperm and nuclear abnormality, acrosome integrity, motility (progressive and in cervical mucus), cholesterol content, and reduction in the leakage of intracellular enzymes in Group II. Moreover, intactness of acrosome and biochemical membranes was protected significantly (p <.05) in addition to significant (p <.05) improvement in binding per cent and binding index in IVF assay in extender containing 8% LDL. These results demonstrate that although cryopreservation of mithun's spermatozoa in EY was comparable with other species, addition of 8% LDL holds a clear advantage over EY or 10% LDL. © 2016 Blackwell Verlag GmbH


PubMed | Indian Veterinary Research Institute, ICAR Central Institute for Research on Cattle, ICAR NRC on Mithun, Animal Reproduction Laboratory and NRC on Mithun
Type: Journal Article | Journal: Reproduction in domestic animals = Zuchthygiene | Year: 2016

Artificial breeding of mithun poses several challenges including lack of standard protocol for cryopreservation of spermatozoa. This is further complicated by harmful effects of hens egg yolk (EY) as additive in extender. Purified low-density lipoproteins (LDL) extracted from EY have been shown as beneficial over EY extender for long-term semen storage in several species. This investigation explored use of LDL versus EY on semen quality and oxidative stress following freezing-thawing of spermatozoa. A total of 25 of 50 ejaculates based on biophysical parameters were selected for the experiment. After diluting with the Tris-citrate-glycerol (TCG) extender, each sample was split into three equal aliquots: Group I, control, EY; Group II and Group III contained 8% and 10% purified LDL, respectively. Frozen-thawed samples were evaluated for motility parameters (progressive, and in the bovine cervical mucus penetration test [BCMPT]), viability, sperm and nuclear abnormality, acrosome integrity, and enzymatic (leakage of intracellular contents) and biochemical (oxidative stress) profiles and in vitro fertility (IVF) assay. Study revealed a significant (p<.05) improvement in viability, sperm and nuclear abnormality, acrosome integrity, motility (progressive and in cervical mucus), cholesterol content, and reduction in the leakage of intracellular enzymes in Group II. Moreover, intactness of acrosome and biochemical membranes was protected significantly (p<.05) in addition to significant (p<.05) improvement in binding per cent and binding index in IVF assay in extender containing 8% LDL. These results demonstrate that although cryopreservation of mithuns spermatozoa in EY was comparable with other species, addition of 8% LDL holds a clear advantage over EY or 10% LDL.


Singh B.R.,ICAR Research Complex for NEH Region | Singh B.R.,Indian Veterinary Research Institute | Singh V.,NRC on Mithun | Singh R.K.,NRC on Mithun | And 3 more authors.
Medicinal Plants | Year: 2012

The study was conducted to determine antimicrobial activity of Artemisia vulgaris essential oil (AVEO), and to see the effect of drying of herb for AVEO extraction on its antimicrobial activity. AVEO was extracted from fresh chaffed herb and dried powdered herb and tested on 1199 strains of 113 species of pathogenic, potentially pathogenic and environmental microbes belonging to 33 different genera, 1172 were bacteria and 27 were yeast and moulds. Although more number of strains was sensitive for AVEO extracted from fresh herb (23%) than AVEO from dried herb (21%), difference was statistically insignificant (p, 0.40) between AVEOs extracted from fresh or dried herb. About 19.9% of bacterial and 25.9% of fungal isolates were sensitive to AVEO. Interestingly, oxidase positive strains (63.7%) including those of pseudomonads (60%), aeromonads (53.6%), spore forming bacilli (71.6%), Pastuerella (83.3%) and micrococci (66.7%) were comparatively more sensitive (p, <0.001) than oxidase negative bacteria (8.3%) to AVEO. Of the 114 clinical isolates (associated with illness in human and animals) belonging to 25 bacterial species, 23 (20.2%) were sensitive to AVEO. Clinical isolates were significantly (p, 0.03) more sensitive than isolates from healthy human and animals (12.6%). Thus for better antimicrobial activity AVEO should be extracted from fresh herb. The AVEO may be an effective therapeutic agent of future either as such or as the source of some novel antibacterial molecule(s) particularly against oxidase positive bacteria.

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