NRC on DNA Fingerprinting

Delhi, India

NRC on DNA Fingerprinting

Delhi, India
Time filter
Source Type

Dalamu,Indian Agricultural Research Institute | Behera T.K.,Indian Agricultural Research Institute | Gaikwad A.B.,NRC on DNA Fingerprinting | Saxena S.,NRC on DNA Fingerprinting | And 2 more authors.
Australian Journal of Crop Science | Year: 2012

Bitter gourd (Momordica charantia L.) is a nutritious vegetable crop of Asian origin. Fifty indigenous and exotic bitter gourd genotypes were characterized using 12 quantitatively inherited traits and genotypic variation was analysed using RAPD and ISSR markers. Based on the morphological traits, the first female flower appeared at lowest node (9th node) in gynoecious genotypes DBGy201 and DBGy202. Sel-2 possessed the highest yield per hectare (10,866 kg). Seventeen RAPD markers produced 84 amplicons in 50 accessions, of which 33 (41.34 %) were found polymorphic. Joint comparisons among the 50 accessions using Jaccard's similarity coefficient indicated that genetic distances (GD) ranged from 0.03 to 0.28. Eleven ISSR primers provided a total of 58 amplicons of which 41 (70.0 %) were polymorphic in 50 accessions. The Jaccard's similarity coefficient matrix values ranged from 0.50 to 0.95 based on molecular analysis. The polymorphic information content (PIC), resolving power (RP) and marker index (MI) were 0.17, 1.14 and 0.82, respectively for RAPD markers, whereas ISSR markers showed comparatively high polymorphic information content (0.40), resolving power (1.87), and marker index (2.11). The combined data analysis of RAPD and ISSR markers indicated that the relative polymorphism among accessions was 52.6 % with 2.64 polymorphic amplicons per primer. The value of average polymorphic information content, resolving power and marker index were 0.26, 1.42, and 1.33, respectively. These data demonstrate a large genetic variability among the Asian bitter gourd genotypes examined, which indicates that they should be considered as a valuable gene pool for bitter gourd breeding programs.

Singh S.,CCS Haryana Agricultural University | Mohapatra T.,Indian Agricultural Research Institute | Singh R.,NRC on DNA Fingerprinting | Hussain Z.,National Bureau of Plant Genetic Resources
Journal of Plant Biochemistry and Biotechnology | Year: 2013

An AFLP linkage map of Brassica juncea (L.) Czern and Coss was constructed using 88 recombinant inbred lines (RILs) from a cross between an Indian cultivar 'Varuna' and an accession from Poland 'BEC-144'. The map included 91 AFLP markers organized on 19 linkage groups covering a total map distance of 1679. 1 cM. A total of 14 QTLs were detected for oil content (2 QTLs), erucic acid (2 QTLs), eicosenoic acid (2 QTLs), linolenic acid (3 QTLs), linoleic acid (3 QTLs) and palmitic acid (2 QTLs). A specific genomic region on LG2 was associated with contents of three fatty acids: erucic acid, eicosenoic acid and linoleic acid. Some of the markers showed absolute linkage with the QTLs associated with the levels of linolenic acid, linoleic acid and oil content. These markers may be used for improvement of fatty acid profile of B. juncea. © 2012 Society for Plant Biochemistry and Biotechnology.

Verma M.,NRC on DNA Fingerprinting | Rathi S.A.D.,Indian Agricultural Research Institute | Munshi A.D.,Indian Agricultural Research Institute | Kumar M.A.,Indian Agricultural Research Institute | And 3 more authors.
Indian Journal of Horticulture | Year: 2012

Brinjal or eggplant (Solanum melongena L.) is one of the most important solanaceous vegetable crop plants after tomato and potato. Through the use of PCR based markers, genetic analysis in brinjal has gained momentum during the past few years. In this study, 11 RAPD and 6 SSR primers were used to analyze the genetic variation in 29 popular Indian brinjal varieties. The 11 RAPD primers generated 64 polymorphic markers with an average of 5.81 polymorphic bands per primer. Genetic distance based on RAPD markers among all the varieties ranged from 0.07 to 0.78 with an average of 0. 33. All the six SSR primer pairs were polymorphic with a total of 25 detected alleles. The number of alleles per primer ranged from 2 to 10, with a mean of 4. 67. UPGMA clustering for RAPD and SSR markers grouped all the brinjal varieties into two clusters, but grouping patterns were different for each of the marker system. Based on SSR markers, the maximum genetic distance of 1 was found between Pusa Bhairav and Green Long, Green Long and KS-224, Green Long and SL-195, Green Long and KS 331 and between Pusa Kranti and SL-195 followed by 0.85 between Pusa Kranti and KS-224, and NDB-25 and Pusa Kranti. However, majority of the cultivated varieties did not cluster concordant to the collection site information or phenotypic data such as fruit shape or any other known traits. The genetic diversity of brinjal varieties reported in this study will be useful when planning future crosses amongst these varieties.

Ramya P.,NRC on DNA Fingerprinting | Ramya P.,Indian Agricultural Research Institute | Bhat K.V.,NRC on DNA Fingerprinting | Pandey A.,NRC on DNA Fingerprinting | Pandey A.,ICAR Research Complex for NEH Region
Indian Journal of Agricultural Sciences | Year: 2012

The genetic variation available in wild and related species is a potential source for crop improvement. RIL population developed from a cross of Sesamum indicum and S. malabaricum consisting of 216 individuals along with the parental lines was evaluated for yield and other related traits. The traits considered were days to flowering, days to 50% flowering, days to capsule formation, leaf shape, hairiness, number of branches/plant, internode lengths (L1, L2, L3, L4 and L5), number of flowers/node, plant height, stem girth, days to maturity, capsule length, capsule width, number of capsules/ node, total number of capsules/plant, seed weight/capsule, yield/plant and 100- seed weight. Transgressive segregants falling far beyond parental values were noticed for many yield related traits. Correlation analysis indicated a strong positive correlation between yield and total number of capsules/plant, seed weight/capsule and 100- seed weight. Negative correlation was noticed in case of yield and average internode length L1. The results indicate the need to explore useful variability in the germplasm of the close wild progenitor species, S. malabaricum so as to overcome yield barriers in sesame due to domestication bottlenecks.

Kumar S.,NRC on DNA Fingerprinting | Kumar S.,University of Agriculture and Technology | Singh A.K.,NRC on DNA Fingerprinting | Jyoti,NRC on DNA Fingerprinting | Singh R.,NRC on DNA Fingerprinting
Vegetos | Year: 2011

Sixty genotypes were evaluated using morphological parameters and molecular markers. Considerable level of variability was observed among the genotypes for chlorophyll content, plant height, spike length and number of plant per meter. Maximum chlorophyll content was observed in genotype SACT-3.2-20-C under irrigated and EGPSN-28 under non-irrigated conditions, while, EGPSN-73 (irrigated) and SACT-2.5-9 (non-irrigated) showed highest density (plants/m 2). Seeds/ spike and 1000 grain weight were found significantly different in all the 60 genotypes. Genetic diversity based on morphophysiological traits grouped 60 genotypes in to three clusters and the cluster 3 was most distant group none of the genotype showed drought tolerance. Variability observed at morpho-physiological level was further confirmed by RAPD with 20 reproducible primers. RAPD analysis also grouped all 60 genotypes into three clusters except EGPSN-128 which was ungrouped. 64.4% mean similarity was observed among wheat genotypes, was an indication that genotypes have a common gene pool. Genotypes EGPSN73, SACT-3.3-17- A, EGPSN-58, HMN18 and EGPSN-144 were identified drought tolerant.

Singh A.K.,NRC on DNA Fingerprinting | Rana M.K.,NRC on DNA Fingerprinting | Singh S.,NRC on DNA Fingerprinting | Kumar S.,NRC on DNA Fingerprinting | And 2 more authors.
Journal of Plant Biochemistry and Biotechnology | Year: 2014

The availability of a simple, reproducible and cost-effective molecular marker is a prerequisite for plant genetic analysis. We have developed a novel promoter-targeted marker, CAAT box- derived polymorphism (CBDP) using the nucleotide sequence of CAAT box of plant promoters. CBDP, like random amplified polymorphic DNA (RAPD), uses single primer in polymerase chain reaction (PCR) for generating markers. However unlike RAPD, the CBDP primers are 18 nucleotides long and consist of a central CCAAT nucleotides core flanked by the filler sequence towards the 5′ end and di- or trinucleotides towards the 3′ end. In this study, a small set of 25 CBDP primer was designed and initially tested in a representative set of eight cultivars of jute for generation of polymorphic markers. Further, to achieve high reproducibility, a touchdown PCR was employed with an annealing temperature of 50oC. All the CBDP primers generated polymorphic markers in jute cultivars, and an UPGMA dendrogram based on Jaccard's similarity grouped them into two clusters represented by Corchorus capsularis and C. olitorius, respectively. Interestingly, such grouping of jute cultivars was consistent with genetic relationships established earlier for these cultivars using other DNA markers. Moreover, these CBDP primers also generated polymorphic markers in representative sets of cotton (Gossypium species) and linseed (Linum usitatissimum) cultivars. Given the high success rate of CBDP primers in generating markers in the tested species and advantages like ease in marker development and assay with reproducible profiles, they could potentially be exploited in other species as well for assessing genetic diversity, cultivar identification, construction of linkage map and marker- assisted selection. © 2013 Society for Plant Biochemistry and Biotechnology.

Ramya P.,NRC on DNA Fingerprinting | Bhat K.V.,NRC on DNA Fingerprinting
Indian Journal of Genetics and Plant Breeding | Year: 2012

India is an important centre of diversity for genus Abelmoschus belonging to family Malvaceae and about eight Abelmoschus species occur here. In the present study the phylogenetic relationships among A. caillei, A. crinitus, A. esculentus, A. ficulneus, A. moschatus, A. tuberculatus, A. tetraphyllus and A. pungens were analysed using modern phylogenetic tools such as nuclear ribosomal spacers (ITS-1, ITS-2) and chloroplast intergenic spacers (trnC-trnD, trnE-trnF, trnH-psbA)to estimate species divergence based on sequence similarity. The study exposed the lack of power of ribosomal spacers ITS-1and ITS-2 in resolving the species relationships in complex polyploid genera like Abelmoschus while the analysis of cpDNA intronic spacers revealed that A. ficulneus and A. moschatus are the closest wild relatives of A. esculentus. The study was helpful in redefining the Abelmoschus gene pool and since these species are exclusively found in the Indian sub continent, the centre of origin of okra should be re-addressed properly.

Loading NRC on DNA Fingerprinting collaborators
Loading NRC on DNA Fingerprinting collaborators