Cumberland, IN, United States
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Kern D.G.,Nu Skin International | Draelos Z.D.,Dermatology Consulting Services | Meadows C.,NOX Technologies Inc. | James Morre D.,NOX Technologies Inc. | Morre D.M.,NOX Technologies Inc.
Rejuvenation Research | Year: 2010

Activity of an age-related, superoxide-forming, cell-surface oxidase (arNOX) comparing dermis, epidermis, serum, and saliva from female and male subjects ages 28-72 years measured spectrophotometrically using reduction of ferricytochrome c correlated with oxidative skin damage as estimated from autofluoresence of skin using an Advanced Glycation End products Reader (AGE-Reader; DiagnOptics B.V., Netherlands). By reducing arNOX activity in skin with arNOX-inhibitory ingredients (NuSkin's ageLOC technology), skin appearance was improved through decreased protein cross-linking and an accelerated increase in collagen. © 2010 Mary Ann Liebert, Inc.


Morre D.J.,NOX Technologies Inc. | Morre D.M.,NOX Technologies Inc. | Shelton T.B.,NOX Technologies Inc.
Rejuvenation Research | Year: 2010

Aging-related cell-surface NADH oxidase (arNOX)-specific activities increase with age between age 30 and ages 50-65. The protein is shed and circulates. Activity correlates with a number of aging-related disorders including low-density lipoprotein (LDL) oxidation as a precondition to atherosclerosis as well as oxidation of collagen and elastin as a major contributor to skin aging. arNOX inhibitors formulated for sustained release are capable of maintaining circulating arNOX at low levels with regular use as food supplements formulated with natural compounds. Among the best sources are certain culinary seasonings, all of which are ingredients used extensively in the French kitchen. Their regular use may contribute to an understanding of the nutritional basis for the French Paradox. © 2010 Mary Ann Liebert, Inc.


Morre D.M.,NOX Technologies Inc. | Meadows C.,NOX Technologies Inc. | Morre D.J.,NOX Technologies Inc.
Rejuvenation Research | Year: 2010

An aging-related cell-surface oxidase (aging-related NADH oxidase, arNOX) generating superoxide and other reactive oxygen species is shed from the cell surface and is found in saliva, urine, perspiration, and interstitial fluids that surround the collagen and elastin matrix underlying dermis. arNOX activity correlates with age and reaches a maximum at about age 65 in males and 55 in females. arNOX activities are highly correlated with values of human skin where a causal relationship is indicated. Ongoing efforts focus on cloning arNOX proteins and development of antiaging formulas based on arNOX inhibition (intervention). © 2010 Mary Ann Liebert, Inc.


Wu L.-Y.,Purdue University | De Luca T.,Purdue University | Watanabe T.,NOX Technologies Inc. | Morre D.M.,Purdue University | And 3 more authors.
Biochimica et Biophysica Acta - General Subjects | Year: 2011

Background: Constituents and inhibitors of intermediary metabolism resulting in alterations in levels of cytosolic NADH, stimulation of sphingomyelinase and inhibition of sphingosine kinase were evaluated for effects on growth inhibition and induction of apoptosis by the ENOX2 inhibitors EGCG, the principal catechin of green tea, and phenoxodiol, a naturally occurring isoflavone. Methods: Responses were evaluated from dose-response curves of the metabolites and metabolic inhibitors in which growth of HeLa cells, apoptosis based on DAPI fluorescence and cytosolic NADH levels were correlated with sphingomyelinase and spingosine kinase activities and levels of ceramide and sphingosine1-phosphate. Results: Growth inhibition correlated with the modulation of localized cytosolic NADH levels by metabolites and metabolic inhibitors, the response of sphingomyelinase and sphingosine kinase located near the inner surface of the plasma membrane, and apoptosis. Conclusions: Based on findings with metabolites, we conclude that apoptosis in cancer cell lines caused by ENOX2 inhibitors such as EGCG and phenoxodiol is a direct response to elevated levels of cytosolic NADH that result from ENOX2 inhibition. General significance: The findings help to explain why increased NADH levels resulting from ENOX2 inhibition result in decreased prosurvival sphingosine-1-phosphate and increased proapoptotic ceramide, both of which may be important to initiation of the ENOX2 inhibitor-induced apoptotic cascade. © 2011 Elsevier B.V.


Trademark
Nox Technologies Inc. | Date: 2010-05-19

Non-medicated skin care preparations, namely, creams, lotions, gels, toners, cleansers, serums, facial masks, acne treatment, exfoliants for skin; cosmetics, namely, eye shadow, eye pencils, face powder, blush, lipstick, lip liner, liquid foundation, concealer. Dietary and nutritional supplements and meal replacement shakes. Skin treatment implements, namely, electrical applicators for applying cosmetics and lotions to the skin; kits comprising electrical applicators for applying cosmetics and lotions to the skin, and attachment brushes, sponges, and combs. Massage apparatus and instruments for massaging the skin.


PubMed | NOX Technologies Inc
Type: Journal Article | Journal: Rejuvenation research | Year: 2010

An aging-related cell-surface oxidase (aging-related NADH oxidase, arNOX) generating superoxide and other reactive oxygen species is shed from the cell surface and is found in saliva, urine, perspiration, and interstitial fluids that surround the collagen and elastin matrix underlying dermis. arNOX activity correlates with age and reaches a maximum at about age 65 in males and 55 in females. arNOX activities are highly correlated with values of human skin where a causal relationship is indicated. Ongoing efforts focus on cloning arNOX proteins and development of antiaging formulas based on arNOX inhibition (intervention).


Described are cell surface and circulating markers for aging related disorders (specific isoforms of NADH oxidase (arNOX)). Recombinant age-related NADH oxidase isoforms and their coding sequences and methods for detecting arNOX isoform presence and quantitation in tissues and in blood, sera, urine, saliva, perspiration and in other body fluids, are provided. Recombinant arNOX proteins are useful in preparing antigens for use in the generation of monoclonal and polyclonal antibodies as well as immunogenic compositions for diagnosis and treatment of aging disorders. DNA probes based on the DNA sequence information provide may be used to identify individuals at risk for aging disorders and for development of therapeutic interventions or anti-aging cosmetic or other formulations of benefit in slowing the aging process in mammals.


Embodiments herein relate to agents that mimic calorie restriction (CR) to extend life span, especially agents that lower the level of cytosolic NADH and increase the level of cytosolic NAD+ (relative to NADH) required to mimic calorie restriction that results in life span extension, at least in part by inhibition by NADH of SIR2, which is a key regulator of life span in both yeast and animals. NADH is a competitive inhibitor of SIR2, an NAD-requiring NAD-dependent histone deacetylase required for chromatin silencing and life-span extension. An overall reduction in NADH activates SIR2 and extends life span. The use of fluorescence measurements of NADH levels in a 96-well plate assay utilizing Bakers yeast (Saccharomyces cerevisiae) as a model to screen for agents and supplements that lower NADH levels is disclosed herein. A caloric restriction mimetic contains 1 part Mg2+ malate, 1 part turmeric and 98 parts dehydrated potato skins.


Trademark
Nox Technologies Inc. | Date: 2014-04-02

Non-medicated skin care preparations, namely, creams, lotions, gels, toners, cleansers, serums, facial masks, acne treatment, exfoliants for skin; cosmetics, namely, eye shadow, eye pencils, face powder, blush, lipstick, lip liner, liquid foundation, concealer; Dietary and nutritional supplements and meal replacement shakes; Skin treatment implements, namely, electrical applicators for applying cosmetics and lotions to the skin; kits comprising electrical applicators for applying cosmetics and lotions to the skin, and attachment brushes, sponges, and combs; and Massage apparatus and instruments for massaging the skin.


PubMed | NOX Technologies Inc
Type: Comparative Study | Journal: Rejuvenation research | Year: 2010

Aging-related cell-surface NADH oxidase (arNOX)-specific activities increase with age between age 30 and ages 50-65. The protein is shed and circulates. Activity correlates with a number of aging-related disorders including low-density lipoprotein (LDL) oxidation as a precondition to atherosclerosis as well as oxidation of collagen and elastin as a major contributor to skin aging. arNOX inhibitors formulated for sustained release are capable of maintaining circulating arNOX at low levels with regular use as food supplements formulated with natural compounds. Among the best sources are certain culinary seasonings, all of which are ingredients used extensively in the French kitchen. Their regular use may contribute to an understanding of the nutritional basis for the French Paradox.

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