Novosibirsk State Agricultural University
Novosibirsk, Russia
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Shekhovtsov S.V.,RAS Institute of Cytology and Genetics | Bazarova N.E.,Novosibirsk State Agricultural University | Berman D.I.,Russian Academy of Sciences | Bulakhova N.A.,Russian Academy of Sciences | And 5 more authors.
Russian Journal of Genetics: Applied Research | Year: 2017

Earthworms are a widespread and ecologically important group of animals, which has the highest total biomass in some ecosystems and often defines the composition of soil fauna. Earthworms are known to have high cryptic genetic diversity. In this study we attempted to estimate earthworm species diversity in the south of West Siberia by DNA barcoding. This method employs short fragments of the genome to identify species, and allows one to work with specimens that cannot be identified by conventional techniques, as well as to search for new species and predict their phylogenetic affinities. As the target sequence we took a fragment of the mitochondrial cytochrome oxidase 1 (cox1) gene. The studied territory (Novosibirsk and Tomsk oblasts, Altai krai, and the Altai Republic) is known to contain 16 species and subspecies of earthworms. We analyzed 259 individuals from twelve locations and detected 27 genetic clusters. Ten of them correspond to known species (A. caliginosa, E. fetida, O. tyrtaeum, D. rubidus tenuis, D. octaedra, E. balatonica, E. sibirica, as well as three genetic lineages ofE. nordenskioldi nordenskioldi). Seventeen of the 27 clusters do not have close sequence similarity to any known earthworm species. Representatives of some of these novel clusters are morphologically similar to the Eisenia n. nordenskioldi/E. n. pallida species complex and may belong to new genetic lineages of this complex. The rest of the novel clusters probably represent new earthworm species. Therefore, we can conclude that a large portion of earthworm biodiversity in the south of West Siberia is still unexplored. © 2017, Pleiades Publishing, Ltd.

Trapezov O.V.,RAS Institute of Cytology and Genetics | Zemljanitskaja E.I.,Novosibirsk State Agricultural University | Rasputina O.V.,Novosibirsk State Agricultural University | Naumkin I.V.,Novosibirsk State Agricultural University | Trapezova L.I.,RAS Institute of Cytology and Genetics
Russian Journal of Genetics | Year: 2016

The different role of coat color mutations in the American mink on the per os effect of the biologically active preparation Biostyl was shown. The number of kits per female was the same in all control genotypes, including Standard (+/+ +/+), sapphire (a/a p/p), and lavender (a/a m/m): 4.4 ± 0.4, 4.4 ± 0.5, and 4.3 ± 0.5, respectively. Experimental groups of these genotypes have shown a great contrast among each other: stimulation of the reproductive function was 5.2 ± 0.3 in Standard minks, while suppression of the reproductive function was 3.8 ± 0.6, and 2.3 ± 0.5 in the double recessive mutants sapphire and lavender, respectively. The differentiation in body mass between experimental and control newborn Standard kits was not revealed. A significant decrease in the body mass of newborn experimental sapphire kits as compared to control group in a sex-specific manner was registered. © 2016, Pleiades Publishing, Inc.

Amstislavsky S.Ya.,Novosibirsk State University | Kizilova E.A.,Novosibirsk State University | Brusentsev E.Yu.,RAS Institute of Cytology and Genetics | Abramova T.O.,RAS Institute of Cytology and Genetics | Naprimerov V.A.,Novosibirsk State Agricultural University
Zoologicheskii Zhurnal | Year: 2016

The aim of the present study was an attempt of cryopreservation of Djungarian (P. sungorus) and Campbell's (P. campbelli) hamsters' semen. Epididymal semen of these two Phodopus species was frozen using three dif ferent cryoprotectant agents (CPA): raffinose and skimmed milk; CaniPlus Chill supplemented with egg yolk and glycerol; and CaniPlus Freeze. The viability of frozen-thawed and non-frozen spermatozoa was evaluated using double staining with SYBR Green I+PI and subsequent confocal microscopy; the sperm morphology was assessed by light microscopy. This study is the first report of the successful semen cryopreservation in Phodopus species. The results of the present study permit to conclude that the methods of semen freezing/thawing/cryopreservation usingf CaniPlus Chill supplemented with egg yolk glycerol and/or CaniPlus Freeze are applicable for the cryopreservation of spermatozoa of Djungarian and Campbell's hamsters and may be considered as promising cryoprotectants for semen freezing in other mammalian species.

PubMed | Leibniz Center for Agricultural Landscape Research and Novosibirsk State Agricultural University
Type: Journal Article | Journal: Journal of applied microbiology | Year: 2016

To identify the taxonomic differences between phytopathogenic small-spored Alternaria strains isolated from wheat kernels in Germany and Russia by a polyphasic approach.Ninety-five Alternaria (A.) strains were characterized by their colony colour, their three-dimensional sporulation patterns, mycotoxin production and phylogenetic relationships based on sequence variation in translation elongation factor 1- (TEF1-). The examination of toxin profiles and the phylogenetic features via TEF1- resulted in two distinct clusters, in each case containing Alternaria infectoria isolates (92 and 96% respectively) in the first and the Alternaria alternata, Alternaria arborescens and Alternaria tenuissima isolates (77 and 79% respectively) in the other combined cluster. The production of Alternariol, Altertoxin and Altenuene has not been reported previously in the A.infectoria species group. The isolates from Germany and Russia differ slightly in species composition and mycotoxin production capacity.We identified that the A.infectoria species group can be differentiated from the A.alternata, A.arborescens and A.tenuissima species group by colour, low mycotoxin production and by the sequence variation in TEF1- gene.These results allow a reliable toxic risk assessment when detecting different Alternaria fungi on cereals.

Isachenko E.,Novosibirsk State University | Orlik E.,Novosibirsk State Agricultural University | Kabov O.,Tomsk Polytechnic University
MATEC Web of Conferences | Year: 2016

The experimental setup has been designed and fabricated to study the evaporation of liquid droplets, blown by the gas flow in a mini-channel with a height of 6 and 9 mm. Substrates were removable, and the surface temperature of the substrate was maintained at a constant level. The shadow method was the main method of measurement. A series of experiments with a water drop of 100 μl volume settled on a polished stainless substrate were carried out. As a result the dependences for the droplet evaporation rate were obtained in the range of temperatures 25-70 °C and Reynolds numbers of gas 0-2000. © The Authors, published by EDP Sciences, 2016.

Biltueva L.,Novosibirsk State University | Kulemzina A.,Novosibirsk State University | Vorobieva N.,Novosibirsk State University | Perelman P.,Novosibirsk State University | And 3 more authors.
Cytogenetic and Genome Research | Year: 2014

This study reports on a unique balanced reciprocal chromosome translocation detected in a phenotypically normal cattle dam and her calf. CBG-, GTG-banding and FISH using bovine whole-chromosome and telomere probes were applied. The analyses showed that the breakpoints were located near to the centromere in chromosome 26 (q11) and exceptionally close to the telomere in chromosome 13 (q24). The whole euchromatin segment of chromosome 26 was translocated onto chromosome 13. The distal end of chromosome 13 was translocated to the subcentromeric region of chromosome 26. We describe this aberration as a balanced reciprocal translocation rcp(13;26)(q24;q11). It appears that this aberration was maternally derived and may have originated de novo in the dam. © 2014 S. Karger AG, Basel.

Syso A.I.,Novosibirsk State Agricultural University | Syromlya T.I.,Russian Academy of Sciences | Myadelets M.A.,Novosibirsk State Agricultural University | Cherevko A.S.,Russian Academy of Sciences
Contemporary Problems of Ecology | Year: 2016

The content of 30 macro- and microelements has been studied and assessed by statistical, hygienic, and biochemical criteria in soils and Achillea millefolium L. as a forage and medicinal plant. The study has been conducted in the territories of the Novosibirsk agglomeration contaminated by waste from industry and transport. The relationships between elemental and biochemical compositions of Ach. millefolium and in the soil–plant system are determined. © 2016, Pleiades Publishing, Ltd.

Valentov A.V.,Tomsk Polytechnic University | Konovodov V.V.,Novosibirsk State Agricultural University | Agafonova E.V.,Novosibirsk State Agricultural University
Applied Mechanics and Materials | Year: 2013

The results indicate a reduction of defects in soldering turning tools through optimal dimensions of a solder. In this paper stress in a flat insert is calculated and the results of laboratory tests of cutters iron-carbon soldered are presented. The optimal dimensions of the solder are selected according to calculations conducted. © (2013) Trans Tech Publications, Switzerland.

Konovodov V.V.,Novosibirsk State Agricultural University | Valentov A.V.,Tomsk Polytechnic University | Kukhar I.S.,Tomsk Polytechnic University
IOP Conference Series: Materials Science and Engineering | Year: 2015

The study is focused on the analysis of the influence of short-term exposure to high temperature on tungstenless hard alloy metal plates while soldering iron-carbon. According to the results of the research with macrohardness tester the macrohardness deviation amounted to 3%. © Published under licence by IOP Publishing Ltd.

Bokova T.I.,Novosibirsk State Agricultural University | Vasil'tsova I.V.,Novosibirsk State Agricultural University
Contemporary Problems of Ecology | Year: 2011

Lead and cadmium migration was studied in the system: biologically active plant raw material-extract. Antioxidant activity and detoxication ability of the extracts of birch buds and leaves was studied in pattern experiments with rats. © 2011 Pleiades Publishing, Ltd.

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