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Konovodov V.V.,Novosibirsk State Agricultural University | Valentov A.V.,Tomsk Polytechnic University | Kukhar I.S.,Tomsk Polytechnic University
IOP Conference Series: Materials Science and Engineering | Year: 2015

The study is focused on the analysis of the influence of short-term exposure to high temperature on tungstenless hard alloy metal plates while soldering iron-carbon. According to the results of the research with macrohardness tester the macrohardness deviation amounted to 3%. © Published under licence by IOP Publishing Ltd. Source

Kahl S.M.,Leibniz Center for Agricultural Landscape Research | Kahl S.M.,University of Potsdam | Ulrich A.,Leibniz Center for Agricultural Landscape Research | Kirichenko A.A.,Novosibirsk State Agricultural University | And 2 more authors.
Journal of Applied Microbiology | Year: 2015

Aims: To identify the taxonomic differences between phytopathogenic small-spored Alternaria strains isolated from wheat kernels in Germany and Russia by a polyphasic approach. Methods and Results: Ninety-five Alternaria (A.) strains were characterized by their colony colour, their three-dimensional sporulation patterns, mycotoxin production and phylogenetic relationships based on sequence variation in translation elongation factor 1-α (TEF1-α). The examination of toxin profiles and the phylogenetic features via TEF1-α resulted in two distinct clusters, in each case containing Alternaria infectoria isolates (92 and 96% respectively) in the first and the Alternaria alternata, Alternaria arborescens and Alternaria tenuissima isolates (77 and 79% respectively) in the other combined cluster. The production of Alternariol, Altertoxin and Altenuene has not been reported previously in the A. infectoria species group. The isolates from Germany and Russia differ slightly in species composition and mycotoxin production capacity. Conclusions: We identified that the A. infectoria species group can be differentiated from the A. alternata, A. arborescens and A. tenuissima species group by colour, low mycotoxin production and by the sequence variation in TEF1-α gene. Significance and Impact of the Study: These results allow a reliable toxic risk assessment when detecting different Alternaria fungi on cereals. © 2015 The Society for Applied Microbiology. Source

Amstislavsky S.Ya.,Novosibirsk State University | Kizilova E.A.,Novosibirsk State University | Brusentsev E.Yu.,RAS Institute of Cytology and Genetics | Abramova T.O.,RAS Institute of Cytology and Genetics | Naprimerov V.A.,Novosibirsk State Agricultural University
Zoologicheskii Zhurnal | Year: 2016

The aim of the present study was an attempt of cryopreservation of Djungarian (P. sungorus) and Campbell's (P. campbelli) hamsters' semen. Epididymal semen of these two Phodopus species was frozen using three dif ferent cryoprotectant agents (CPA): raffinose and skimmed milk; CaniPlus Chill supplemented with egg yolk and glycerol; and CaniPlus Freeze. The viability of frozen-thawed and non-frozen spermatozoa was evaluated using double staining with SYBR Green I+PI and subsequent confocal microscopy; the sperm morphology was assessed by light microscopy. This study is the first report of the successful semen cryopreservation in Phodopus species. The results of the present study permit to conclude that the methods of semen freezing/thawing/cryopreservation usingf CaniPlus Chill supplemented with egg yolk glycerol and/or CaniPlus Freeze are applicable for the cryopreservation of spermatozoa of Djungarian and Campbell's hamsters and may be considered as promising cryoprotectants for semen freezing in other mammalian species. Source

Novosibirsk State Agricultural University | Entity website

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