Cambridge, MA, United States
Cambridge, MA, United States

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Shaw A.J.,Mascoma Corporation | Shaw A.J.,Novogy Inc. | Guss A.,Oak Ridge National Laboratory | Bahl H.,University of Rostock | Lynd L.R.,Mascoma Corporation
Metabolic Engineering | Year: 2014

The thermophilic anaerobe Thermoanaerobacterium saccharolyticum JW/SL-YS485 was investigated as a host for n-butanol production. A systematic approach was taken to demonstrate functionality of heterologous components of the clostridial n-butanol pathway via gene expression and enzymatic activity assays in this organism. Subsequently. , integration of the entire pathway in the wild-type strain resulted in n-butanol production of 0.85. g/L from 10. g/L xylose, corresponding to 21% of the theoretical maximum yield. We were unable to integrate the n-butanol pathway in strains lacking the ability to produce acetate, despite the theoretical overall redox neutrality of n-butanol formation. However, integration of the n-butanol pathway in lactate deficient strains resulted in n-butanol production of 1.05. g/L from 10. g/L xylose, corresponding to 26% of the theoretical maximum. © 2013.


Deng Y.,Dartmouth College | Deng Y.,BioEnergy Science Center | Olson D.G.,Dartmouth College | Olson D.G.,BioEnergy Science Center | And 9 more authors.
Metabolic Engineering | Year: 2013

In Clostridium thermocellum, a thermophilic anaerobic bacterium able to rapidly ferment cellulose to ethanol, pyruvate kinase (EC 2.7.1.40) is absent based on both the genome sequence and enzymatic assays. Instead, a new pathway converting phosphoenolpyruvate to pyruvate via a three-step pathway involving phosphoenolpyruvate carboxykinase, NADH-linked malate dehydrogenase, and NADP-dependent malic enzyme has been found. We examined the impact of targeted modification of enzymes associated with this pathway, termed the "malate shunt", including expression of the pyruvate kinase gene from Thermoanaerobacterium saccharolyticum, mutation of the phosphoenolpyruvate carboxykinase and deletion of malic enzyme gene. Strain YD01 with exogenous pyruvate kinase, in which phosphoenolpyruvate carboxykinase expression was diminished by modifying the start codon from ATG to GTG, exhibited 3.25-fold higher ethanol yield than the wild-type strain. A second strain, YD02 with exogenous pyruvate kinase, in which the gene for malic enzyme and part of malate dehydrogenase were deleted, had over 3-fold higher ethanol yield than the wild-type strain. © 2012 Elsevier Inc.


Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.


Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical phosphorus or sulfur sources. Fermentation methods using the genetically engineered organisms are also described. The fermentation methods are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals.


DGA1 catalyzes the final enzymatic step for converting acyl-CoA and 1,2-diacylglycerol to triacylglycerols (TAG) and CoA in yeast. Disclosed are methods for expression in an oleaginous yeast host of polynucleotide sequences encoding DGA1 from Rhodosporidium toruloides, Lipomyces starkeyi, Aurantiochytrium limacinium, Aspergillus terreus, or Claviceps purpurea. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising heterologous DGA1 polynucleotides encoding DGA1 proteins, or functionally active portions thereof, having the capability of producing increased lipid production and possessing the characteristic of enhanced glucose consumption efficiency.


Trademark
Novogy Inc. | Date: 2013-11-26

Fermentation media chemicals for use in the production of cells and biologically produced compounds; biochemicals, namely, oleochemicals and fatty acids for use as raw ingredients in the production of fuels, food, and animal feed. Animal feed supplements; animal feed additive for use as a nutritional supplement for medical purposes; non-medicated additives for animal feed for use as nutritional supplements; animal feed additives, namely, proteins, fats, carbohydrates, amino acids, vitamins and minerals; food supplements; vitamin and mineral supplements for use as ingredients in the food industry; nutritional supplements; protein supplements. Animal feed.


Trademark
Novogy Inc. | Date: 2012-10-23

Biofuels and renewable fuels. Energy management services, namely, providing a service that allows customers to purchase energy, namely, electricity, natural gas and renewable energy, at a fixed price. Production, treatment and refinement of fuel, diesel fuel, biofuel and biodiesel fuel for others. Licensing of intellectual property.


Trademark
Novogy Inc. | Date: 2016-06-03

Biofuels and renewable fuels; industrial lubricants. Energy management services, namely, providing a service that allows customers to purchase energy, namely, electricity, natural gas and renewable energy, at a fixed price. Production, treatment and refinement of fuel, diesel fuel, biofuel and biodiesel fuel for others. Licensing of intellectual property.


Trademark
Novogy Inc. | Date: 2013-07-17

Microorganisms for use in the fermentation of biofuels and biochemicals; fermentation media chemicals for use in the production of cells and biologically produced compounds.


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