Chongqing Shi, China
Chongqing Shi, China
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Gerencser A.A.,Buck Institute for Research on Aging | Gerencser A.A.,Touro College | Gerencser A.A.,Nova Software | Mulder H.,Lund University | And 2 more authors.
Biochemical Journal | Year: 2015

In the presence of high glucose or pyruvate, INS-1 832/13 insulinoma cells undergo stochastic oscillations in plasma membrane potential (Δψp) leading to associated fluctuations in cytosolic free Ca2+ concentration ([Ca2+]c). Oscillations are not driven by upstream metabolic fluctuations, but rather by autonomous ionic mechanisms, the details of which are unclear. We have investigated the nature of the oscillator, with simultaneous fluorescence monitoring of Δψp, [Ca2+]c and exocytosis at single-cell resolution, combined with analysis of the occurrence, frequency and amplitude of Δψp oscillations. Oscillations were closely coupled to exocytosis, indicated by coincident synaptopHluorin fluorescence enhancement. L-type Ca2+ channel inhibitors enhanced Δψp and [Ca2+]c oscillation frequency in the presence of pyruvate, but abolished the sustained [Ca2+]c response following KCl depolarization. The L-type Ca2+ channel inhibitor isradipine did not inhibit oscillation-linked exocytosis. The T-type Ca2+ channel inhibitor NNC 55-0396 inhibited Δψp and [Ca2+]c oscillations, implying that T-type Ca2+ channels trigger oscillations and consequent exocytosis. Since distinct ion channels operate in oscillating and non-oscillating cells, quantitative analysis of Δψp and [Ca2+]c oscillations in a β-cell population may help to improve our understanding of the link between metabolism and insulin secretion. © 2015 Authors.


da Costa Mattos H.S.,Federal University of Fluminense | Dias R.A.C.,Nova Software
International Communications in Heat and Mass Transfer | Year: 2013

The present paper is concerned with the modeling of hyperbolic heat transfer in generalized Newtonian fluids. A general procedure, developed within the framework of thermodynamics of irreversible processes, is proposed to obtain constitutive relations that verify automatically the second law of thermodynamics and the principle of material objectivity. Such a thermodynamic approach allows a rational identification of the terms responsible for the thermomechanical coupling in the heat equation, which is a first step to better understand its influence on the fluid behavior. © 2012 .


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Nova News: German Partner Floyd Software Outsourcing Visits Nova Software Headquarters in Chongqing Last week, Mr. Douglas Floyd (from the Berlin area of Germany) visited Nova Software to discuss how we cooperate with each other in software outsourcing business in Germany ...


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A US-based company decided to use Nova's technical expertise to redesign a series of web sites, including designing the appearance of the whole web site, maintaining the website content, and developing some new modules with special features. Since all the web sites shared similar basic functionalities, we used DotNetNuke, an open source framework, to develop all the web sites for the purpose of reduceing development cost ...


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