Reddy S.,Norwich Clinical Services Pvt. Ltd. |
Pattabi A.,Norwich Clinical Services Pvt. Ltd. |
Kumar A.,Norwich Clinical Services Pvt. Ltd. |
Rajpurohit S.,Norwich Clinical Services Pvt. Ltd. |
And 2 more authors.
Research Journal of Pharmacy and Technology | Year: 2013
Gliclazide and Glipizide (IS) were separated on a Reverse phase chromatography using the mobile phase mixture of ammonium acetate and methanol at a flow rate of 1.0 ml/min after extraction from plasma by liquid - liquid extraction method. Both the analyte and the IS were detected in API 4000 Mass spectrometer in the positive atmospheric pressure Ionization (API) mode with multiple reactions monitoring (MRM). The MRM transitions were monitored by following m/z for parent ion 324.1 and daughter ion 127.2 (Gliclazide), and m/z 446.1 and daughter 321.1 (Glipizide, IS). A linear calibration plot of Gliclazide was achieved in the concentration ranges of 7.776 ng/ml to 6000 ng/ml. Mean recovery was 75.67%.This method was fully validated for specificity, precision, accuracy, reproducibility and other criteria as per regulations. © RJPT.
Reddy S.,Norwich Clinical Services Pvt Ltd |
Ahmed I.,Norwich Clinical Services Pvt Ltd |
Ahmad I.,Norwich Clinical Services Pvt Ltd |
Mukhopadhyay A.,Norwich Clinical Services Pvt Ltd |
Thangam S.,Norwich Clinical Services Pvt Ltd
Journal of Chromatographic Science | Year: 2015
A simple, sensitive, precise and accurate method for simultaneous estimation of metformin and sitagliptin from human plasma was developed and validated. Samples extracted from plasma using acetonitrile were separated on an SCX column and estimated using API 4000 Mass Spectrometer in the positive atmospheric pressure ionization mode (Turboionspray) by following multiple reaction monitoring transitions for both parent and daughter ions. A linear calibration plot was achieved for both the analytes in the concentration ranges of 10-2,206 ng/mL (for metformin) and 3-800.5 ng/mL (for sitagliptin) prepared in K2EDTA pooled plasma. Mean recovery for metformin was 92% and for sitagliptin was 104.5%. It is a fully validated method and successfully applied for estimation of these drug molecules during biostudies. © The Author 2015. Published by Oxford University Press.