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Miyata S.,Japan National Agriculture and Food Research Organization | Kato H.,Japan National Agriculture and Food Research Organization | Davis R.,Northern Australia Quarantine Strategy | Smith M.W.,Bundaberg Research Station | And 2 more authors.
Journal of General Plant Pathology | Year: 2011

'Candidatus Liberibacter asiaticus' is the most widespread of the three species of 'Ca. Liberibacter' that cause citrus greening disease (huanglongbing). To ascertain the phylogenetic relationships among Indian isolates that have higher diversity in the 16S rDNA than Asian isolates of this species, we collected symptomatic leaves from Northeast India, Papua New Guinea and Timor-Leste (East Timor) and detected 'Ca. L. asiaticus' by PCR using primers specific for nusG-rplK genes and 16S rDNA. Phylogenetic analysis with 16S rDNA sequences and single nucleotide polymorphisms of the omp gene region revealed that the Northeast Indian isolates were genetically closer to Asian-common isolates from Japan, Taiwan, and Vietnam than to Indian isolates reported previously. Thus, the Asian-common strains of 'Ca. L. asiaticus' are apparently also present in Northeast India. © 2010 The Phytopathological Society of Japan and Springer.

Lu B.,Key Laboratory of Integrated Pest Management on Tropical crops | Lu B.,Chinese Academy of Sciences | Zhu W.,Key Laboratory of Integrated Pest Management on Tropical crops | Zhu W.,Chinese Academy of Sciences | And 9 more authors.
Biocontrol Science and Technology | Year: 2014

To optimise the production of Asecodes hispinarum Bouček (Hymenoptera: Eulophidae), a parasitoid of coconut leaf beetle, Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae), some of the factors affecting rates of parasitism, number of offspring produced per host and sex ratio of A. hispinarum were investigated. The numbers and sex ratio of A. hispinarum offspring per host reduced significantly at extreme low humidity (30% relative humidity [RH]), but there was no significant effect on parasitism. Photoperiod had no significant effects on any of the life traits tested. A. hispinarum was able to reproduce via arrhenotoky, and while increasing the proportion of female parents increased the number of parasitoids produced, the proportion of female offspring decreased. Older females showed a lower rate of parasitism than young females, however, maternal age did not affect the number or the sex ratio of offspring. Increasing the number of hosts offered to a pair of parasitoids significantly increased the number of parasitised hosts but decreased the parasitism rate while the sex ratio of progeny was not affected. Present work showed that to maximise the production of female parasitoids, a parasitoid/host ratio of 1:1, using one-day old A. hispinarum at a female/male ratio of 3:1 and RH of at least 55% is recommended. © 2013 © 2013 Taylor & Francis.

Katoh H.,Japan National Agriculture and Food Research Organization | Davis R.,Northern Australia Quarantine Strategy | Smith M.W.,Bundaberg Research Station | Weinert M.,Center for Tropical Agriculture | Iwanami T.,Japan National Agriculture and Food Research Organization
Annals of Applied Biology | Year: 2012

Japanese isolates of 'Candidatus Liberibacter asiaticus' have been shown to be clearly differentiated by simple sequence repeat (SSR) profiles at four loci. In this study, 25 SSR loci, including these four loci, were selected from the whole-genome sequence and were used to differentiate non-Japanese samples of 'Ca. Liberibacter asiaticus' (13 Indian, 3 East Timorese, 1 Papuan and 8 Floridian samples). Out of the 25 SSR loci, 13 were polymorphic. Dendrogram analysis using SSR loci showed that the clusters were mostly consistent with the geographical origins of the isolates. When single nucleotide polymorphisms (SNPs) were searched around these 25 loci, only the upstream region of locus 091 exhibited polymorphism. Phylogenetic tree analysis of the SNPs in the upstream region of locus 091 showed that Floridian samples were clustered into one group as shown by dendrogram analysis using SSR loci. The differences in nucleotide sequences were not associated with differences in the citrus hosts (lime, mandarin, lemon and sour orange) from which the isolates were originally derived. © 2012 Association of Applied Biologists.

Bellis G.A.,Northern Australia Quarantine Strategy | Donaldson J.F.,83 Mills Road | Quintao V.,Directorate of Quarantine and Biosecurity | Rice A.,Northern Australia Quarantine Strategy | And 2 more authors.
Austral Entomology | Year: 2014

Examination of Delphacini holdings in Australian insect collections and comparison with material from overseas collections has revealed several species not previously recorded from Australia, Timor Leste and/or Papua New Guinea. Newly recorded species from Australia are Anchodelphax olenus Fennah, Cemus sauteri (Muir), Falcotoya auriniaFennah, Hagamiodes fuscicaudata (Muir), Horcoma colorata lacteipennis (Muir), Latistria placitus (van Duzee), Nemetor sabinusFennah, Nilaparvata bakeri (Muir), Nilaparvata myersi (Muir), Numata corporaali (Muir), Nycheuma coctum (Yang), Perkinsiella bakeri (Muir), Rhombotoya pseudonigripennis (Muir), Tagosodes pusanus (Distant), Toya bridwelli (Muir). Newly recorded species from Timor Leste are Falcotoya aurinia, Horcoma colorata lacteipennis, Latistria placitus, Nycheuma coctum and Tagosodes pusanus. Newly recorded species from Papua New Guinea are Hagamiodes fuscicaudata and Laodelphax striatellus (Fallén). An updated checklist of Australian Delphacini is provided. [Correction added on 19 December 2013, after first online publication: 'Laodelphax striatellus' has been removed from the list of newly recorded species.] © 2013 Australian Entomological Society.

Bellis G.A.,Northern Australia Quarantine Strategy | Gopurenko D.,Australian Department of Primary Industries and Fisheries | Gopurenko D.,Graham Center for Agricultural Innovation | Cookson B.,AAC International | And 5 more authors.
Austral Entomology | Year: 2015

Light trap surveillance across northern Australia and Papua New Guinea (PNG) has detected the presence of several Oriental species of Culicoides not previously reported from those countries and which appear to have arrived in recent times. Detections of C.nudipalpisDelfinado in Western Australia, C.flavipunctatusKitaoka and C.palpiferDas Gupta and Ghosh in the Northern Territory and of C.flavipunctatus, C.fulvusSen and Das Gupta and C.orientalisMacfie in Queensland (Qld) provide evidence of multiple pathways for incursions of biting midges into northern Australia. Of these, only C.fulvus appears to have established. Additionally, three species, C.fulvus, C.wadai Kitaoka and C.brevipalpisDelfinado, are newly reported from PNG and all appear to be well established. The arrival in PNG of C.fulvus and C.brevipalpis, both not previously reported from Qld, suggests that pathways exist for the entry of Oriental insects into New Guinea directly from Asia, rather than via Australia. Molecular analyses using DNA barcodes (partial mitochondrial cytochrome oxidase subunit one sequences) confirmed morphological identification of specimens and additionally provided strong evidence relating to the source of these incursions. At least two of these species are vectors of important livestock viruses and are likely to impact on the epidemiology of these viruses as they continue to disperse. © 2015 Australian Entomological Society.

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