North East Agricultural University

Harbin, China

North East Agricultural University

Harbin, China

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Li X.,Harbin Medical University | Li X.,Qiqihar Medical University | Shan Z.-Y.,Harbin Medical University | Wu Y.-S.,Harbin Medical University | And 5 more authors.
Reproduction | Year: 2014

Pig pluripotent cells may represent an advantageous experimental tool for developing therapeutic application in the human biomedical field. However, it has previously been proven to be difficult to establish from the early embryo and its pluripotency has not been distinctly documented. In recent years, induced pluripotent stem (iPS) cell technology provides a new method of reprogramming somatic cells to pluripotent state. The generation of iPS cells together with or without certain small molecules has become a routine technique. However, the generation of iPS cells from pig embryonic tissues using viral infections together with small molecules has not been reported. Here, we reported the generation of induced pig pluripotent cells (iPPCs) using the iPS technology in combination with valproic acid (VPA). VPA treatment significantly increased the expression of pluripotent genes and played an important role in early reprogramming. We showed that iPPCs resembled pig epiblast cells in their morphology and pluripotent markers, such as OCT4, NANOG, and SSEA1. It had a normal karyotype and could form embryoid bodies, which express three germ layer markers in vitro. In addition, the iPPCs might directly differentiate into neural progenitors after being induced with the retinoic acid and extracellular matrix. Our study established a reasonable method to generate pig pluripotent cells, which might be a new donor cell source for human neural disease therapy. © 2014 Society for Reproduction and Fertility.


Ying Y.,North East Agricultural University | Ying Y.,South China Agricultural University | Li J.,North East Agricultural University
Proceedings of the 2012 National Conference on Information Technology and Computer Science, CITCS 2012 | Year: 2012

Recently, abnormal changes of global climate, represented by earth warming, has become a common threat and challenge faced by the world, and has directly affectted the existence of the human society and its sustainable development. Kyoto Protocol allowed developed countries to provide developing countries capital and technology, in order to conduct forestation and reforestation carbon-sink program, utilize the carbon amount generated by the program to counteract emission conduct target in their countries. Therefore, forest carbon-sink transaction turned into an indirect emission reduction pattern that is widely practiced by various countries in the world. Now China doesn't have to undertake the compelling emission reduction duty, but along with the increasingly deep negotiation of the international climate, the reduction pressure becomes heavier and heavier. Therefore, developing the forest carbon-sink market transaction not only is beneficial to raise money, which is needed for forestation and increase the percentage of forest cover, but also can help to relieve the emission reduction pressure confronted by China. It has strategic significance to the sustainable development of the Chinese economy. This article discusses the theory basis to conduct forest carbon-sink market transaction, using SWOT analysis method to theoretically analyze the superiority, weakness, opportunity and challenge of the development of forest carbon-sink market transaction in China. At last, It provides some relevant countermeasures and suggestions. © 2012. The authors - Published by Atlantis Press.


Shan Z.-Y.,Harbin Medical University | Wu Y.-S.,Harbin Medical University | Shen X.-H.,Harbin Medical University | Li X.,Harbin Medical University | And 9 more authors.
Development Growth and Differentiation | Year: 2012

Parthenogenetic embryonic stem cells (PgES) might advance cell replacement therapies and provide a valuable in vitro model system to study the genomic imprinting. However, the differential potential of PgES cells was limited. It could result from relative low heterology of PgES cells compared with ES cells from fertilization (fES), which produce different expression of most imprinted genes. Here, we described the establishment of PgES cells by aggregating parthenogenetic embryos at the 8-cell stage (aPgES cells), which may increase heterozygy. We found that derivation of aPgES cells in association with an increased number of inner cell mass cells by aggregating was more efficient than that of PgES cells from a single parthenogenetic blastocyst. The aPgES cells have normal karyotype, stain positive for alkaline phosphatase, express high levels of ES cell markers and can differentiate into teratomas composed of the three germ layers. Moreover, compared with PgES cells, the more highly upregulated paternally expressed imprinted genes were observed in aPgES cells, the same change was not shown in aPg blastocysts. This suggested that the aggregation induced effect could modify the expression of paternally expressed imprinted genes. Our studies showed that aPgES cells, the expression of imprinted genes in which more closely resemble fES cells than PgES cells, would contribute to all organs and avoiding immuno-rejection, which may provide invaluable material for regeneration medicine. © 2012 The Authors. Development, Growth & Differentiation © 2012 Japanese Society of Developmental Biologists.


Ji G.,Nankai University | Ruan W.,China Agricultural University | Liu K.,Nankai University | Wang F.,Nankai University | And 12 more authors.
PLoS ONE | Year: 2013

Telomere reprogramming and silencing of exogenous genes have been demonstrated in mouse and human induced pluripotent stem cells (iPS cells). Pigs have the potential to provide xenotransplant for humans, and to model and test human diseases. We investigated the telomere length and maintenance in porcine iPS cells generated and cultured under various conditions. Telomere lengths vary among different porcine iPS cell lines, some with telomere elongation and maintenance, and others telomere shortening. Porcine iPS cells with sufficient telomere length maintenance show the ability to differentiate in vivo by teratoma formation test. IPS cells with short or dysfunctional telomeres exhibit reduced ability to form teratomas. Moreover, insufficient telomerase and incomplete telomere reprogramming and/or maintenance link to sustained activation of exogenous genes in porcine iPS cells. In contrast, porcine iPS cells with reduced expression of exogenous genes or partial exogene silencing exhibit insufficient activation of endogenous pluripotent genes and telomerase genes, accompanied by telomere shortening with increasing passages. Moreover, telomere doublets, telomere sister chromatid exchanges and t-circles that presumably are involved in telomere lengthening by recombination also are found in porcine iPS cells. These data suggest that both telomerase-dependent and telomerase-independent mechanisms are involved in telomere reprogramming during induction and passages of porcine iPS cells, but these are insufficient, resulting in increased telomere damage and shortening, and chromosomal instability. Active exogenes might compensate for insufficient activation of endogenous genes and incomplete telomere reprogramming and maintenance of porcine iPS cells. Further understanding of telomere reprogramming and maintenance may help improve the quality of porcine iPS cells. © 2013 Ji et al.


Xiong Z.-J.,Yunnan University | Xiong Z.-J.,Xinjiang Institute of Ecology and Geography | Zhang Y.-G.,Yunnan University | Zhang Y.-G.,Xinjiang Institute of Ecology and Geography | And 7 more authors.
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2013

A novel bacterium, designated YIM M13075T, was isolated from a sediment sample collected from the South China Sea. Growth occurred from 4 to 45 C (optimum 28 C), pH 6.0-11.0 (optimum pH 8.0). The strain formed yellow-cream colonies after 5 days incubation on TSA modified with 5 % NaCl medium at 28 C. Cells were Gram-positive, short rods and motile. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM M13075T was affiliated with the genus Geomicrobium (93.5 %). The strain YIM M13075 T contained meso-diaminopimelic acid in the cell wall. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The major fatty acids were iso-C15:0 and anteiso-C15:0. The predominant menaquinones were MK-7 and MK-6. The genomic DNA G+C content was 42.7 mol%. On the basis of the morphological and chemotaxonomic characteristics as well as genotypic data, strain YIM M13075T represents a novel species in the genus Geomicrobium, for which the name Geomicrobium sediminis sp. nov. is proposed. The type strain is YIM M13075T (=DSM 25540 T =JCM 18144T =CCTCC AB 2013245T). An emended description of the genus Geomicrobium is also proposed in the light of the new data. © 2013 Springer Science+Business Media Dordrecht.


Gravel V.,Wageningen University | Gravel V.,Agriculture and Agri Food Canada | Blok W.,Wageningen University | Wang H.,North East Agricultural University | And 7 more authors.
Agronomy for Sustainable Development | Year: 2010

Soil-bound intensive greenhouse production has been scrutinized for its sustainability due to contamination of ground water by over-fertilization resulting in leaching of nutrients. As environmental guidelines are becoming more restrictive worldwide, and especially in Europe, many greenhouse growers have converted to more sustainable production systems including rockwool culture with recycled water and organic cropping systems in soil. The increase in popularity of organic production systems has amplified the debate whether organically grown produce is healthier than conventional produce. So far, little is known about the variations in fruit quality associated with production systems for greenhouse grown tomatoes. Thus, two organic (organic fertilization with and without straw amendment) and three conventional tomato cropping systems (regular and increased nutrient solution in rockwool and regular fertilization in soil) were compared in order to evaluate differences in nutrient availability and effects on fruit quality over a three-year period. Three modern medium-sized round tomato cultivars and one old cultivar were compared. There were no significant interactions between cropping systems and cultivars, so that main effects of systems and cultivars could be evaluated. Fruit yields in the organic systems were similar to those obtained in the conventional soil-bound system, but 15% lower than in the regular rockwool system, even though nitrogen concentrations in soil were not limiting in any of the production systems. Frequent organic amendments resulted in higher soil contents in the organic system without straw than in the other soil-bound systems, indicating that the organic systems were not yet stable in terms of nutrient availability after three years. A fruit quality index, based on the contents of compounds such as lycopene, β-carotene and vitamin C, was similar in all cropping systems. The old cultivar had a significantly higher quality index, but a lower yield than the other cultivars. According to this study, high quality tomatoes can be obtained through proper adjustment of the quantity and the source of nitrogen fertilizers in organic and conventional cropping systems and the use of selected cultivars with a high nutrient use efficiency for organic systems. © 2010 INRA, EDP Sciences.


Meng X.,Zhejiang University of Technology | Ye Q.,Zhejiang University of Technology | Nie X.,Zhejiang University of Technology | Pan Q.,China Agricultural University | Jiang L.,North East Agricultural University
JAOCS, Journal of the American Oil Chemists' Society | Year: 2015

Partial least squares (PLS) is a commonly used (and sometimes misused) chemometric technique for calibrating Fourier transform infrared spectroscopy, and allows the analysis of a variety of quality parameters associated with edible oils. Peroxide value (PV) is a typical parameter of interest; however, developing a robust, optimal, and reliable calibration method can be a daunting task. This paper examines and compares the use of interval PLS as a tool to develop a PLS PV calibration method for a single-bounce attenuated total reflectance accessory relative to full spectrum PLS and experienced PLS, making use of correlation, variance, and pure component spectra. Using mixtures of fresh and oxidized oil covering a PV range of 1-20 meq/kg, backward interval PLS could systematically produce quality calibrations without the need to resort to experienced PLS. The experienced PLS requires a degree of spectral knowledge as well as diligent and tedious spectral examination, including largely unstructured iterative calibrations and cross-validations to improve calibration performance. The backward interval PLS is also better than the full spectrum PLS in terms of model performance. In addition, the general model developed could account for the errors caused by oil types. © 2015 AOCS.


Shan Z.-Y.,Harbin Institute of Technology | Wu Y.-S.,Harbin Institute of Technology | Li X.,Harbin Institute of Technology | Shen X.-H.,Harbin Institute of Technology | And 4 more authors.
Cellular Reprogramming | Year: 2014

Induced pluripotent stem cells (iPSCs) are usually generated by reprogramming somatic cells through transduction with a transcription factor cocktail. However, the low efficiency of this procedure has kept iPSCs away from the study of the clinical application of stem cell biology. Our research shows that continuous passage increases the efficiency of reprogramming. Compared with conventional method of establishment of iPSCs, more embryonic stem cell (ESC)-like clones are generated by continuous passage during early reprogramming. These inchoate clones, indistinguishable from genuine ESC clones, are closer to fully reprogrammed cells compared with those derived from classical iPSC induction, which increased the expression of pluripotent gene markers and the levels of demethylation of Oct4 and Nanog. These results suggested that full reprogramming is a gradual process that does not merely end at the point of the activation of endogenous pluripotency-associated genes. Continuous passage could increase the pluripotency of induced cells and accelerate the process of reprogramming by epigenetic modification. In brief, we have provided an advanced strategy to accelerate the reprogramming and generate more nearly fully reprogrammed iPSCs efficiently and rapidly. © Mary Ann Liebert, Inc.


Elbasit A.M.A.,University of Johannesburg | Elbasit A.M.A.,Desertification Research Institute | Ojha C.S.P.,Indian Institute of Technology Roorkee | Huang J.,Tottori University | And 4 more authors.
Journal of Food, Agriculture and Environment | Year: 2013

Soil water erosion is a well known cause of land degradation in the Chinese Loess Plateau. Many of its rivers, such as Yellow River are known to carry huge sediment loads. For proper management of sediment laden rivers, it is essential to identify those catchments which have high erosion potential. Such an assessment may be effectively used in watershed treatment to mitigate the effects of sediment erosion and is currently facilitated by a series of erosion indices. Rainfall erosivity is one of the major factors that control soil erosion in Loess Plateau, China. Several indices have been developed to quantify the rainfall erosivity; however, none of them was specifically developed for arid environments. In this study we examined the performance of several indices with different temporal-resolution of the required rainfall data inputs. Rainfall data were measured with 0.5 second logging interval in Liudaogou basin, Loess Plateau China. The monthly, daily, and 15-minute rainfall data were used to calculate the Fournier Index (IF), half month erosivity index (Mi), and monthly erosivity index (EI30). The relationship between the examined erosivity indices and monthly rainfall depth (Pi) was observed to be statistically significant. Power relationships between the EI30 and FI were able to represent the EI30 significantly (R2 = 0.77, P<0.001). However, the results also showed that the relationships are not suited to the intensified rainfall storms.


PubMed | North East Agricultural University and Harbin Medical University
Type: | Journal: Scientific reports | Year: 2016

Genome-wide transcriptome studies have identified thousands of long intergenic noncoding RNAs (lincRNAs), some of which play important roles in pre-implantation embryonic development (PED). Pig is an ideal model for reproduction, however, porcine lincRNAs are still poorly characterized and it is unknown if they are associated with porcine PED. Here we reconstructed 195,531 transcripts in 122,007 loci, and identified 7,618 novel lincRNAs from 4,776 loci based on published RNA-seq data. These lincRNAs show low exon number, short length, low expression level, tissue-specific expression and cis-acting, which is consistent with previous reports in other species. By weighted co-expression network analysis, we identified 5 developmental stages specific co-expression modules. Gene ontology enrichment analysis of these specific co-expression modules suggested that many lincRNAs are associated with cell cycle regulation, transcription and metabolism to regulate the process of zygotic genome activation. Futhermore, we identified hub lincRNAs in each co-expression modules, and found two lincRNAs TCONS_00166370 and TCONS_00020255 may play a vital role in porcine PED. This study systematically analyze lincRNAs in pig and provides the first catalog of lincRNAs that might function as gene regulatory factors of porcine PED.

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