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New Delhi, India

Sengupta J.,North DMC Medical College | Ghosh D.,All India Institute of Medical Sciences
Progress in Biophysics and Molecular Biology | Year: 2014

Implantation is a complex process which results in fixation of zona pellucida free blastocyst to the maternal uterine endometrium. In the human, it involves progesterone mediated preparation of endometrium, age- and stage-matched development of pre-implantation embryo, and interaction between embryo and endometrium. In the present essay, we present the case to explain why there is a necessity of undertaking multi-level, multi-scale integrative approach to deconstruct the succession process of endometrial development to the climax of implantation. © 2013 Elsevier Ltd. Source

Huppertz B.,Medical University of Graz | Ghosh D.,All India Institute of Medical Sciences | Sengupta J.,North DMC Medical College
Progress in Biophysics and Molecular Biology | Year: 2014

The placenta is an indispensable organ for intrauterine protection, development and growth of the embryo and fetus. It provides tight contact between mother and conceptus, enabling the exchange of gas, nutrients and waste products. The human placenta is discoidal in shape, and bears a hemo-monochorial interface as well as villous materno-fetal interdigitations. Since Peter Medawar's astonishment to the paradoxical nature of the mother-fetus relationship in 1953, substantial knowledge in the domain of placental physiology has been gathered. In the present essay, an attempt has been made to build an integrated understanding of morphological dynamics, cell biology, and functional aspects of genomic and proteomic expression of human early placental villous trophoblast cells followed by a commentary on the future directions of research in this field. © 2013 Elsevier Ltd. Source

Mahar S.,North DMC Medical College
Mymensingh medical journal : MMJ | Year: 2014

Ecliptaalba (Bhringraj) is an important traditional Indian medicinal plant reported to have antipyretic, anti-inflammatory, antihistaminic, hepatoprotective and anthelmintic effect well documented in literature. In the present study ethanolic leaf extract of eclipta alba (EEEA) was evaluated for its haemostatic activity by their ability to affect the screening test or modify the experimentally induced prolongation of test time on the basis of its folklore claim in normal, aspirin and heparin treated rabbits. The parameters recorded were BT, CT, PT and APTT & platelet count in all the three groups. The study was carried out in 3 parts in different doses 200,400,600 & 800mg/kg. Part-A-study of the haemostatic effect of Ethanolic extract of E. alba (EEEA) in graded doses on normal rabbits. Part-B-study of EEEA in aspirin treated rabbits. Part-C-study of EEEA in heparin treated rabbits. Tranexamic acid was used as the standard drug. Statistical analysis was performed using one way ANOVA & student t test. In normal rabbits EEEA high doses 800mg/k & 600mg/kg exhibited significant reduction in BT & CT (p<0.01). The same doses were found to decrease the aspirin induced increase in BT & heparin induced increase in CT in a significant manner (p<0.01). As decrease in CT might be due to liberation of some active principles from leaves of Ecliptaalba which demonstrated to have anti-haemorrhagic activity against snake bite not affecting any coagulation factor. No significant effect on PT, APPT and platelet count was observed. EEEA exhibited definite haemostatic effect in the above 3 sets of study. Phytochemical analysis and isolation of active principle is essential to carry on beneficial effects from laboratory bench to bedside of patients. Source

Srivastava A.,All India Institute of Medical Sciences | Sengupta J.,All India Institute of Medical Sciences | Sengupta J.,North DMC Medical College | Kriplani A.,All India Institute of Medical Sciences | And 2 more authors.
Reproductive Biology and Endocrinology | Year: 2013

Background: Several studies have indicated that human pre-implantation embryo-derived chorionic gonadotropin (hCG) may influence the implantation process by its action on human endometrial epithelial and stromal cells. Despite reports indicating that hCG acts on these cells to affect the production of several cytokines and growth factors (e.g., MIF, IGF-I, VEGF, LIF, IL-11, GMCSF, CXL10 and FGF2), our understanding of the integral influence of hCG on paracrine interactions between endometrial stromal and epithelial cells during implantation is very limited. Methods: In the present study, we examined the profile of 48 cytokines in the conditioned media of primary cell cultures of human implantation stage endometrium. Endometrial epithelial cells (group 1; n = 20), stromal cells (group 2; n = 20), and epithelial plus stromal cells (group 3; n = 20) obtained from mid-secretory stage endometrial samples (n = 60) were grown on collagen and exposed to different doses (0, 1, 10 and 100 IU/ml) of rhCG for 24 h in vitro. Immunochemical and qRT-PCR methods were used to determine cytokine profiles. Enrichment and process networks analyses were implemented using a list of cytokines showing differential secretion in response to hCG. Results: Under basal conditions, endometrial epithelial and stromal cells exhibited cell type-specific profiles of secreted cytokines. Administration of hCG (100 IU) resulted in significantly (P < 0.05) different cytokine secretion profiles indicative of macropinocytic transport (HGF, MCSF) in epithelial cells, signal transduction (CCL4, FGF2, IL-1b, IL-6, IL-17, VEGF) in stromal cells, and epithelial-mesenchymal transition (FGF2, HGF, IL-1b, TNF) in mixed cells. Overall, the administration of hCG affected cytokines involved in the immune response, chemotaxis, inflammatory changes, proliferation, cell adhesion and apoptosis. Conclusions: CG can influence the function of the endometrium during blastocyst implantation via its differential action on endometrial epithelial and stromal cells. CG may also affect complex paracrine processes in the different endometrial cell types. © 2013 Srivastava et al.; licensee BioMed Central Ltd. Source

Verma P.,North DMC Medical College | Namdeo C.,Ss Medical College
Indian Journal of Dermatology | Year: 2015

An endeavour to delineate the salient details of the treatment of head lice infestation has been made in the present article. Treatment modalities including over the counter permethrin and pyrethrin, and prescription medicines, including malathion, lindane, benzyl alcohol, spinosad are discussed. Salient features of alternative medicine and physical treatment modalities are outlined. The problem of resistance to treatment has also been taken cognizance of. Source

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