North China Petroleum Bureau General Hospital

Renqiu, China

North China Petroleum Bureau General Hospital

Renqiu, China
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Wang H.-G.,North China Petroleum Bureau General Hospital | Cao B.,North China Petroleum Bureau General Hospital | Zhang L.-X.,North China Petroleum Bureau General Hospital | Song N.,North China Petroleum Bureau General Hospital | And 5 more authors.
Oncology Reports | Year: 2017

The transcription factor Krüppel-like factor 2 (KLF2) has been shown to function as a tumor suppressor and regulate biological processes of cancer cells, such as cell growth, cell apoptosis and angiogenesis. However, the function and mechanism of KLF2 in colorectal cancer (CRC) is still unknown. In the present study, we show that the expression of KLF2 is diminished in a cohort of CRC cell lines. Also, KLF2 overexpression remarkably inhibits HCT116 and SW480 cell survival and proliferation. Moreover, cell death detection ELISA plus assay showed that KLF2 overexpression increased HCT116 cell proliferation. Caspase-3/7 activity also increased in HCT116 cells transfected with PcDNA3.1-KLF2. Further studies showed that KLF2 significantly suppresses the expression of Notch-1 and is dependent on the decline of the HIF-1-level. Most importantly, silencing Notch-1 expression or HIF-1-level both impair the action of KLF2 overexpression in CRC cells. Collectively, we demonstrated that KLF2 mediates CRC cell biological processes including cell growth and apoptosis via regulating the HIF-1-/Notch-1 signal pathway. These results indicated that KLF2 plays an important role in CRC and provided novel insight on the function of KLF2 in tumor progression.


Lin Q.,North China Petroleum Bureau General Hospital | Gao X.-S.,Beijing University First Hospital | Qiao X.-Y.,Hebei Medical University | Liu C.-X.,Beijing University First Hospital | And 5 more authors.
Oncology Letters | Year: 2011

This study aimed to compare the efficacy of late--course accelerated hyperfractionation radiotherapy (LAFR) and concurrent chemoradiotherapy (CRT) in patients with esophageal carcinoma and to evaluate the side effects of the two treatments. A total of 22 patients with primary esophageal squamous cell carcinoma were prospectively treated with LAFR, while 25 patients, during the same period, served as the control group and received CRT. The 22 patients in the LAFR group received conventional fractionated radiotherapy of 30 Gy over a 3-week period (5 daily fractions of 2.0 Gy per week), followed by accelerated hyperfractionated radiotherapy of 30 Gy for 2 weeks (twice daily, 1.5 Gy per fraction, with a minimal interval of 6 h between fractions, 10 fractions per week). The 25 patients in the CRT group received conventional fractionated radiotherapy of 50 Gy for 5 weeks, with 5 daily fractions of 2.0 Gy per week. Chemotherapy was started on the first day of irradiation (cisplatin 52.5 mg/m2 on Day 1 and 5-fluorouracil 700 mg/m2 on Days 1-5, repeated four times every 28 days). The median survival time in the LAFR and CRT groups were noted to be 17 and 21 months, respectively. The 1-- and 2--year overall survival rates were 63.6 and 31.6% in the LAFR group and 76 and 57.4% in the CRT group (χ2=1.670; P=0.196). The median local control in the LAFR group was 17 months, while that in the CRT group was not determined. The 1- and 2-year local control rates were 54.5 and 39% in the LAFR group while those in the CRT group were 82.2 and 66.1% (χ2=3.527; P=0.060). The overall survival and local control rates of the LAFR group were lower than those of the CRT group, although the difference was not significant. The metastasis rates of the two groups were also not significantly different (χ2=0.030; P=0.862). Both acute and late adverse events in the two groups were tolerated. The side effects, including hematological toxicities, severe nausea and vomiting, and severe anorexia were significantly less in the LAFR group than those in the CRT group (P<0.05). In this small--sample exploratory study, the overall survival and local control rates were lower with LAFR than with CRT, but the difference was not significant. Moreover, LAFR was found to have fewer side effects and be more cost--effective compared to CRT. The long--term effects on LAFR survival should be evaluated in a phase III clinical trial.


Liu R.,Nanjing Southeast University | Yang M.,Nanjing Southeast University | Meng Y.,North China Petroleum Bureau General Hospital | Liao J.,Nanjing Southeast University | And 4 more authors.
PLoS ONE | Year: 2013

Recent studies have demonstrated the possible function of miR-139-5p in tumorigenesis. However, the exact mechanism of miR-139-5p in cancer remains unclear. In this study, the association of miR-139-5p expression with esophageal squamous cell carcinoma (ESCC) was evaluated in 106 pairs of esophageal cancer and adjacent non-cancerous tissue from ESCC patients. The tumor suppressive features of miR-139-5p were measured by evaluating cell proliferation and cell cycle state, migratory activity and invasion capability, as well as apoptosis. Luciferase reporter assay and Western blot analysis were performed to determine the target gene regulated by miR-139-5p. The mRNA level of NR5A2, the target gene of miR-139-5p, was determined in ESCC patients. Results showed that reduced miR-139-5p level was associated with lymph node metastases of ESCC. MiR-139-5p was investigated to induce cell cycle arrest in the G0/G1 phase and to suppress the invasive capability of esophageal carcinoma cells by targeting the 3′UTR of oncogenic NR5A2. Cyclin E1 and MMP9 were confirmed to participate in cell cycle arrest and invasive suppression induced by NR5A2, respectively. Pearson correlation analysis further confirmed the significantly negative correlation between miR-139-5p and NR5A2 expression. The results suggest that miR-139-5p exerts a growth- and invasiveness-suppressing function in human ESCCs, which demonstrates that miR-139-5p is a potential biomarker for early diagnosis and prognosis and is a therapeutic target for ESCC. © 2013 Liu et al.


Guo Z.-J.,North China Petroleum Bureau General Hospital | Lin Q.,North China Petroleum Bureau General Hospital | Liu H.-T.,North China Petroleum Bureau General Hospital | Lu J.-Y.,North China Petroleum Bureau General Hospital | And 6 more authors.
Acta Radiologica | Year: 2013

Background: Using computed tomography (CT) to rapidly and accurately quantify pleural effusion volume benefits medical and scientific research. However, the precise volume of pleural effusions still involves many challenges and currently does not have a recognized accurate measuring. Purpose: To explore the feasibility of using 64-slice CT volume-rendering technology to accurately measure pleural fluid volume and to then analyze the correlation between the volume of the free pleural effusion and the different diameters of the pleural effusion. Material and Methods: The 64-slice CT volume-rendering technique was used to measure and analyze three parts. First, the fluid volume of a self-made thoracic model was measured and compared with the actual injected volume. Second, the pleural effusion volume was measured before and after pleural fluid drainage in 25 patients, and the volume reduction was compared with the actual volume of the liquid extract. Finally, the free pleural effusion volume was measured in 26 patients to analyze the correlation between it and the diameter of the effusion, which was then used to calculate the regression equation. Results: After using the 64-slice CT volume-rendering technique to measure the fluid volume of the self-made thoracic model, the results were compared with the actual injection volume. No significant differences were found, P = 0.836. For the 25 patients with drained pleural effusions, the comparison of the reduction volume with the actual volume of the liquid extract revealed no significant differences, P = 0.989. The following linear regression equation was used to compare the pleural effusion volume (V) (measured by the CT volume-rendering technique) with the pleural effusion greatest depth (d): V = 158.16 × d 2 116.01 (r = 0.91, P = 0.000). The following linear regression was used to compare the volume with the product of the pleural effusion diameters (l × h × d): V = 0.56 × (l × h × d) + 39.44 (r = 0.92, P = 0.000). Conclusion: The 64-slice CT volume-rendering technique can accurately measure the volume in pleural effusion patients, and a linear regression equation can be used to estimate the volume of the free pleural effusion.


Guo Z.-J.,North China Petroleum Bureau General Hospital | Lin Q.,North China Petroleum Bureau General Hospital | Zi X.-R.,North China Petroleum Bureau General Hospital | Xu Q.,North China Petroleum Bureau General Hospital | And 7 more authors.
Radiologia Medica | Year: 2015

Conclusion: The higher the shock index and the ratio of false/true lumen are, the greater the transportation risk for AD patients. The shock index and the ratio of false/true lumen proved to be essential clinical and radiological indices for assessing the transportation risk of AD patients.Materials and methods: Thirty-six cases of AD confirmed with 64-slice volumetric CT (VCT) (18 cases of Stanford type A and 18 cases of type B) were divided into a high-risk group (14 cases, six Stanford type A and eight type B) and a low-risk group (22 cases, 12 Stanford type A and 10 type B) according to the modified Early Warning Score. The shock index (ratio of heart rate to systolic blood pressure) and measured CTA parameters were compared between the high-risk group and the low-risk group, and the correlation between the measured CTA parameters and shock index was analysed.Results: The shock index and ratio of false/true lumen were compared between Stanford type A and type B, and no statistically significant differences were found. The shock index and ratio of false/true lumen were compared between the high-risk group and low-risk group, revealing a statistically significant difference (p < 0.05). Moreover, a significant linear correlation was found between the ratio of false/true lumen and the shock index (r = 0.691; p = 0.001).Background: Aortic dissection (AD) is a serious, life-threatening disease. It is currently crucial for AD patients to be transferred to a specialised hospital in a safe and timely manner. For this reason, the search for clinical and imaging changes related to transportation risk is becoming increasingly important.Purpose: The transportation risks of AD patients were assessed by studying the correlation between computed tomography angiography (CTA) parameters and shock index. © 2014, Italian Society of Medical Radiology.


Guo Z.-J.,North China Petroleum Bureau General Hospital | Chen Y.-F.,North China Petroleum Bureau General Hospital | Zhang Y.-H.,North China Petroleum Bureau General Hospital | Meng F.-J.,North China Petroleum Bureau General Hospital | And 6 more authors.
Abdominal Imaging | Year: 2011

Aim: To explore multi-slice spiral CT (MSCT) virtual endoscopy (CTVE) in the detection of Vater's ampulla lesions. Methods: In addition to 30 healthy volunteers, 18 cases of common bile duct stones, and 7 cases of ampullary carcinoma were scanned with MSCT including virtual endoscopy (VE) reconstruction. Patterns of the duodenal papilla were then observed, and its size was measured. Results: Reconstructed images of CTVE in the volunteers showed that the normal type of the duodenal papilla was nodular in 16 cases, V-shaped in 8 cases, and Y-shaped in 6 cases. Its mean diameter was 0.84 ± 0.17 cm in the healthy volunteers; in patients with common bile duct stones of nodular type, mean diameter was 1.72 ± 0.32 cm. In ampullary cancer patients with an irregular protruded type, its diameter was 2.30 ± 0.85 cm, Overall the mean differences between the groups were statistically significant (P < 0.001). Conclusion: CTVE is a convenience, no-wound, and precise clinical examination mode utilizing which the shape of duodenal papilla can be observed, and size of the latter can be measured. © Springer Science+Business Media, LLC 2010.


PubMed | North China Petroleum Bureau General Hospital
Type: Journal Article | Journal: La Radiologia medica | Year: 2015

Aortic dissection (AD) is a serious, life-threatening disease. It is currently crucial for AD patients to be transferred to a specialised hospital in a safe and timely manner. For this reason, the search for clinical and imaging changes related to transportation risk is becoming increasingly important.The transportation risks of AD patients were assessed by studying the correlation between computed tomography angiography (CTA) parameters and shock index.Thirty-six cases of AD confirmed with 64-slice volumetric CT (VCT) (18 cases of Stanford type A and 18 cases of type B) were divided into a high-risk group (14 cases, six Stanford type A and eight type B) and a low-risk group (22 cases, 12 Stanford type A and 10 type B) according to the modified Early Warning Score. The shock index (ratio of heart rate to systolic blood pressure) and measured CTA parameters were compared between the high-risk group and the low-risk group, and the correlation between the measured CTA parameters and shock index was analysed.The shock index and ratio of false/true lumen were compared between Stanford type A and type B, and no statistically significant differences were found. The shock index and ratio of false/true lumen were compared between the high-risk group and low-risk group, revealing a statistically significant difference (p<0.05). Moreover, a significant linear correlation was found between the ratio of false/true lumen and the shock index (r=0.691; p=0.001).The higher the shock index and the ratio of false/true lumen are, the greater the transportation risk for AD patients. The shock index and the ratio of false/true lumen proved to be essential clinical and radiological indices for assessing the transportation risk of AD patients.

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