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Wang F.,Ford Motor Company | He Q.,Ford Motor Company | Sun Y.,Ford Motor Company | Sun Y.,North China Coal Medical College | And 2 more authors.

Premenopausal women have less cardiovascular disease and lower cardiovascular morbidity and mortality than men the same age. Our previous studies showed that female mice have lower mortality and better preserved cardiac function after myocardial infarction. However, the precise cellular and molecular mechanisms responsible for such a sex difference are not well established. Using cultured adult mouse cardiomyocytes, we tested the hypothesis that the survival advantage of females stems from activated estrogen receptors and Akt survival signaling pathways. Adult mouse cardiomyocytes were isolated from male and female C57BL/6J mice and treated with hydrogen peroxide (100 μmol/L) for 30 minutes. Cell survival was indicated by rod ratio (rod shaped cells:total cells), cell death by lactate dehydrogenase release, and positive staining of annexin-V (a marker for apoptosis) and propidium iodide (a marker for necrosis). In response to hydrogen peroxide, female adult mouse cardiomyocytes exhibited a higher rod ratio, lower lactate dehydrogenase release, and fewer Annexin-V-positive and propidium iodide-positive cells compared with males. Phospho-Akt was greater in females both at baseline and after hydrogen peroxide stimulation. The downstream molecule of Akt, phosphor-GSK-3β (inactivation), was also higher, whereas caspase 3 activity was lower in females in response to hydrogen peroxide. Bcl-2 did not differ between sexes. Estrogen receptor-α was the dominant isoform in females, whereas estrogen receptor-β was low but similar in both sexes. Our findings demonstrate that female adult mouse cardiomyocytes have a greater survival advantage when challenged with oxidative stress-induced cell death. This may be attributable to activation of Akt and inhibition of GSK-3β and caspase 3 through an estrogen receptor-α-mediated mechanism. Copyright © 2010 American Heart Association. All rights reserved. Source

Peng H.B.,North China Coal Medical College
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases

To study the proliferation effect of the AM supernatant incubated activation of p38 mitogen activated protein kinases (p38MAPK) signal transduction pathway in human embryonic lung fibroblasts, and to participate in the development of fibrosis in silicosis. The silicotic alveolar macrophages were collected by bronchoalveolar lavage and incubated in vitro in the DMEM medium containing SiO 2 (50 μg/ml) and DMEM medium without SiO 2 for 18 h. Then the AM supernatant incubated for 18 h was collected. HELFs were isolated by organize paste block method, and incubated with AM supernatants. HELFs were divided into four groups: blank control groups, AM groups, SiO 2 + AM groups, SB203580 + SiO 2 + AM groups. The proliferation in the HELF was detected with MTT method and Flow cytometry. The proliferation in the HELF acted with the conditioned AM supernatant fluid were more than blank control groups, AM groups and SB203580 + SiO 2 + AM groups [average optical density: (0.48 ± 0.03) vs (0.29 ± 0.01), (0.38 ± 0.02), (0.33 ± 0.03)], the values with MTT method were statistically different (P < 0.05); Proliferous index with flow cytometry in SiO 2 + AM groups (18.12 ± 0.82) was bigger than blank control groups (9.24 ± 0.48), AM groups (14.76 ± 0.43) and SB203580 + SiO 2 + AM groups (11.71 ± 0.70) and the values were statistically different(P < 0.05). The AM supernatant stimulated by silicon dioxide can accelerate the proliferation in the HELF by activation of p38MAPK signal transduction pathway. Source

Zheng S.,North China Coal Medical College | Zheng S.,Indiana University | Hodges K.B.,Indiana University | Sun Y.,North China Coal Medical College | And 7 more authors.
Anticancer Research

KISS1 and matrix metalloproteinase-9 (MMP-9) may play important roles as metastasis suppressor and metastasis promoter genes, respectively, in a variety of malignancies. However, there is little information about their possible roles in non-small cell lung cancer (NSCLC). The goals of this study were to determine the mRNA and protein expressions of KISS1 and MMP-9 in NSCLC and their relations to metastasis and prognosis. The mRNA and protein expressions of KISS1 and of MMP-9 protein were detected by in situ hybridization and immunohistochemistry respectively in 85 cases of NSCLC, and their matched lymph node metastases. Expressions of KISS1 mRNA and protein were significantly higher in low TNM stages of NSCLC (I-II) compared to more advanced stages (HI-IV) (p<0.05). Moreover, in advanced TNM stages, cases without metastasis had higher KISS1 gene expression compared to those with lymph node metastasis (p<0.05). In contrast, MMP-9 expression was higher in stage IH-IV NSCLC cases compared to stage I-II tumors (p<0.05) and higher in NSCLC cases with metastasis than those without metastasis (p<0.05). There was negative correction between KISS1 and MMP-9 protein expression (p<0.01). The 5-year survival rate in cases with higher KISS1 protein expression was significantly higher than in those with low expression (20.9 vs. 2.4%, p<0.01). However, the 5-year survival rate of patients with high MMP-9 protein expression were lower than those with low expression (19 vs. 4.7%, p<0.05). Our data suggest that KISS1 and MMP-9 may serve as potential prognostic and therapeutic markers in lung cancer. Source

Wang G.,North China Coal Medical College | Wu J.,North China Coal Medical College | Su Y.,North China Coal Medical College
Advanced Materials Research

Ant colony algorithm is a kind of effective combinatorial optimization problem solving algorithm has been increasingly, thorough research, and gradually get used. Ant colony algorithm is a set of parameters, the algorithm, a lack of adequate experiences often. The paper has put forward a single genetic character of ant colony algorithm. Will the ant colony algorithm each search results as the initial population, single genetic improvement, for the shortest route optimization. In the traveling salesman problem of the experiments prove the effectiveness of the proposed algorithm. © (2011) Trans Tech Publications. Source

Wang J.,North China Coal Medical College | Wang G.,North China Coal Medical College | Wu J.,North China Coal Medical College | Su Y.,North China Coal Medical College
Applied Mechanics and Materials

Artificial neural network is based on human brain structure and operational mechanism based on knowledge and understanding of its structure and behavior of simulated an engineering system. BP artificial neural network is an important component of neural networks, as it can on the linear or nonlinear multivariable without preconditions in the case of statistical analysis, with the traditional statistical methods, analysis of the variables need to be consistent with certain conditions compared to its own advantage. The BP neural network does not need the precise mathematical model, does not have any supposition request to the material itself. Its processing non-linear problem's ability is stronger than traditional statistical methods. This article uses two groups of data to establish the BP neural network model separately, and carries on the comparison to the model fitting ability and the forecast performance, discovered BP neural network when data distribution relative centralism fits ability, forecasts the stable property. But the predictive ability is unable in the discrete data application to achieve anticipated ideally. © (2011) Trans Tech Publications. Source

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