Kita-ku, Japan
Kita-ku, Japan

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Terada K.,North | Horinouchi T.,North | Fujioka Y.,Hokkaido University | Higashi T.,North | And 9 more authors.
Journal of Biological Chemistry | Year: 2014

Two types of G protein-coupled receptors for endothelin-1 (ET-1), ET type A receptor (ETAR) and ETBR, closely resemble each other, but upon ET-1 stimulation, they follow totally different intracellular trafficking pathways; ETAR is recycled back to plasma membrane, whereas ETBR is targeted to lysosome for degradation. However, the mechanisms for such different fates are unknown. Here we demonstrated that ETBR but not ETAR was ubiquitinated on the cell surface following ET-1 stimulation and that ETBR was internalized and degraded in lysosome more rapidly than ETAR. The mutant ETBR (designated "5KR mutant") in which 5 lysine residues in the C-tail were substituted to arginine was not ubiquitinated, and its rates of internalization and degradation after ET-1 stimulation became slower, being comparable with those of ETAR. Confocal microscopic study showed that following ET-1 stimulation, ETAR and 5KR mutant of ETBR were co-localized mainly with Rab11, a marker of recycling endosome, whereas ETBR was co-localized with Rab7, a marker of late endosome/lysosome. In the 5KR mutant, ET-1-induced ERK phosphorylation and an increase in the intracellular Ca2+ concentration upon repetitive ET-1 stimulation were larger. A series of ETBR mutants (designated "4KR mutant"), in which either one of 5 arginine residues of the 5KR mutant was reverted to lysine, were normally ubiquitinated, internalized, and degraded, with ERK phosphorylation being normalized. These results demonstrate that agonist-induced ubiquitination at either lysine residue in the C-tail of ETBR but not ETAR switches intracellular trafficking from recycling to plasma membrane to targeting to lysosome, causing decreases in the cell surface level of ETBR and intracellular signaling. © 2014 by The American Society for Biochemistry and Molecular Biology Inc.


News Article | November 3, 2016
Site: www.prweb.com

Sunday, October 30 marked North-West College's Fall Commencement Ceremony. Held at the Pasadena Civic Auditorium, the event offered family and friends, along with College Team Members, the opportunity to congratulate 422 graduates as they celebrated the culmination of their career training. "Soon, you'll be experiencing the gratification of entering the World of Work with not just having a job, but a career," President Mitchell Fuerst shared with the graduates. "You should be proud of yourselves as every person in this auditorium today is proud of you." Students crossed the stage to receive their diplomas by program, with individuals representing the College's Medical Assistant, Dental Assistant, Vocational Nurse, Eyecare Specialist, Massage Therapist, Surgical Technologist, and Pharmacy Technician programs, among others. As part of their program, every student participated in classroom-based learning along with hands-on training opportunities. In addition, every program includes a clinical externship component, allowing students to practice their skills in real-world environments prior to graduation. This dynamic combination produces well-equipped graduates who are prepared for the rigors of the health care field. El Monte City Councilman and Keynote Speaker Juventino "J" Gomez addressed the graduates during the ceremony, saying, "This is such an important beginning to your life—it's the beginning of a new life. You have committed to bettering yourselves, and that's exactly what you've done by attending North-West College." Guests and graduates also enjoyed performances by both vocalist Scott A. People and jazz singer and trumpeter Ilya Serov. In addition, social media enhanced the overall Commencement experience. For the first time, the College streamed the ceremony live on Facebook, giving those unable to attend a chance to watch Commencement remotely. Custom Snapchat filters also added fun for guests and graduates alike. To watch the full Commencement Ceremony video, visit facebook.com/northwestcollege/. Interested in learning more about North-West College's programs? Please contact Nancy Aguillon for more information today! Founded in 1966, North-West College has been committed to training individuals to enter and advance in the health care field for 50 years. A leader in allied health education, the College offers 12 short-term programs at seven campuses throughout Southern California, including West Covina, Pomona Valley, Pasadena, Glendale, Riverside, Santa Ana, and Long Beach. Accredited by the Accrediting Commission of Career Schools and Colleges (ACCSC) and approved by the Bureau for Private Postsecondary Education (BPPE), North-West College offers a supportive educational environment for those ready to start a health care career. To date, the College has graduated more than 45,000 students—individuals who have gone on to raise the standard of excellence at health care organizations of all types. For more information about North-West College and its programs, visit http://www.nw.edu.

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