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Yang P.,Tianjin University | Zheng Q.-L.,Nordic Bioscience Beijing Ltd. | Wang J.-F.,Tianjin University | Huang J.-X.,Tianjin University | And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Allogenic islet transplantation for type 1 diabetes is limited by shortage of donor islet cells and immune suppression, bioengineered islet-like clusters developed from autogenic stem cells might benefit diabetic patients by immunorejection free and off-shelf cellular treatment. OBJECTIVE: To investigate whether adipose tissue from adults have the potential to differentiate into insulin secreting, glucose responsive, functional islet-like clusters and to develop a practical approach for islet bioengineering in vitro. METHODS: Human adipose stem cells were isolated, cultured and expanded from human subcutaneous adipose tissue. Conophylline, a new plant derived inducer, in combination with other factors were used to induce human adipose stem cells into islet-like clusters and their efficiencies for islet-like clusters generation were compared. Differentiated cells were identified by tissue specific surface marker staining, reverse transcription-PCR and immunocytochemistry at protein and mRNA level. Finally, insulin secretion of cell clusters in different glucose concentrations was tested with enzyme-linked immunosorbent assay. RESULTS AND CONCLUSION: Multi potential adipose stem cells were cultured and expanded from adult subcutaneous adipose tissues. Those stem cells were conveniently differentiated into glucose responsive and insulin-secreting functional islet-like clusters. Conophylline in combination with Nicotinamide have the best efficiency of differentiating human adipose stem cells into islet cells. Source

Liang H.,Shandong University | Liu P.,Shandong University | Liu P.,Nordic Bioscience Beijing Ltd. | Wang Y.,Shandong University | And 2 more authors.
Phytomedicine | Year: 2011

The neuronal damage following cerebral ischemia is a serious risk to stroke patients. The aim of this study was to investigate the neuroprotective effects of alkaloid extract from Leonurus heterophyllus (LHAE) on cerebral ischemic injury. After 24 h of reperfusion following ischemia for 2 h induced by middle cerebral artery occlusion (MCAO), some rats were intraperitoneally administered different doses of LHAE (3.6, 7.2, 14.4 mg/kg, respectively). Neurological examination was measured in all animals. Infarct volume, myeloperoxidase (MPO) activity, levels of nitrate/nitrite metabolite (NO) and apoptosis ratio of nerve fiber in brain were determined. The results showed that LHAE at 7.2 mg/kg or 14.4 mg/kg exerted significantly decreasing neurological deficit scores and reducing the infarct volume on rats with focal cerebral ischemic injury (p < 0.05). At those dose, the MPO content were significantly decreased in ischemic brain as compared with model group (p < 0.05). LHAE at 14.4 mg/kg significantly decreased the NO level compared with the model group (p < 0.05). In addition, LHAE significantly decreased the apoptosis ratio of nerve fiber compared with the model group (p < 0.05). This study suggests that LHAE may be used for treatment of ischemic stroke as a neuroprotective agent. Further studies are warranted to assess the efficacy and safety of LHAE in patients. © 2011 Elsevier GmbH. Source

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