Noor Research and Educational Institute

Tehrān, Iran

Noor Research and Educational Institute

Tehrān, Iran

Time filter

Source Type

Yazdanpanah H.,Shahid Beheshti University of Medical Sciences | Yazdanpanah H.,Iranian Ministry of Health and Medical Education | Shafaati A.,Shahid Beheshti University of Medical Sciences | Foroutan S.M.,Shahid Beheshti University of Medical Sciences | And 5 more authors.
Iranian Journal of Pharmaceutical Research | Year: 2014

The occurrence of deoxynivalenol (DON) in retail foods in Tehran (Iran) was determined using high-performance liquid chromatography technique and immunoaffinity column as the clean-up step. A method was validated for analysis of DON in rice, bread, puffed corn snack and wheat flour. The average recoveries and precision (RSD) for DON in different foods ranged 84.2-93.1% and 2.9-12.0%, respectively. A survey of DON was performed on the 72 samples of rice, bread, puffed corn snack, and wheat flour collected from Tehran retail market. The data showed that 10 samples (13.9%) out of 72 samples were contaminated with DON with the maximum level of 368.7 ng/g. The samples had contamination level lower than the maximum tolerated level of DON in foods in Iran. The total intake of DON was under the provisional maximum tolerable daily intake set for DON by the JECFA. © 2014 by School of Pharmacy.


Yazdanpanah H.,Shahid Beheshti University of Medical Sciences | Yazdanpanah H.,Iranian Ministry of Health and Medical Education | Zarghi A.,Shahid Beheshti University of Medical Sciences | Shafaati A.R.,Shahid Beheshti University of Medical Sciences | And 4 more authors.
Iranian Journal of Pharmaceutical Research | Year: 2012

Zearalenone (ZEA) mycotoxin is a potent estrogenic metabolite. It is the primary toxin causing infertility, abortion or other breeding problems. A HPLC method was validated for ZEA in foods using a monolithic column with sample clean-up on an immunoaffinity column. A certified reference material (CRM) from FAPAS (UK) was analyzed. A survey of ZEA was performed on the 72 samples of rice, bread, puffed corn snack and wheat flour collected from Tehran retail market. The average recovery and coefficient of variation in different foods ranged 92.7-107.1 and 4.9-13.8%, respectively. The amount of ZEA in corn CRM was in the acceptable range of FAPAS. The limit of quantification was 3 ng/g for rice, bread and wheat flour and 2.7 ng/g for puffed corn snack. The retention time of zearalenone was 2.6 min. All samples had contamination level lower than the maximum tolerated level of ZEA in foods in Iran. The mean intake of ZEA from all samples was much lower than the tolerable daily intake estimated by JECFA. This is the first survey on ZEA contamination in bread and rice in Iran as well as the first study on exposure assessment of Tehran population to ZEA. © 2012 by School of Pharmacy.


Yazdanpanah H.,Shahid Beheshti University of Medical Sciences | Zarghi A.,Shahid Beheshti University of Medical Sciences | Shafaati A.R.,Shahid Beheshti University of Medical Sciences | Foroutan S.M.,Shahid Beheshti University of Medical Sciences | And 4 more authors.
Iranian Journal of Pharmaceutical Research | Year: 2013

A high performance liquid chromatographic method was developed for determination of aflatoxin B1 (AFB1) in foods using a monolithic column with sample clean up on an immunoaffinity column. The method was validated for analysis of AFB1 in rice, bread, puffed corn snack, wheat flour and peanut samples. The average recoveries for AFB1 in different foods ranged from 94.4 to 102.5% with the coefficient of variation lower than 10% for all foods. Limit of detection was 0.01 ng/g. A survey of AFB1 was performed on 90 samples collected from Tehran retail market in June 2005. The results showed that none of the bread and wheat flour samples were contaminated with AFB1. The mean AFB1 levels in rice, puffed corn snack and peanut samples were 4.17, 0.11, and 1.97 ng/g, respectively. The level of contamination of 3 samples (one rice sample and two peanuts samples) to AFB1 was found to be higher than 5 ng/g. Although all food samples had mean concentration of AFB1 below the maximum tolerated level in Iran, the mean intake of AFB1 from rice was estimated 3.49 times higher than the guidance value of 1 ng AFB1/Kg body weight/day. Therefore, it is strongly recommended to monitor AFB1 in foods, especially in rice, in Iran. This is the first study on exposure assessment of Iranian population to AFB1. © 2013 by School of Pharmacy.


Foroutan S.M.,Shahid Beheshti University of Medical Sciences | Zarghi A.,Shahid Beheshti University of Medical Sciences | Shafaati A.,Shahid Beheshti University of Medical Sciences | Madadian B.,Noor Research and Educational Institute | Abolfathi F.,Noor Research and Educational Institute
Iranian Journal of Pharmaceutical Research | Year: 2013

A rapid, sensitive and reproducible HPLC method using amperometric detector was developed and validated for the analysis of clarithromycin in human plasma. The separation was achieved on a monolithic silica column (MZ- C8 125×4.0 mm) using acetonitrile- methanol-potassium dihydrogen phosphate buffer (40:6:54,v/v), with pH of 7.5, as the mobile phase at a flow rate of 1.5 mL/min. The assay enables the measurement of clarithromycin for therapeutic drug monitoring with a minimum quantification limit of 20 ng/mL. The method involves simple, protein precipitation procedure and analytical recovery was complete. The calibration curve was linear over the concentration range of 0.1-6 μg/mL. The coefficients of variation for inter-day and intra-day assay were found to be less than 6%. This method was used in bioequivalency and pharmacokinetic studies of the test (generic) product 2 × 500 mg clarithromycin tablets, with respect to the reference product. © 2013 by School of Pharmacy.

Loading Noor Research and Educational Institute collaborators
Loading Noor Research and Educational Institute collaborators