Non Public Health Care Unit Ovum Reproduction and Andrology

Lublin, Poland

Non Public Health Care Unit Ovum Reproduction and Andrology

Lublin, Poland
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Szymona-Paklowska K.,Catholic University of Lublin | Kraczkowski J.J.,Medical University of Lublin | Janowski K.,University of Warsaw | Steuden S.,Catholic University of Lublin | And 4 more authors.
Przeglad Menopauzalny | Year: 2014

Urinary incontinence (UI) is one of the most common diseases in women. It involves uncontrolled leaking of urine through the urethra. UI incidence depends on age and in certain age groups it can affect up to 60% of the female population. The symptoms can be persistent and due to their embarrassing nature they can lead to significant deterioration of quality of life and psychological functioning. The aim of this study was to investigate the relationship between the quality of life and selected aspects of functioning. Seventy female outpatients of a public clinic in Nałȩczów, Poland, were examined, and the following methods were used: the Urinary Incontinence Life Quality Scale (Szymona-Pałkowska, Kraczkowski), Disease-Related Appraisal Scale (Janowski, Steuden), Coping Inventory for Stressful Situations (Endler, Parker), Disease-Related Social Support Scale (Brachowicz, Janowski, Sadowska), and the Knowledge Appraisal Scale for Urinary Incontinence (Szymona- Pałkowska, Kraczkowski). The obtained results suggest that the strongest predictors for the quality of life with UI would be: perception of the disease as an injustice and a challenge (a reverse relationship) and an avoidance-oriented style of coping with stress through companionship seeking (a positive relationship). The duration of the disease and the level of related knowledge proved to be crucial in the analysis of one dimension, i.e. distance to the symptoms.


PubMed | University of Warsaw, Catholic University of Lublin, Public Health Care Unit, Medical University of Lublin and Non Public Health Care Unit Ovum Reproduction and Andrology
Type: Journal Article | Journal: Przeglad menopauzalny = Menopause review | Year: 2015

Urinary incontinence (UI) is one of the most common diseases in women. It involves uncontrolled leaking of urine through the urethra. UI incidence depends on age and in certain age groups it can affect up to 60% of the female population. The symptoms can be persistent and due to their embarrassing nature they can lead to significant deterioration of quality of life and psychological functioning. The aim of this study was to investigate the relationship between the quality of life and selected aspects of functioning. Seventy female outpatients of a public clinic in Naczw, Poland, were examined, and the following methods were used: the Urinary Incontinence Life Quality Scale (Szymona-Pakowska, Kraczkowski), Disease-Related Appraisal Scale (Janowski, Steuden), Coping Inventory for Stressful Situations (Endler, Parker), Disease-Related Social Support Scale (Brachowicz, Janowski, Sadowska), and the Knowledge Appraisal Scale for Urinary Incontinence (Szymona-Pakowska, Kraczkowski). The obtained results suggest that the strongest predictors for the quality of life with UI would be: perception of the disease as an injustice and a challenge (a reverse relationship) and an avoidance-oriented style of coping with stress through companionship seeking (a positive relationship). The duration of the disease and the level of related knowledge proved to be crucial in the analysis of one dimension, i.e. distance to the symptoms.


PubMed | Medical University of Lublin, Center of Oncology of Poland and Non Public Health Care Unit Ovum Reproduction and Andrology
Type: Journal Article | Journal: Przeglad menopauzalny = Menopause review | Year: 2015

The perimenopausal age is a time of many changes in womens health. Changes in womens health affect all spheres of life, because health is not merely the absence of disease or infirmity but full psychological, physical and social well-being. Presentation and comparison of the biopsychosocial functioning of women of different races and nationalities in perimenopause, identification of the most common menopause symptoms occurring among women and their needs.Work supported with research examples. Using the PubMed database, the medical literature was searched for works that contain the key words menopause and race or ethnicity published between 1996 and 2013 and available in English. Literature in Polish is a supplementary issue.Various demographic processes taking place in the countries of Africa, North and South America, Asia and Europe are the basis for observing the situation in perimenopausal women in these countries. Caucasian women living in North America and Europe most often experience the negative symptoms of menopause, hindering daily functioning, and women living in urbanized countries in Asia best assess their health condition, both psychosocial and physical.Biopsychosocial functioning of women varies among countries. Developing countries should be given the necessary support and financial information to ensure the health and quality of life in perimenopausal women. In most countries of the world there has been observed the need to promote womens health, particularly research aimed at prevention, increasing physical activity and attention to nutrition.


Wdowiak A.,Medical University of Lublin | Bakalczuk S.,Non Public Health Care Unit Ovum Reproduction and Andrology | Bakalczuk G.,Medical University of Lublin
Reproductive Biology | Year: 2015

The genetic integrity of sperm DNA can contribute to the infertility problems experienced by couples. Sperm DNA fragmentation (SDF) is the most common DNA abnormality in male gametes, and yet its effect on embryo kinetics has not been widely studied. Embryo morphokinetic parameters during the first days of embryo culture after intracytoplasmatic sperm injection (ICSI) are weakly predictive of both embryo development and pregnancy outcome. This study investigated the effect of SDF on embryo morphokinetic parameters following ICSI. The DNA fragmentation index (DFI) in spermatozoa from all males in the study (n=165) was determined before ICSI and the morphokinetic parameters of the embryos (n=165) were monitored via time-lapse recording. We found that a low DFI index in spermatozoa corresponded with embryos that reached the blastocyst stage at a faster rate after ICSI. Overall, lower SDF levels were also found in the group of patients that achieved pregnancy. Thus, higher SDF levels can slow down embryo morphokinetic parameters, and may be predictive of pregnancy outcomes after ICSI. © 2015 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn.


Wdowiak A.,Medical University of Lublin | Lewicka M.,Medical University of Lublin | Plewka K.,Non Public Health Care Unit Ovum Reproduction and Andrology | Bakalczuk G.,Medical University of Lublin
Annals of Agricultural and Environmental Medicine | Year: 2013

Introduction: According to the World Health Organization, infertility is defined as the inability to conceive following 12 months of regular unprotected sexual intercourse. Cigarette smoking, alcohol and drugs are the main stimulants exerting a negative effect on the male and female reproductive organs. Objective: The objective of the study was analysis of the effect of cigarette smoking by the women examined and their partners on the quality of embryos obtained in in vitro fertilization programmes. Material and methods: The study covered 54 women treated due to infertility. The database and statistical analyses were performed by means of computer software STATISTICA 7.1 (StatSoft, Poland). Results: The study showed that among 100% of the women examined, 24.07% smoked cigarettes. No statistically significant difference was observed between cigarette smoking by the women in the study (p=0.42), and the number of cigarettes smoked daily (p=0.52) and the total duration of smoking expressed in years (p=0.56). In addition, the study showed that 33.33% of respondents were exposed to passive nicotinism, while 66.67% were not exposed to passive smoking. In the group of women exposed to passive smoking, Class A embryos constituted 11.11%, Class B embryos - 83.38%, whereas Class C embryos - only 5.56%. A statistically significant relationship was noted between classes of embryos and exposure to passive nicotinism (p=0.03). Passive smoking results in the development of embryos of poorer quality. A significantly higher number of Class 2 embryos were produced from oocytes of women exposed to the effect of cigarette smoke, compared to Class 1. Among women at reproductive age, an active campaign should be carried out against nicotinism on behalf of their fertility and future maternity.


Wdowiak A.,Medical University of Lublin | Bakalczuk G.,Medical University of Lublin | Bakalczuk S.,Non Public Health Care Unit Ovum Reproduction and Andrology
Postȩpy higieny i medycyny doświadczalnej (Online) | Year: 2015

INTRODUCTION: Lead and cadmium can lead to negative effects on sperm chromatin DNA integrity. Copper, zinc and selenium are essential components of many enzymes which are important for reproduction. The aim of this research was to evaluate the influence of lead, cadmium, zinc, copper and selenium on the dynamics of semen DNA fragmentation.MATERIAL AND METHODS: The present study concerned 85 fertile and 131 infertile men aged 25-35. DNA fragmentation in the samples was determined after 3 h, 6 h and 12 h. The Pb, Cd, Cu, Zn, and Se measurements were performed by the electrothermal-atomic absorption spectrometry method.RESULTS: We found that sperm DNA fragmentation was a dynamic process which was intensified with an increase in the level of lead in seminal plasma. The levels of lead and cadmium were higher in seminal plasma of infertile men, compared to fertile men. The levels of zinc, copper and selenium in seminal plasma were higher in men with proven fertility, compared to infertile men, and did not exert a significant effect on the dynamics of sperm DNA fragmentation. The level of cadmium had no significant effect on intensification of sperm DNA fragmentation in time.DISCUSSION: Reports in the literature which concern the effect of trace elements on human reproduction are equivocal. The present study confirmed an unfavourable effect, especially that of lead, on the dynamics of sperm DNA fragmentation; however, these studies need to be expanded and continued in the future.


Wdowiak A.,Medical University of Lublin | Bakalczuk S.,Non Public Health Care Unit Ovum Reproduction and Andrology | Bakalczuk G.,Medical University of Lublin
Andrology | Year: 2015

Sperm DNA fragmentation varies between individuals and is more pronounced with increased patient age and time after sperm donation. The intensification of DNA fragmentation depends on the balance of the oxidoreductive system, which is regulated mainly by two enzymes - superoxide dismutase (SOD) and catalase. The objective of this study was to determine the relationship between sperm DNA fragmentation dynamics, fertility and seminal SOD and catalase activity. The study was conducted in 2013 and 2014 at the Non-Public Health Care Unit 'Ovum Reproduction and Andrology' in Lublin, Lublin, Poland, and covered 218 men aged 25-35 (85 fertile and 133 patients treated for infertility). Percentage of fragmented DNA was measured in a modified chromatin dispersion test at four time points after sperm donation (t = 0, 3, 6, 12 h). SOD and catalase activities were determined spectrophotometrically. We confirmed that the activity of SOD in the seminal plasma of men with reproductive disorders was lower compared with fertile men. Conversely, no significant correlations were found between fertility and catalase activity. Sperm DNA of infertile males was initially more fragmented than fertile male sperm DNA. SOD and catalase activity did not correlate with the degree of DNA fragmentation in fertile men. In men with reproductive disorders, the rate of DNA fragmentation was slow within first 3 h after sperm donation and then increased between 6 and 12 h. In this group of infertile men, those with higher SOD activity had a lower DNA fragmentation index (DFI) after 12 h, and a reduced rate of intensity of fragmentation from 6 to 12 h. Alternatively, higher catalase activity among men treated for infertility was accompanied by higher initial DFI and higher rate of DNA fragmentation from 6 to 12 h. These results highlight the importance of determining a proper time window between sperm donation and procedures of assisted reproductive technology. © 2015 American Society of Andrology and European Academy of Andrology.


PubMed | Medical University of Lublin and Non Public Health Care Unit Ovum Reproduction and Andrology
Type: | Journal: Postepy higieny i medycyny doswiadczalnej (Online) | Year: 2016

Lead and cadmium can lead to negative effects on sperm chromatin DNA integrity. Copper, zinc and selenium are essential components of many enzymes which are important for reproduction. The aim of this research was to evaluate the influence of lead, cadmium, zinc, copper and selenium on the dynamics of semen DNA fragmentation.The present study concerned 85 fertile and 131 infertile men aged 25-35. DNA fragmentation in the samples was determined after 3 h, 6 h and 12 h. The Pb, Cd, Cu, Zn, and Se measurements were performed by the electrothermal-atomic absorption spectrometry method.We found that sperm DNA fragmentation was a dynamic process which was intensified with an increase in the level of lead in seminal plasma. The levels of lead and cadmium were higher in seminal plasma of infertile men, compared to fertile men. The levels of zinc, copper and selenium in seminal plasma were higher in men with proven fertility, compared to infertile men, and did not exert a significant effect on the dynamics of sperm DNA fragmentation. The level of cadmium had no significant effect on intensification of sperm DNA fragmentation in time.Reports in the literature which concern the effect of trace elements on human reproduction are equivocal. The present study confirmed an unfavourable effect, especially that of lead, on the dynamics of sperm DNA fragmentation; however, these studies need to be expanded and continued in the future.


PubMed | Medical University of Lublin and Non Public Health Care Unit Ovum Reproduction and Andrology
Type: Journal Article | Journal: Reproductive biology | Year: 2015

The genetic integrity of sperm DNA can contribute to the infertility problems experienced by couples. Sperm DNA fragmentation (SDF) is the most common DNA abnormality in male gametes, and yet its effect on embryo kinetics has not been widely studied. Embryo morphokinetic parameters during the first days of embryo culture after intracytoplasmatic sperm injection (ICSI) are weakly predictive of both embryo development and pregnancy outcome. This study investigated the effect of SDF on embryo morphokinetic parameters following ICSI. The DNA fragmentation index (DFI) in spermatozoa from all males in the study (n = 165) was determined before ICSI and the morphokinetic parameters of the embryos (n = 165) were monitored via time-lapse recording. We found that a low DFI index in spermatozoa corresponded with embryos that reached the blastocyst stage at a faster rate after ICSI. Overall, lower SDF levels were also found in the group of patients that achieved pregnancy. Thus, higher SDF levels can slow down embryo morphokinetic parameters, and may be predictive of pregnancy outcomes after ICSI.


PubMed | Medical University of Lublin and Non Public Health Care Unit Ovum Reproduction and Andrology
Type: Journal Article | Journal: Przeglad menopauzalny = Menopause review | Year: 2016

To evaluate whether the hypothesis that estrogen levels are associated with temporomandibular disorders (TMD) in humans can be confirmed or contradicted by available literature.A systematic review based on the content of PubMed, Scopus, and Cochrane Library databases was performed. Studies were identified using a combination of key words temporomandibular disorder and estrogen. Nine studies were included into our review.The relationship between estrogen levels and TMD was found in seven out of nine reviewed papers. Results from two papers suggest that a high estrogen level is associated with an increased prevalence of TMD. Five additional papers found a relationship between a low estrogen level and an increase in TMD pain. In considering the value of evidence and inconsistencies of results in the reviewed publications, we state that there is weak evidence to support the hypothesis that estrogen levels are associated with TMD.Results of reviewed studies were divergent and sometimes contradictory. One possible explanation is that estrogen influences TMD pain processing differently than temporomandibular joints (TMJ) structures, as shown in many animal studies. Estrogen may influence TMD pain processing differently than TMJ structures. We suggest consideration of the dual action of estrogen when planning future studies on its association with TMD.

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