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Portacomaro, Italy

Sverzut A.T.,University of Sao Paulo | Crippa G.E.,University of Sao Paulo | Morra M.,Nobil Bio Ricerche Srl | De Oliveira P.T.,University of Sao Paulo | And 2 more authors.
Biomedical Materials | Year: 2012

The investigation of titanium (Ti) surface modifications aiming to increase implant osseointegration is one of the most active research areas in dental implantology. This study was carried out to evaluate the benefits of coating Ti with type I collagen on the osseointegration of dental implants. Acid etched Ti implants (AETi), either untreated or coated with type I collagen (ColTi), were placed in dog mandibles for three and eight weeks for histomorphometric, cellular and molecular evaluations of bone tissue response. While the histological aspects were essentially the same with both implants being surrounded by lamellar bone trabeculae, histomorphometric analysis showed more abundant bone formation in ColTi, mainly at three weeks. Cellular evaluation showed that cells harvested from bone fragments in close contact with ColTi display lower proliferative capacity and higher alkaline phosphatase activity, phenotypic features associated with more differentiated osteoblasts. Confirming these findings, molecular analyses showed that ColTi implants up-regulates the expression of a panel of genes well known as osteoblast markers. Our results present a set of evidences that coating AETi with collagen fastens the osseointegration by stimulating bone formation at the cellular and molecular levels, making this combination of morphological and biochemical modification a promising approach to treat Ti surfaces. © 2012 IOP Publishing Ltd.

Ferraris S.,Polytechnic University of Turin | Pan G.,Polytechnic University of Turin | Cassinelli C.,Nobil Bio Ricerche Srl | Mazzucco L.,SIMT ASO SS Antonio e Biagio | And 2 more authors.
Biomedical Materials (Bristol) | Year: 2012

Grafting of the biomaterial surfaces with biomolecules is nowadays a challenging research field for prosthetic and bone tissue engineering applications. On the other hand, very few research works investigate the effect of the sterilization processes on the properties of functionalized biomaterials. In this study, the effects of different sterilization techniques (e.g. gamma and electron beam irradiation, ethylene oxide) on the enzymatic activity of bioactive glasses and Ti6Al4V grafted with alkaline phosphatase (ALP) have been analyzed. Sterility maintenance and in vitro bioactivity of the sterilized surfaces have also been investigated. Finally the effect of packaging and storage conditions has been considered. © 2012 IOP Publishing Ltd.

NOBIL BIO RICERCHE S.r.l. | Date: 2011-03-24

Implantable devices having antibacterial activity for preventing periprosthetic infections and for improving osteointegration capacity are provided. Methods for making and using such devices are also provided.

Morra M.,Nobil Bio Ricerche Srl | Giavaresi G.,Rizzoli Orthopaedic Institute | Sartori M.,Rizzoli Orthopaedic Institute | Ferrari A.,Rizzoli Orthopaedic Institute | And 5 more authors.
Journal of Materials Science: Materials in Medicine | Year: 2015

The paper presents results of physico-chemical and biological investigations of a surface-engineered synthetic bone filler. Surface analysis confirms that the ceramic phosphate granules present a collagen nanolayer to the surrounding environment. Cell cultures tests show that, in agreement with literature reports, surface-immobilized collagen molecular cues can stimulate progression along the osteogenic pathway of undifferentiated human mesenchymal cells. Finally, in vivo test in a rabbit model of critical bone defects shows statistically significant increase of bone volume and mineral apposition rate between the biomimetic bone filler and collagen-free control. All together, obtained data confirm that biomolecular surface engineering can upgrade the properties of implant device, by promoting more specific and targeted implant-host cells interactions. © 2015, The Author(s).

Bollati D.,Nobil Bio Ricerche Srl | Morra M.,Nobil Bio Ricerche Srl | Cassinelli C.,Nobil Bio Ricerche Srl | Lupi S.M.,University of Pavia | Ruggero Rodriguez Y.B.,University of Pavia
BioMed Research International | Year: 2016

The goal of the present work was to investigate the relationship between in vivo healing and inflammatory response and in vitro cytokine expression by macrophages of a synthetic bone filler (25% hydroxylapatite-75% β-tricalcium phosphate) bearing a surface nanolayer of collagen. A clinically accepted, state-of-the-art xenograft material was used as a "negative control," that is, as a material that provides the correct clinical response for the intended use. In vitro data show that both materials exert a very low stimulation of proinflammatory cytokines by macrophages, and this was confirmed by the very mild inflammatory response detected in in vivo tests of local response in a rabbit model. Also, in vitro findings suggest a different mechanism of healing for the test and the control material, with a higher regenerative activity for the synthetic, resorbable filler, as confirmed by in vivo observation and literature reports. Thus, the simple in vitro model adopted provides a reasonable forecast of in vivo results, suggesting that new product development can be guided by in vitro tuning of cell-materials interactions. © 2016 Daniele Bollati et al.

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