Gunther M.,Nobelstr. |
Grumaz C.,Nobelstr. |
Lorenz S.,Nobelstr. |
Stevens P.,University of Stuttgart |
And 8 more authors.
Applied Microbiology and Biotechnology | Year: 2015
The basidiomycetous fungus Pseudozyma aphidis is able to convert vegetable oils to abundant amounts of the biosurfactant mannosylerythritol lipid (MEL) with a unique product pattern of MEL-A, MEL-B, MEL-C, and MEL-D. To investigate the metabolism of MEL production, we analyzed the transcriptome of P. aphidis DSM 70725 under MEL-inducing and non-inducing conditions using deep sequencing. Following manual curation of the previously described in silico gene models based on RNA-Seq data, we were able to generate an experimentally verified gene annotation containing 6347 genes. Using this database, our expression analysis revealed that only four of the five cluster genes required for MEL synthesis were clearly induced by the presence of soybean oil. The acetyltransferase encoding gene PaGMAT1 was expressed on a much lower level, which may explain the secretion of MEL with different degrees of acetylation in P. aphidis. In parallel to MEL synthesis, microscopic observations showed morphological changes accompanied by expression of genes responsible for cell development, indicative of a coregulation between MEL synthesis and cell morphology. In addition a set of transcription factors was identified which may be responsible for regulation of MEL synthesis and cell development. The upregulation of genes required for nitrogen metabolism and other assimilation processes indicate additional metabolic pathways required under the MEL-inducing conditions used. We also searched for a conserved gene cluster for cellobiose lipids (CL) but only found seven genes with limited homology distributed over the genome. However, we detected characteristic TLC spots in fermentations using P. aphidis DSM 70725, indicative of CL secretion. © 2015, Springer-Verlag Berlin Heidelberg.
News Article | October 29, 2016
InkRouter, a subsidiary of Opensoft Inc., creates solutions to workflow challenges in the printing industry. On Nov. 17, Opensoft Inc. CEO Brett Heap will be presenting the software’s JDF application at the CIP4 Workflow Symposium on JDF, Industry 4.0 and Workflow Automation, a global organization and symposium for printers and vendors taking place in Stuttgart, Germany. Scheduled to discuss the future role of technology in print in “Job Routing and Product Designing/Visualizing,” Heap will also present several product offerings from Opensoft that utilize JDF to ease cross-vendor workflow applications in pre-press. “At Opensoft, we’re all about creating software solutions to the issues of the print industry. How can we make printing more efficient and profitable?” said Heap. “Getting to share the InkRouter story with students is very exciting to me. They are the next generation of our work, and will be benefiting from and creating the next big software solution.” InkRouter is a print procurement solution that facilitates print orders between print resellers and print providers. The software for web-to-print storefronts uses a bi-directional API technology based on JDF/CIP4 compatible XML definitions to automate the process. Through the software, print brokers and web-to-print storefronts set specific criteria for job requests. InkRouter intelligently selects a print provider that matches the given price limits and turnaround times. As members of the InkRouter network, print providers can expand sales forces beyond a local network to bid on jobs globally. To be held at Hochschule der Medien, Nobelstr. 10, 70569 Stuttgart, Hall 011-013, the 8th CIP4 Workflow Symposium will host presentations and live product demonstrations for printers, publishers, media experts and vendors. The “Job Routing and Product Designing/Visualizing” presentation is scheduled for 1:30 p.m. For more information on InkRouter, visit inkrouter.com About InkRouterSM InkRouter, Inc. is a wholly owned subsidiary of Opensoft Inc., located in Las Vegas, Nev. Opensoft Inc. has advanced technologies for the print industry and extensive experience in developing print software for managing print processes at every stage of selling, manufacturing and fulfillment.