Chongqing, China
Chongqing, China

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Guo L.,Chongqing Medical University | Li S.-Y.,Kunming General Hospital of Chengdu Military Command | Ji F.-Y.,Chongqing Medical University | Zhao Y.-F.,Pudong New Area Gongli Hospital | And 4 more authors.
Inflammation Research | Year: 2014

Background: Angptl4 is a secreted protein involved in the regulation of vascular permeability, angiogenesis, and inflammatory responses in different kinds of tissues. Increases of vascular permeability and abnormality changes in angiogenesis contribute to the pathogenesis of tumor metastasis, ischemic-reperfusion injury. Inflammatory response associated with Angptl4 also leads to minimal change glomerulonephritis, wound healing. However, the role of Angptl4 in vascular permeability, angiogenesis, and inflammation is controversy. Hence, an underlying mechanism of Angptl4 in different kind of tissues needs to be further clarified. Methods: Keywords such as angptl4, vascular permeability, angiogenesis, inflammation, and endothelial cells were used in search tool of PUBMED, and then the literatures associated with Angptl4 were founded and read. Results: Data have established Angptl4 as the key modulator of both vascular permeability and angiogenesis; furthermore, it may also be related to the progression of metastatic tumors, cardiovascular events, and inflammatory diseases. This view focuses on the recent advances in our understanding of the role of Angptl4 in vascular permeability, angiogenesis, inflammatory signaling and the link between Angptl4 and multiple diseases such as cancer, cardiovascular diseases, diabetic retinopathy, and kidney diseases. Conclusions: Taken together, Angptl4 modulates vascular permeability, angiogenesis, inflammatory signaling, and associated diseases. The use of Angptl4-modulating agents such as certain drugs, food constituents (such as fatty acids), nuclear factor (such as PPARα), and bacteria may treat associated diseases such as tumor metastasis, ischemic-reperfusion injury, inflammation, and chronic low-grade inflammation. However, the diverse physiological functions of Angptl4 in different tissues can lead to potentially deleterious side effects when used as a therapeutic target. In this regard, a better understanding of the underlying mechanisms for Angptl4 in different tissues is necessary. © 2013 Springer Basel.


Zhong Y.,No324 Hospital Of Pla | Chen Z.,General Hospital of Chengdu Military Region of PLA | Chen L.-B.,No324 Hospital Of Pla | Liao M.-S.,General Hospital of Chengdu Military Region of PLA | Deng D.,General Hospital of Chengdu Military Region of PLA
Chinese Journal of Medical Imaging Technology | Year: 2013

Objective: To investigate the effect of high mechanical index diagnostic ultrasound combined with microbubbles on the neovasculature of Walker-256 tumor in rats. Methods: Twenty-nine SD rats bearing subcutaneous Walker-256 tumor were randomly divided into 3 groups: Ultrasound+microbubbles group (n=15), ultrasound group (n=7) and the sham group (n=7). CEUS was performed to acquire the baseline tumor blood perfusion of all the tumors. During the procedure, the tumors were insonated with 20 times of continuous acoustic radiation force impulse imaging. At the same time of ARFI performance, 0.04 ml microbubbles was instantly injected into the tail vein. The ultrasound group and sham group were applied with only diagnostic ultrasound exposure or microbubbles injection, respectively. CEUS was repeated to observe the changes of perfusion before insonation and 0, 10 and 20 min after insonation. Three rabbits were harvested randomly in every group for histological examination immediately after insonation. Results: The mean peak intensity (PI) values of ROI displayed significant decreases from 25.17% to 12.01% in ultrasound+microbubbles group (P<0.01). In the ultrasound group and sham group, media was filling quickly and perfusion was good. In ultrasound+microbubbles group, the blood flow recovered gradually, but PI still reduced after 10 min, the blood flow of tumor recovered basically complete and showed high perfusion phase after 20 min (P>0.05). Conclusion: Microbubbles enhanced high mechanical index diagnostic ultrasound can temporally decrease the microcirculation of Walker-256 tumor in rats. Copyright © 2013 by the Press of Chinese Journal of Medical Imaging Technology.


Hao L.,No324 Hospital Of Pla | Zou Z.,Beijing Research Institute of Chemical Defense | Tian H.,No324 Hospital Of Pla | Zhang Y.,No324 Hospital Of Pla | And 2 more authors.
BioMed Research International | Year: 2014

In recent years, stem cell-based approaches have attracted more attention from scientists and clinicians due to their possible therapeutical effect on stroke. Animal studies have demonstrated that the beneficial effects of stem cells including embryonic stem cells (ESCs), inducible pluripotent stem cells (iPSCs), neural stem cells (NSCs), and mesenchymal stem cell (MSCs) might be due to cell replacement, neuroprotection, endogenous neurogenesis, angiogenesis, and modulation on inflammation and immune response. Although several clinical studies have shown the high efficiency and safety of stem cell in stroke management, mainly MSCs, some issues regarding to cell homing, survival, tracking, safety, and optimal cell transplantation protocol, such as cell dose and time window, should be addressed. Undoubtably, stem cell-based gene therapy represents a novel potential therapeutic strategy for stroke in future. © 2014 Lei Hao et al.


Tu W.-B.,East China Jiaotong University | Luo Y.,East China Jiaotong University | Lei T.,No324 Hospital Of Pla
Dandao Xuebao/Journal of Ballistics | Year: 2016

To overcome the problem that large deformation and splash in high speed impact process can't be simulated well by the traditional finite element method(FEM),a simulation method of the broken bone flying,namely node separation-coupling method,was proposed aiming at the process of bullets penetrating mandible.The FEM model of the bullet penetrating pig mandible was built by using hexahedron element,and the numerical simulation of the high-speed bullet penetrating mandible process was carried out,and the failure and separation of the elements were realized by defining the failure strain value of the material and the nodes failure constraint.The splashing form of the pig mandible,the distribution and the speed of the flying elements during the splashing process were analyzed.The results show that the simulation results are in good agreement with the experimental data,and the splashing process of the mandible can be well simulated by this method,and it provides a new idea and method for the study of biomechanical properties and damage mechanism of bone damage. © 2016, Editorial Board of Journal of Ballistics. All right reserved.


Cai Y.,No324 Hospital Of Pla
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology | Year: 2010

To identify the HBV genotype-specific tag sequence. The large S region sequences from 930 HBV genomes were aligned to identify the genotype-specific tag sequences. PCR was used to check the genotyping effect of these tags. Two tag sequences, sequence between 149-169 and sequence between 461-483, were identified in the large S region. Using primers specific to these tag sequences, the genotype of HBV can be specifically identified. These tag sequences can be used for HBV genotyping.


Cai Y.,No324 Hospital Of Pla | Wu X.,No324 Hospital Of Pla | Shi Y.,No324 Hospital Of Pla | Liu W.,No324 Hospital Of Pla
Open Life Sciences | Year: 2015

The aim of this work was to investigate the different evolutionary patterns between HBeAg(+) and HBeAg(-) status of genotype B and genotype C of Hepatitis B virus (HBV). Complete HBV genome sequences were obtained from GenBank, and were classified as HBeAg(+) of genotype B, HBeAg(-) of genotype B, HBeAg(+) of genotype C, and HBeAg(-) of genotype C. The results showed that genotype B had lower diversity and a longer phylogenetic tree than genotype C. This means that genotype B was more conserved than genotype C. The genetic distance of HBeAg(+) was shorter than that of HBeAg(-) in genotype B. dN, dS, dN/dS of HBeAg(+) were lower than that of HBeAg(-). In addition, the Core and S genes had lower diversity, and were under lower selective pressure than other genes. We propose that by having different HBeAg HBV receives disparate immune selective pressures, however these different genotypes also receive disparate natural selective pressures. The evolutionary pattern of HBV could be one of the important factors that may affect viral replication in HBV infected patients and could therefore play an important role in the diseases progression. © 2015 Ying Cai et al. 2015.


Mao M.,General Hospital of Guangzhou Military Command | Mao M.,No324 Hospital Of Pla | Zhang B.,General Hospital of Guangzhou Military Command | Fu Z.-H.,General Hospital of Guangzhou Military Command | And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Endothelial progenitor cells play an important role in maintaining normal endothelial function as well as endothelial repairing after vascular injury, which has been widely applied to multiple clinical disorders including cardiovascular disease, limb ischemia and vascular repair. But the application studies of endothelial progenitor cells are much fewer in inflammatory diseases and lung injury. Objective: To observe the effect of endothelial progenitor cell transplantation on expressions of tumor necrosis factor-α and interleukin-10 in acute lung injury/acute respiratory distress syndrome rats and to explore whether cell transplantation can improve the inflammatory state of acute lung injury/acute respiratory distress syndrome. Methods: Thirty Sprague Dawley rats of the same genetic background were randomly divided into three groups with 10 rats in each group: normal control group, lung injury group and cell transplantation group. The endothelial progenitor cells were isolated and cultured with density gradient centrifugation method. Rats in the lung injury group and cell transplantation group were injected with lipopolysaccharide through tail vein to make the acute lung injury models. Rats in normal control group only received phosphate buffered solution with the same dose. Half an hour after modeling, rats in the normal control group and cell transplantation group were injected with bone marrow-derived endothelial progenitor cells suspension through tail vein, and rats in lung injury group received the same amounts of phosphate buffered solution. Results and Conclusion: Compared with the lung injury group, the expression of interleukin-10 was significantly increased in the cell transplantation group (P < 0.001), but the expression of tumour necrosis factor-α was reduced, and the difference was not significant (P > 0.05). It indicated that transplantation of endothelial progenitor cells could up-regulate interleukin-10 expression and down-regulate tumour necrosis factor-α expression, and could significantly improve the inflammatory state in injury lung tissues.


Tian H.,No324 Hospital Of Pla | Song C.,No324 Hospital Of Pla | Fan R.-X.,No324 Hospital Of Pla | Zhou H.-C.,No324 Hospital Of Pla | And 4 more authors.
Journal of Interventional Radiology | Year: 2011

Objective: To investigate the therapeutic method of cerebral ischemia-reperfusion injuries occurred after arterial thrombolytic therapy for acute cerebral infarction. Methods: Thirty-five patients, encountered in authors' Department since Oct. 2005, with cerebral ischemia-reperfusion injuries, which occurred after thrombolytic therapy by using arterial perfusion of urokinase for acute cerebral infarction, were enrolled in this study. The clinical data were retrospectively analyzed. Results: After the thrombolytic therapy, complete or partial recanalization of the occluded cerebral arteries was obtained in 33 cases, while secondary cerebral hemorrhage occurred in 13 cases, of whom cerebral parenchyma bleeding was seen in 2 and hemorrhagic infarction in 11. Different degrees of cerebral edema were found in all 33 cases. Among them significant shift of the midline structures was detected in 18 (54.5%), which was manifested clinically as the worsening of disturbance of consciousness. Strict control of blood pressure, prompt adjustment of dehydration medication, strengthening the cerebral protection measures, cerebral decompression by fenestration, etc. were carried out. All the patients took a turn for the better and were out of danger with remarkable improvement of neurological functions except one patient who died from massive intracerebral hemorrhage. Conclusion: Usually, different degrees of reperfusion injuries will develop after thrombolytic therapy for cerebral arterial infarction. Strictly controlling blood pressure, promptly adjusting dehydration medication and strengthening cerebral protection are the keys to reduce the severity of cerebral reperfusion injuries.


Zou C.,No324 Hospital Of Pla | Hao L.,No324 Hospital Of Pla | Tian H.,No324 Hospital Of Pla | Song C.,No324 Hospital Of Pla | And 3 more authors.
American Journal of the Medical Sciences | Year: 2016

Objective To explore the effect of perivascular sympathetic nerve on cerebral collateral arteriogenesis in chronic cerebral hypoperfusion models of rats. Materials and Methods Chronic cerebral hypoperfusion model was established by right common carotid artery ligation for 8 weeks, while sympathetic denervation was performed by superior cervical ganglionectomy. The male Sprague-Dawley rats were randomly divided into 4 groups including sham group (n = 21), denervation group (n = 21), artery ligation group (n = 21) and combined group with both artery ligation and denervation (n = 21). After 8 weeks of surgery, the rats in each group were randomly divided into 3 subgroups including subgroup A (n = 7), subgroup B (n = 7) and subgroup C (n = 7). The 3 subgroups were subjected to latex perfusion, permanent right middle cerebral artery occlusion and immunohistochemical staining, respectively. Results The diameters of right leptomeningeal anastomoses in artery ligation group significantly enlarged compared with sham group. When sympathetic denervation was performed in the presence of artery ligation, diameter of collateral vessel decreased, although larger than in sham group. After 8 weeks of permanent right middle cerebral artery occlusion, the cerebral perfusion over the right middle cerebral artery area in combined group was significantly lower than in artery ligation group, although both were higher than in denervation group and sham group. Triphenyltetrazolium chloride staining showed that cerebral infarct volume in combined group was significantly larger than in artery ligation group, and smaller than in denervation group and sham group. Neurologic functional scoring showed that scores in combined group were significantly higher than in artery ligation group, and lower than in denervation group and sham group. Immunohistochemical staining for α-smooth muscle actin showed that compared with sham group, tunica media thickness of right leptomeningeal anastomoses in artery ligation group increased significantly. Thickness in combined group was thinner than in artery ligation group, although thicker than in sham group. Conclusions Perivascular sympathetic denervation can impair the cerebral collateral arteriogenesis under condition of chronic cerebral hypoperfusion. © 2016 Southern Society for Clinical Investigation


PubMed | No324 Hospital Of Pla
Type: Journal Article | Journal: The American journal of the medical sciences | Year: 2016

To explore the effect of perivascular sympathetic nerve on cerebral collateral arteriogenesis in chronic cerebral hypoperfusion models of rats.Chronic cerebral hypoperfusion model was established by right common carotid artery ligation for 8 weeks, while sympathetic denervation was performed by superior cervical ganglionectomy. The male Sprague-Dawley rats were randomly divided into 4 groups including sham group (n = 21), denervation group (n = 21), artery ligation group (n = 21) and combined group with both artery ligation and denervation (n = 21). After 8 weeks of surgery, the rats in each group were randomly divided into 3 subgroups including subgroup A (n = 7), subgroup B (n = 7) and subgroup C (n = 7). The 3 subgroups were subjected to latex perfusion, permanent right middle cerebral artery occlusion and immunohistochemical staining, respectively.The diameters of right leptomeningeal anastomoses in artery ligation group significantly enlarged compared with sham group. When sympathetic denervation was performed in the presence of artery ligation, diameter of collateral vessel decreased, although larger than in sham group. After 8 weeks of permanent right middle cerebral artery occlusion, the cerebral perfusion over the right middle cerebral artery area in combined group was significantly lower than in artery ligation group, although both were higher than in denervation group and sham group. Triphenyltetrazolium chloride staining showed that cerebral infarct volume in combined group was significantly larger than in artery ligation group, and smaller than in denervation group and sham group. Neurologic functional scoring showed that scores in combined group were significantly higher than in artery ligation group, and lower than in denervation group and sham group. Immunohistochemical staining for -smooth muscle actin showed that compared with sham group, tunica media thickness of right leptomeningeal anastomoses in artery ligation group increased significantly. Thickness in combined group was thinner than in artery ligation group, although thicker than in sham group.Perivascular sympathetic denervation can impair the cerebral collateral arteriogenesis under condition of chronic cerebral hypoperfusion.

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