Yang K.,No107 Hospital Of Pla |
Wang Y.-S.,No107 Hospital Of Pla |
Zhao T.-D.,Postgraduate College
Chinese Journal of Cancer Prevention and Treatment | Year: 2012
To investigate the effect of euphorbia fischeriana steud (LDE) on apoptosis and expression of Caspase-9 mRNA in Lewis lung carcinoma. Lewis cells were exposed with LDE of different concentration (0.2, 0.4 and 0.8 mg/mL) for 24 hours. Annexin-V/PI double label staining was applied to detect the change of apoptosis rate. Flow cytometry was applied to detect the change of cell cycle. The mRNA level of Caspase-9 was detected by Real-time PCR. By Annexin-V/PI double label staining to examine apoptosis of control and low, middle and high LDE dose group (0.2, 0.4 and 0.8 mg/mL), and the rate respectively was (0.331 ± 0.012)%, (8.27 ± 0.067)%, (28.19 ± 0.270)%, (32.96 ± 0.14)%. Flow cytometry showed that the G0/G1 phase rate of the control and low, middle and high LDE dose group were (50.3 ± 0.77) %, (44.2 ± 1.82)%, (34.1 ± 1.56)%, (25 ± 0.72)%. Real-time PCR showed that Caspase-9 mRNA expression level of the control and LDE group were 1.00 and 1.64. LDE can effectively induce apoptosis of Lewis cells through blocking the cell cycle at G0/G1 phase and increase the expression of Caspase-9 mRNA level.