Gu H.,No.88 Hospital of PLA |
Yang M.,No.88 Hospital of PLA |
Zhao X.,Taishan Medical University |
Zhao B.,Taishan Medical University |
And 2 more authors.
Journal of Surgical Research | Year: 2014
Background Rhabdomyolysis is a leading cause of acute kidney injury. The pathophysiological process involves oxidative stress and inflammation. Hydrogen-rich saline (HRS) is an antioxidant and anti-inflammatory. This study explored the protective effect of pretreatment with HRS on the development of glycerol-induced rhabdomyolysis acute kidney injury. Materials and methods Forty-eight rats were randomly divided into four equal groups. Group 1 served as the control, group 2 was given 50% glycerol (10 mL/kg, intramuscular), group 3 was given glycerol after 7 d pretreatment with high dose HRS (10 mL/kg/d, intraperitoneal), and group 4 was given glycerol after 7 d pretreatment with low dose HRS (5 mL/kg/d, intraperitoneal). Renal health was monitored by serum creatinine (Cr), urea, and histologic analysis; rhabdomyolysis was monitored by creatine kinase (CK) levels; and oxidative stress was monitored by kidney tissue reactive oxygen species (ROS), malondialdehyde, 8-hydroxydeoxyguanosine (8-OH-dG), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) levels. Inflammation was monitored by interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) evaluation. Results Glycerol administration resulted in an increase in the mean histologic damage score, serum Cr, urea and CK, kidney tissue ROS, malondialdehyde, 8-OH-dG, GSH-PX, IL-6, and TNF-α, and a decrease in kidney tissue superoxide dismutase activity. All these factors were significantly improved by both doses of HRS, but the mean histologic damage score, urea, Cr, CK, ROS, 8-OH-dG, GSH-PX, IL-6, and TNF-α for the high dose HRS treatment group were even lower. Conclusions Pretreatment by HRS ameliorated renal dysfunction in glycerol-induced rhabdomyolysis by inhibiting oxidative stress and the inflammatory response. © 2014 Elsevier Inc. All rights reserved. Source
Zhang W.,No.88 Hospital of PLA |
Zhou L.,No.88 Hospital of PLA |
Li N.-Y.,No.88 Hospital of PLA |
Li Y.-P.,No.88 Hospital of PLA |
And 2 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2015
OBJECTIVE: To explore the role of UHRF1 (ubiquitinlike with PHD and ring finger domain 1) in gastric cancer development, and evaluate its significance in predicting prognosis in gastric cancer tissurs. METHODS: We examined UHRF1 expression in 106 gastric carcinoma tissues and 72 adjacent non-tumor tissues by immunohistochemical method and evaluated the relationship between UHRF1 expression and clinicopathological factors as well as prognosis. RESULTS: Positive expression of UHRF1 was detectable in 84.0%(89/106) of gastric carcinoma tissues and there was a significant difference, compared with positive rate in 72 adjacent non-tumor tissues (48.6%, χ2=35.220, P<0.001). Expression of UHRF1 correlated with differentiation (P<0.05), TNM staging (P<0.01), lymph node metastases (P<0.05), and metastasis (P<0.05), but not with age (P=0.351) and gender (P=0.427). Both univariate and multivariate survival analysis showed patients with high level of UHRF1 expression had shorter overall survival (P<0.001). CONCLUTION: Our findings suggest that UHRF1 is strongly related with gastric cancer development and can be a predictor for gastric cancer prognosis. ©, 2015, The Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved. Source