Tao W.,No. 513 Hospital of People s Liberation Army |
Guoxi W.,No. 513 Hospital of People s Liberation Army |
Weishan L.,No. 513 Hospital of People s Liberation Army |
Fuping G.,No. 513 Hospital of People s Liberation Army |
And 3 more authors.
Chinese Journal of Cancer Biotherapy
Objective:To investigate the expression of ubiquitin-conjugating enzyme H10 (UbcHIO) protein in menin-giomas tissues of different pathological grades and its clinical significance. Methods: Forty-seven patients diagnosed as meningioma with integrity clinical and follow-up information during April 2002 to September 2009 were selected from Department of Neurosurgery, Changzheng Hospital and were graded according to the 2007 WHO standard. The expressions of UbcHIO and Ki-67 proteins in meningioma tissues of various pathological grades were examined by immunohistochemis-try. The correlation between the expressions of UbcHIO and Ki-67 protein was analyzed by using Spearman method. The correlation of UbcHIO expression with meningioma recurrence-free surivival was analyzed using the Kaplan-Meier method. Results:UbcH 10 protein was mainly expressed in the cytoplasm of meningioma cells. The expression of UbcHIO protein in meningioma tissues was significantly higher than that in normal brain tissues (5. 57 ± 1. 72 \% vs 0,P =0. 00). Quantitative analysis confirmed that the expression of UbcHIO protein was significantly higher in atypical and anaplastic meningi-omas as compared with classic meningiomas (10. 53 ±5. 79 \% vs [4. 23 ±2. 85 J%, P <0. 01), indicating that the expression of UbcHIO protein was correlated with meningioma pathological grade and recurrence. The expression of UbcHIO protein was correlated with the expression of Ki-67 protein (r-0. 77, P < 0. 01). Kaplan-Meier survival curve result indicated patients with high expression level of UbcHIO and Ki-67 and higher pathological grade showed a significantly shortened recurrence-free surivival than those with a lower expression level of UbcHIO (P-0. 007) and Ki-67 (P-0. 018), and a lower pathological grade (P <0. 01). Cox multivariate regression analysis showed that the pathological grade (P-0. 029) and the expression level of UbcHIO (P-0. 044) were independent prognosis factors for meningioma patients, with the hazard ratio of 2.918 and 4. 756, respectively. Whereas, the clinicopathological factors, including age, gender, Ki-67 level, tumor diameter and tumor location were not independent factors for prognosis. Conclusion: UbcHIO may be involved in the development and progression of meningioma, and UbcHIO expression is an independent risk factor that could predict the prognosis. Source