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Shanghai, China

Liu L.,Fujian University of Traditional Chinese Medicine | Liu L.,Shanghai University | Han T.,Shanghai University | Xue L.-M.,Shanghai University | And 5 more authors.
Academic Journal of Second Military Medical University | Year: 2010

Objective To optimize the extraction process for phenolic glycosides from Rhizoma Curculigins. Methods The contents of orcinol glucoside,curculigoside and curculigine were used as parameters, and they were determined by HPLC. The extraction conditions of phenolic glycosides from Rhizoma Curculigins were optimized using orthogonal design. The examined factors included concentration of ethanol(50% , 70% , and 95%) , extraction time (1, 1.5, and 2 h) , times of extraction (1,2, and 3) , and solvent volume (6, 8, and 10 times). Results The concentration of ethanol, extraction time, times of extraction and solvent volume significantly affected the extraction efficacy of phenolic glycosides in Rhizoma Curculigins, with the most prominent effect exerted by the ethanol concentration. The optimal extraction process was refluxing three times with 70% ethanol (10 times of solvent volume), for 2 h each time. Conclusion The present method can effectively extract phenolic glycosides from Rhizoma Curculigin, which paves a way for further research on phenolic glycosides in Rhizoma Curculigins.

Xue X.-C.,No. 101 Hospital of PLA | Liu F.,No. 455 Hospital of PLA
Academic Journal of Second Military Medical University | Year: 2011

Objective To observe the effect of ligustrazine hydrochioridc(LHC) injection on TGF-β1 -induced proliferation and type IV collagen secretion in the human mesangial cells(HMCs). Methods The interstitial fibrosis in kidney disease was mimicked by inducing proliferation and type IV collagen secretion in HMCs with TGF- β1. The experiment was divided into 5 groups: blank, control, low, medium, and high(10, 30, and 100 μg/ml)ligustrazinc hydrochloride groups. MTT method was adopted to examine the proliferation and inhibition rate of HMCs. Enzyme-linked immunosorbent assay was used to determine the production of type IV collagen in cultured HMCs. Results LHC at high concentration (100 μg/ml) significantly inhibited the proliferation of HMCs (P<0. 01). LHC also inhibited the production of type IV collagen, with the significant inhibition found when at the concentration of 100 μg/ml (P

Wang G.,Shanghai University | Dong L.,No. 455 Hospital of PLA | Zhao H.,Shanghai University | Zhao Y.,Shanghai University | Zhao Z.,Shanghai University
Chinese Journal of Cancer Biotherapy | Year: 2013

Objective: To investigate the effect of melatonin (MT) and cisplatin (DDP) used alone or in combination on proliferation and apoptosis of human glioma cells U251 and SHG-44. Methods: U251 and SHG-44 cells were treated with various concentrations of MT and DDP alone or in combination, and the control group (without adding any drug) and ethanol group (adding the ethanol) were included. CCK-8 assay was used to detect cell proliferation, and flow cytometry was used to detect cell apoptosis and cell cycle. The coefficient of drug interaction (CDI) was used to evaluate whether MT could affect the sensitivity of U251 and SHG-44 cells to DDP. Results: CCK-8 results showed that MT or DDP used alone inhibited the proliferation of U251 and SHG-44 cells in a concentration dependent manner, and 0.5 mmol/L MT synergistically enhanced the proliferation inhibitory effect of DDP on U251 and SHG-44 cells (CDI<1). Flow cytometry results showed that MT promoted U251 and SHG-44 cell apoptosis, and MT enhanced the apoptosis inducing effect of DDP on U251 and SHG-44 cells. The apoptotic rate of U251 and SHG-44 cells in the 0.5 mmol/L MT and 20 μg/ml DDP combination group was significantly higher than that in 20 μg/ml DDP group (\[66.3±1.0\]% vs \[45.9±1.7\]%, \[35.5±0.8\]% vs \[15.5±0.8\]%, P<0.01). And the percentage of U251 and SHG-44 cells in sub-G1 phase in the 0.5 mmol/L MT and 20 μg/ml DDP combination group was significantly higher than that in 20 μg/ml DDP group (\[52.4±2.1\]% vs \[27.9±1.5\]%, \[39.7±1.5\]% vs \[27.7±1.3\]%, P<0.01\]. Conclusion: MT can enhance the apoptosis inducing effect of DDP on human glioma cells U251 and SHG-44, thereby synergistically enhancing the suppressive effects of DDP on human glioma cell proliferation, which may be used as a complementary drug in human glioma chemotherapy.

Wu J.,No. 411 Hospital of PLA | Luo D.,No. 455 Hospital of PLA | Ye X.,Spine Surgery | Luo X.,No. 411 Hospital of PLA | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

To clarify anatomy-related risk factors in the cervical spine with subsidence of titanium mesh cage (TMC) after one-level cervical corpectomy and fusion, we have assessed the radiological examinations and clinical outcomes for 236 patients. All the patients were underwent one-level corpectomy and TMC fusion between August 2003 and March 2006. The effects of the cervical posture, segmental curvature and endplate gradient on the postoperative phenomenon for these patients were evaluated. Our results suggested that in the patients who were followed up for 12 months, TMC subsidence occurred in 54 (28.6%) cases. C6 corpectomy had a significant higher risk (26/60, 43.3%) for TMC subsidence, which was correlated with the variation of the gradient of the vertebral endplates against cervical levels. Although the clinical outcome was comparable with those in the literature, the patients may have subsidence-related problems such as neck-shoulder pain, neurological deterioration and instrumental failure. In conclusion, to reduce the incidence of subsidence, TMC design should be optimized to be in line with anatomic characteristics of the cervical spine. © 2015, E-Century Publishing Corporation. All rights reserved.

Bo H.,No. 455 Hospital of PLA | Gao L.,Shanghai University | Chen Y.,Shanghai University | Zhang J.,Shanghai University | Zhu M.,Shanghai University
Molecular Medicine Reports | Year: 2016

Wnt proteins are a group of secreted signaling proteins, which function to regulate cell fate and pattern formation during embryogenesis. Altered expression of Wnt5a has been implicated in human carcinogenesis and tumor progression. A previous study identified that Wnt5a is overexpressed in human pancreatic cancer tissues, and that upregulated expression of Wnt5a promotes tumor cell migration and invasion. The present study investigated the role of Wnt5a in pancreatic cancer cell proliferation in vitro and in an orthotopic nude mouse model. Wnt5a cDNA or small interfering RNA were stably transfected into pancreatic cancer cells to assess cell proliferation-associated behaviors, including cell viability, colony formation and apoptosis in vitro, as well as tumor cell growth in an orthotopic nude mouse model. Western blot analysis was used to analyze the expression of Wnt signaling molecules. The data showed that upregulation of the expression of Wnt5a significantly promoted proliferation of the human pancreatic cells, but inhibited tumor cell apoptosis in vitro and promoted tumor growth in an orthotopic nude mouse model. By contrast, knockdown of the expression of Wnt5a inhibited cell growth and promoted apoptosis of the pancreatic cancer cells. The data also revealed that β-catenin mediated the effects of Wnt5a on the regulation of pancreatic cancer cell apoptosis in vitro. These results suggested that Wnt5a is involved in the modulation of pancreatic cancer cell proliferation, and that Wnt5a may be a potential target for pancreatic cancer therapy.

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