No 455 Hospital Of Pla

Shanghai, China

No 455 Hospital Of Pla

Shanghai, China
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Wang G.,Shanghai University | Dong L.,No 455 Hospital Of Pla | Zhao H.,Shanghai University | Zhao Y.,Shanghai University | Zhao Z.,Shanghai University
Chinese Journal of Cancer Biotherapy | Year: 2013

Objective: To investigate the effect of melatonin (MT) and cisplatin (DDP) used alone or in combination on proliferation and apoptosis of human glioma cells U251 and SHG-44. Methods: U251 and SHG-44 cells were treated with various concentrations of MT and DDP alone or in combination, and the control group (without adding any drug) and ethanol group (adding the ethanol) were included. CCK-8 assay was used to detect cell proliferation, and flow cytometry was used to detect cell apoptosis and cell cycle. The coefficient of drug interaction (CDI) was used to evaluate whether MT could affect the sensitivity of U251 and SHG-44 cells to DDP. Results: CCK-8 results showed that MT or DDP used alone inhibited the proliferation of U251 and SHG-44 cells in a concentration dependent manner, and 0.5 mmol/L MT synergistically enhanced the proliferation inhibitory effect of DDP on U251 and SHG-44 cells (CDI<1). Flow cytometry results showed that MT promoted U251 and SHG-44 cell apoptosis, and MT enhanced the apoptosis inducing effect of DDP on U251 and SHG-44 cells. The apoptotic rate of U251 and SHG-44 cells in the 0.5 mmol/L MT and 20 μg/ml DDP combination group was significantly higher than that in 20 μg/ml DDP group (\[66.3±1.0\]% vs \[45.9±1.7\]%, \[35.5±0.8\]% vs \[15.5±0.8\]%, P<0.01). And the percentage of U251 and SHG-44 cells in sub-G1 phase in the 0.5 mmol/L MT and 20 μg/ml DDP combination group was significantly higher than that in 20 μg/ml DDP group (\[52.4±2.1\]% vs \[27.9±1.5\]%, \[39.7±1.5\]% vs \[27.7±1.3\]%, P<0.01\]. Conclusion: MT can enhance the apoptosis inducing effect of DDP on human glioma cells U251 and SHG-44, thereby synergistically enhancing the suppressive effects of DDP on human glioma cell proliferation, which may be used as a complementary drug in human glioma chemotherapy.


Wu J.,No 411 Hospital Of Pla | Luo D.,No 455 Hospital Of Pla | Ye X.,Shanghai Changzheng Hospital | Luo X.,No 411 Hospital Of Pla | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

To clarify anatomy-related risk factors in the cervical spine with subsidence of titanium mesh cage (TMC) after one-level cervical corpectomy and fusion, we have assessed the radiological examinations and clinical outcomes for 236 patients. All the patients were underwent one-level corpectomy and TMC fusion between August 2003 and March 2006. The effects of the cervical posture, segmental curvature and endplate gradient on the postoperative phenomenon for these patients were evaluated. Our results suggested that in the patients who were followed up for 12 months, TMC subsidence occurred in 54 (28.6%) cases. C6 corpectomy had a significant higher risk (26/60, 43.3%) for TMC subsidence, which was correlated with the variation of the gradient of the vertebral endplates against cervical levels. Although the clinical outcome was comparable with those in the literature, the patients may have subsidence-related problems such as neck-shoulder pain, neurological deterioration and instrumental failure. In conclusion, to reduce the incidence of subsidence, TMC design should be optimized to be in line with anatomic characteristics of the cervical spine. © 2015, E-Century Publishing Corporation. All rights reserved.


Bo H.,No 455 Hospital Of Pla | Gao L.,Shanghai University | Chen Y.,Shanghai University | Zhang J.,Shanghai University | Zhu M.,Shanghai University
Molecular Medicine Reports | Year: 2016

Wnt proteins are a group of secreted signaling proteins, which function to regulate cell fate and pattern formation during embryogenesis. Altered expression of Wnt5a has been implicated in human carcinogenesis and tumor progression. A previous study identified that Wnt5a is overexpressed in human pancreatic cancer tissues, and that upregulated expression of Wnt5a promotes tumor cell migration and invasion. The present study investigated the role of Wnt5a in pancreatic cancer cell proliferation in vitro and in an orthotopic nude mouse model. Wnt5a cDNA or small interfering RNA were stably transfected into pancreatic cancer cells to assess cell proliferation-associated behaviors, including cell viability, colony formation and apoptosis in vitro, as well as tumor cell growth in an orthotopic nude mouse model. Western blot analysis was used to analyze the expression of Wnt signaling molecules. The data showed that upregulation of the expression of Wnt5a significantly promoted proliferation of the human pancreatic cells, but inhibited tumor cell apoptosis in vitro and promoted tumor growth in an orthotopic nude mouse model. By contrast, knockdown of the expression of Wnt5a inhibited cell growth and promoted apoptosis of the pancreatic cancer cells. The data also revealed that β-catenin mediated the effects of Wnt5a on the regulation of pancreatic cancer cell apoptosis in vitro. These results suggested that Wnt5a is involved in the modulation of pancreatic cancer cell proliferation, and that Wnt5a may be a potential target for pancreatic cancer therapy.


Duan J.,Shanghai University | Yue H.,No 455 Hospital Of Pla | Liu K.,Shanghai University | Wu M.,Shanghai University | Yang J.,Shanghai University
Journal of Medical Colleges of PLA | Year: 2011

Objective: Percutaneous radiofrequency ablation (PRFA) is known to be as effective as hepatectomy for small hepatocellular carcinoma (HCC) in the long-term. We wished to ascertain how it is for recurrent small HCC. Methods: From January 2009 to November 2011, a series of sixty-one patients were included in the study according to the criteria: each patient had one recurrent HCC, less than 5 cm in diameter. Twenty-six of the 61 patients were treated with PRFA and the other 35 were treated with repeat hepatectomy. Results: The interval from first surgery to recurrent for repeat hepatectomy and PRFA groups were (1,239.60±1,017.00) d and (903.42±975.11) d respectively (P=0.066). The tumor-free time after repeat hepatectomy and PRFA were (310.23±159.50) d and (278.27±123.29) d respectively (P=0.584). Size of tumor in repeat hepatectomy and PRFA were (7.34±3.16) cm2 and (5.59±3.40) cm2 (P=0.215), the total expenditure for each patient of the two groups were (26,150.66±7,923.60) yuan and (21,135.00±1,156.76) yuan (RMB), and the time of hospitalization for each of the two groups were (15.29±4.28) d and (7.46±2.20) d (P<0.001). Conclusion: PRFA is proved to be as effective as repeat hepatectomy in the treatment of recurrent small HCC, and superior to repeat hepatectomy as it is less invasive. © 2011 The Editorial Board of Journal of Medical Colleges of PLA. E-edition published by Elsevier (Singapore) Pte Ltd.


PubMed | No 455 Hospital Of Pla and Shanghai University
Type: Journal Article | Journal: Molecular medicine reports | Year: 2016

Wnt proteins are a group of secreted signaling proteins, which function to regulate cell fate and pattern formation during embryogenesis. Altered expression of Wnt5a has been implicated in human carcinogenesis and tumor progression. A previous study identified that Wnt5a is overexpressed in human pancreatic cancer tissues, and that upregulated expression of Wnt5a promotes tumor cell migration and invasion. The present study investigated the role of Wnt5a in pancreatic cancer cell proliferation in vitro and in an orthotopic nude mouse model. Wnt5a cDNA or small interfering RNA were stably transfected into pancreatic cancer cells to assess cell proliferation-associated behaviors, including cell viability, colony formation and apoptosis in vitro, as well as tumor cell growth in an orthotopic nude mouse model. Western blot analysis was used to analyze the expression of Wnt signaling molecules. The data showed that upregulation of the expression of Wnt5a significantly promoted proliferation of the human pancreatic cells, but inhibited tumor cell apoptosis in vitro and promoted tumor growth in an orthotopic nude mouse model. By contrast, knockdown of the expression of Wnt5a inhibited cell growth and promoted apoptosis of the pancreatic cancer cells. The data also revealed that -catenin mediated the effects of Wnt5a on the regulation of pancreatic cancer cell apoptosis in vitro. These results suggested that Wnt5a is involved in the modulation of pancreatic cancer cell proliferation, and that Wnt5a may be a potential target for pancreatic cancer therapy.


Wang W.,No 455 Hospital Of Pla | Xia L.-Q.,No 455 Hospital Of Pla | Chen J.,No 455 Hospital Of Pla | Zhang X.-J.,The Warehouse
Academic Journal of Second Military Medical University | Year: 2014

Objective To establish a LC-MSIMS system for the determination of Bletilla striata glucomannan in Bletilla striata hemostatic sponge. Methods The Waters X Bridge™ Amide(3. 5 [J-ill, 2. 1 mm X 100 mm) column was used at 35 'C; acetonitrile and 5 mmol/L ammonium acetate (containing 0.1% formic acid) (60: 40, VIV) were used as the mobile phase at a flow rate of 0. 3 mLimin, with the injection volume being 10 [J-L. Results The linear range for D-mannose was 50. 0-5 000. 0 nglmL (r=O. 999 5). The results of intra-day and inter-day precisions, limit of detection and limit of quantitation were all within the normal ranges. The recovery rate (n=5) was 98. 4%, RSD= 1. 03%. Conclusion The method in this study is simple, selective and sensitive; it can be used for the quantitative determination and quality control of Bletilla striata glucomannan. © 2014 Second Military Medical University Press. All rights reserved.


Xue X.-C.,No 101 Hospital Of Pla | Liu F.,No 455 Hospital Of Pla
Academic Journal of Second Military Medical University | Year: 2011

Objective To observe the effect of ligustrazine hydrochioridc(LHC) injection on TGF-β1 -induced proliferation and type IV collagen secretion in the human mesangial cells(HMCs). Methods The interstitial fibrosis in kidney disease was mimicked by inducing proliferation and type IV collagen secretion in HMCs with TGF- β1. The experiment was divided into 5 groups: blank, control, low, medium, and high(10, 30, and 100 μg/ml)ligustrazinc hydrochloride groups. MTT method was adopted to examine the proliferation and inhibition rate of HMCs. Enzyme-linked immunosorbent assay was used to determine the production of type IV collagen in cultured HMCs. Results LHC at high concentration (100 μg/ml) significantly inhibited the proliferation of HMCs (P<0. 01). LHC also inhibited the production of type IV collagen, with the significant inhibition found when at the concentration of 100 μg/ml (P


Liu N.-M.,No 455 Hospital Of Pla | Tian J.,No 455 Hospital Of Pla | Wang W.-W.,No 455 Hospital Of Pla | Han G.-F.,No 455 Hospital Of Pla | And 3 more authors.
National Medical Journal of China | Year: 2012

Objective: To explore the effects of erythropoietin (EPO) on the differentiation and secretion of cultured bone marrow-derived mesenchymal stem cells (BM-MSC) in the microenvironment of acute kidney injury (AKI). Methods: C57BL/6 murine BM-MSC (mBM-MSC) were successfully isolated by the methods of Percoll density gradient centrifugation and adherence cultivation. The AKI murine model was induced by ischemia/reperfusion (I/R). The homogenate supernatants were prepared for normal and T/R murine kidney. P3-mBM-MSC were treated differently: Group A: low glucose DMEM medium with 10% fetal bovine serum, Group B; normal murine kidney homogenate supernatant intervention, Group C: T/R kidney homogenate supernatant intervention, Group D; T/R kidney homogenate supernatant plus different concentrations of EPO (1, 5, 10, 50 U/ml). Each group was incubated for 1, 3, 5 and 7 days. Inverted microscope was used to observe the morphological changes of these cells and their ultrastructural changes were observed by transmission electron microscope. Cytokeratin-18 was detected by flow cytometry. The levels of bone morphogenetic protein-7 (BMP-7), hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) were detected by ELISA in culture medium. Results: The cells yielded a high expression of CD29 and CD44 and a low expression of CD34 and CD45. Compared with Groups A and B, the cells of Group C presented oval and short fusiform shapes. After the intervention of EPO, Group D showed a cobble appearance. More organelles were also found. A trace expression of CK18 was found in Groups A and B. A positive expression of CK18 was significantly higher in Groups C and D than Groups A and B(P<0.01). The expression of EPO 50 U/ml at Day 5 and 7 was higher than Group C of the same time (5 d; 35.22±4.04 vs 8.72±0.38, 7 d: 42.00±5.39 vs 13.20±1.14, both P<0.01). The results of ELISA showed that the levels of BMP-7, HGF and VEGF in Group C decreased significantly (P<0.01 or P<0.05). Conclusion The intervention of EPO may boost the differentiation of mBM-MSC but reverse its low secretion.


Wang W.,No 455 Hospital Of Pla | Meng H.,No 85 Hospital Of Pla
Fitoterapia | Year: 2015

Four new spirostane steroidal saponins, (1α,3α)-1-O-[(β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranosyl)]-3-O-d-glucopyranosyl-5α-spirostan (1), (1α,3α)-1-O-[(β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranosyl)oxy]-3-O-d-glucopyranosyl-25(27)-ene-5α-spirostan (2), (1α,3α)-1-O-[(β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranosyl)oxy]-epiruscogenin (3), and (1α,3α)-1-O-[(β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranosyl)oxy]-epineoruscogenin (4) together with two known compounds, bletilnoside A (5) and 3-O-β-d-glucopyranosyl-3-epi-neoruscogenin (6), were isolated from the ethanol extract of the roots of Bletilla striata (Thunb.) Reichb. f. The structures of the isolated compounds were established based on 1D and 2D (1H-1H COSY, HMQC, and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. The isolated compounds were tested in vitro for cytotoxicities against seven tumor cell lines, anti-inflammatory activities against Cox-1 and Cox-2, and hemostatic activities. As a result, compounds 1-4 and 6 exhibited significant cytotoxicities against all the tested tumor cell lines with IC50 value less than 30 μM and selective inhibition of Cox-2 comparable with the standard drug NS-398 (> 90%). Additionally, compounds 1-6 showed potent hemostatic activities. © 2014 Published by Elsevier B.V.


PubMed | No 455 Hospital Of Pla
Type: Journal Article | Journal: Zhonghua yi xue za zhi | Year: 2012

To explore the effects of erythropoietin (EPO) on the differentiation and secretion of cultured bone marrow-derived mesenchymal stem cells (BM-MSC) in the microenvironment of acute kidney injury (AKI).C57BL/6 murine BM-MSC (mBM-MSC) were successfully isolated by the methods of Percoll density gradient centrifugation and adherence cultivation. The AKI murine model was induced by ischemia/reperfusion (I/R). The homogenate supernatants were prepared for normal and I/R murine kidney. P3-mBM-MSC were treated differently: Group A: low glucose DMEM medium with 10% fetal bovine serum, Group B: normal murine kidney homogenate supernatant intervention, Group C: I/R kidney homogenate supernatant intervention, Group D: I/R kidney homogenate supernatant plus different concentrations of EPO (1, 5, 10, 50 U/ml). Each group was incubated for 1, 3, 5 and 7 days. Inverted microscope was used to observe the morphological changes of these cells and their ultrastructural changes were observed by transmission electron microscope. Cytokeratin-18 was detected by flow cytometry. The levels of bone morphogenetic protein-7 (BMP-7), hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) were detected by ELISA in culture medium.The cells yielded a high expression of CD29 and CD44 and a low expression of CD34 and CD45. Compared with Groups A and B, the cells of Group C presented oval and short fusiform shapes. After the intervention of EPO, Group D showed a cobble appearance. More organelles were also found. A trace expression of CK18 was found in Groups A and B. A positive expression of CK18 was significantly higher in Groups C and D than Groups A and B (P < 0.01). The expression of EPO 50 U/ml at Day 5 and 7 was higher than Group C of the same time (5 d: 35.22 4.04 vs 8.72 0.38, 7 d: 42.00 5.39 vs 13.20 1.14, both P < 0.01). The results of ELISA showed that the levels of BMP-7, HGF and VEGF in Group C decreased significantly (P < 0.01 or P < 0.05).The intervention of EPO may boost the differentiation of mBM-MSC but reverse its low secretion.

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