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Nanjing, China

Zhang H.,Shanghai University | Li S.,No. 454 Hospital of PLA | Qu D.,Shanghai University | Qu D.,Center for Disease Control and Prevention of Lanzhou Military Area | And 4 more authors.
International Journal of Cardiology

Aims To assess the efficacy of autologous biological pacing function by autograft of gene-transferred mesenchymal stem cells in a porcine model of complete heart block. Methods and results Fourteen healthy young male pigs were randomized into active group (n = 8) and control group (n = 6). Porcine MSCs were transfected with Ad.HCN4 or Ad.Null. The pacemaker function of transfected MSCs was studied by whole-cell patch clamp. The CHB model of porcine was created with transthoracic ablation technique and the transfected MSCs were autografted into the free wall of right ventricle. The pacing function was studied by ECG and ambulatory Holter recording weekly. The adrenergic responsiveness was evaluated by the variation of heart rate after isoprenaline infusion or food provision following an overnight fasting. HCN4-MSCs expressed a robust time-dependent inward current (If) and the current density of If was 4.3 ± 0.6 pA/pF at - 105 mV. In week 2 after autograft, the heart rate of active group became significantly higher than control (53 ± 5 bpm vs. 38 ± 4 bpm, P < 0.05) and the percent of pacing beats in active group was higher than control (69 ± 10% vs. 28 ± 8%, P < 0.05). By infusion of isoprenaline, the heart rate was increased significantly in both groups. However, there was a significant increase of heart rate when presenting food for active group (P < 0.05) while not in control. Conclusions Our findings demonstrated that autografted HCN4-MSCs could increase the heart rate by providing an adrenergic-responsive biological pacing function, indicating a promising approach without immunological or ethical issues for the treatment of complete heart block. © 2013 Elsevier Ireland Ltd. Source

Lu W.-Q.,Shanghai University | Qiu Y.,No. 454 Hospital of PLA | Pang T.,Shanghai University | Tao X.,Shanghai University | Chen W.-S.,Shanghai University
Academic Journal of Second Military Medical University

Objective: To investigate the effects of S100A6 gene silencing on the proliferation and migration of Eca109 human esophagus cancer cells. Methods: The shRNA expression vectors were constructed using the shRNA sequences designed based on human S100A6's coding sequence, and were transfected into Eca109 cells via cationic liposome. The changes of S100A6 mRNA and protein in Eca109 cells transfected with the recombinant vectors were detected using real-time PCR and Western blotting analysis 48 hours after transfection, respectively; the proliferative curves of transfected cells were plotted using MTT assay; furthermore, the change in cellular migration ability was determined using wound healing assay. Results: The eukaryotic expression vector of shRNA targeting S100A6 was successfully constructed. Real-time PCR and Western blotting analysis results showed that S100A6 was effectively silenced by liposome-mediated transfection of the recombinant shRNA vectors in Eca109 cells. Compared with the untransfected cells, S100A6 mRNA and protein in transfected Eca109 cells were significantly decreased (P<0.05, P<0.01). Meanwhile, the proliferative activity of Eca109 cells was significantly inhibited by S100A6 silencing (P<0.01). It was found that the cellular migration was also suppressed by S100A6 gene interference. Conclusion: S100A6 gene can be effectively silenced by shRNA expression vectors, and the silence may lead to inhibition of the proliferation and migration of Eca109 cells. Source

Cai Y.,Chinese PLA General Hospital | Lu Y.,No. 454 Hospital of PLA | Chen R.,Chinese PLA General Hospital | Wei Q.,Chinese PLA General Hospital | Lu X.,Chinese PLA General Hospital

Different extracts of Rhodobryum giganteum Par. were screened with mice model of acute myocardial hypoxia induced by isoproterenol and pituitrin, eight compounds, i.e. p-hydroxycinnamic acid, caffeic acid-4-O-β-d- glucopyranoside, salicin, 7,8-dihydroxy coumarin, menthol, allantoin, palmitic acid, palmitamide were isolated and identified from the active fraction, among which p-hydroxycinnamic acid and 7,8-dihydroxy coumarin were confirmed having protective effects on myocardial hypoxia-reoxygenation injury, suggesting that these two components were major active ingredients of R. giganteum for cardiovascular disease. © 2010 Elsevier GmbH. Source

Li Z.,Jiangxi University of Traditional Chinese Medicine | Wang Y.,No. 454 Hospital of PLA | Ouyang H.,Jiangxi University of Traditional Chinese Medicine | Lu Y.,No. 454 Hospital of PLA | And 5 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

An ultra performance liquid chromatography (UHPLC) coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS/MS) was used in the structural determination of natural compounds in Gastrodia elata. A total of 64 compounds were identified or tentatively characterized. The strategy used for characterization was comparing their retention time and fragmentation behaviors with those of the reference standards, or investigating their accurate mass measurements and characteristic fragmentation patterns followed by low-energy collision dissociation tandem mass spectrometry (CID-MS/MS). Phenolic conjugates mainly underwent consecutive losses of gastrodin residues and combined losses of H2O and CO2 from their citric acid units under negative MS/MS conditions. According to these rules, we have successfully characterized fifteen potential novel compounds. To confirm the reliability of this strategy, two targeted unknown trace parishins were obtained from G. elata by LC/MS-guided isolation. Based on the analysis of data from NMR spectroscopy and other techniques, the two unknown parishins were identified as 2-[4-O-(β-d-glucopyranosyl)benzyl]-3-methyl-citrate (parishin J) and 1,2-di-[4-O-(β-d-glucopyranosyl)benzyl]-3-methyl-citrate (parishin K), respectively. The fully established structures were consistent with the MS-oriented structural elucidation. This study expanded our knowledge on parishins in Gastrodia species, and the proposed strategy was proven efficient and reliable in the discovery of new minor compounds from herbal extracts. © 2015 Elsevier B.V. Source

Yan D.,No. 454 Hospital of PLA | Zhao B.,No. 454 Hospital of PLA | Yang H.,No. 454 Hospital of PLA | Zhu B.,No. 454 Hospital of PLA | Wang J.,No. 454 Hospital of PLA
Dermatologic Therapy

Keloid is one abnormal wound healing that occurs commonly in predisposed individuals. Many treatment modalities have been tried, either alone or in variety, including surgical removal, laser therapy, radiation therapy, silicone cream or gel application, cryosurgery, and intralesional injection of various agents. These treatment options have been practiced with largely transient and limited success. In the present study, the present authors prospectively assessed the efficacy of combination methods of the treatment of recalcitrant keloid. One hundred fifty-one sites of keloids in 122 patients were chosen, age of patients varied between 15 and 60 years, size of scar more than 2 cm 2 with pain, itch, or other discomfort, and duration of more than 2 years. Keloids were treated with combination methods of lasers and 32P radiation on originating scars. One hundred eleven of 151 evaluated sites scored "excellence" and 40 belonged to "effectiveness". These patients demonstrated local control and have remained free of local recurrence for more than 2 years. In addition, there were only 21 and 32 sites with pigmentation and depigmentation, respectively. Over the past 5 years, we established a treatment protocol combining laser with 32P-patch contact brachyradiotherapy, which represents a safe and effective option in the treatment of keloids. © 2013 Wiley Periodicals, Inc. Source

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