No 422 Hospital Of Pla

Zhanjiang, China

No 422 Hospital Of Pla

Zhanjiang, China
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Fan L.,PLA Fourth Military Medical University | Fan L.,No 210 Hospital Of Pla | Li Y.,No 422 Hospital Of Pla | Sun Y.,PLA Fourth Military Medical University | And 6 more authors.
Phytotherapy Research | Year: 2015

Metastasis is the main cause of death in lung cancer. Targeting the process of metastasis is a strategy to lung cancer treatment. Trillium tschonoskii Maxim., a traditional Chinese medicine, has been used for treatment of many diseases, including cancer. This study aims to determine the anti-metastatic effect of Paris saponin VII (PS VII) which was extracted from T. tschonoskii Maxim. by using human lung cancer cell line A549 cells. Our results showed that PS VII could significantly suppress the viability as well as cell migration and invasion abilities of A549 cells in a concentration-dependent manner. PS VII reduced the activity of matrix metalloproteinase-2 (MMP-2) and MMP-9 by elevating the expression of TIMP1/2. These data indicated that PS VII could reduce the metastatic capability of A549 cells, probably through up-regulating the expression of TIMP1/2. These findings demonstrated a new therapeutic potential for PS VII in anti-metastatic therapy of lung cancer. © 2015 John Wiley & Sons, Ltd. © 2015 John Wiley & Sons, Ltd.


Chen J.,Shanghai University | Wang X.,Shanghai University | Yuan W.,Shanghai University | Tang Y.,Shanghai University | And 2 more authors.
Spine | Year: 2012

STUDY DESIGN.: Case report and literature review. OBJECTIVE.: This article reports 2 cases in which the patients accepted revision surgery after cervical total disc arthroplasty (CTDA) because of iatrogenic neurological injury. SUMMARY OF BACKGROUND DATA.: CTDA has been increasingly investigated to treat cervical degenerative disc disease. However, there are limited reports focused on its complications, especially the neurological complications after the procedure. METHODS.: A 52-year-old man underwent total disc arthroplasty for C5-C6, but immediately after surgery, he experienced paralysis of his upper and lower limbs. Radiographical images indicated residual compression to the spinal cord in the level of C5-C6. Another patient, a 60-year-old man, underwent total disc arthroplasty for C4-C5. Afterward, he experienced severe neck pain and paralysis of upper and lower limbs. He was unresponsive to conservative treatments; thus, a laminectomy was performed 3 months later. However, little improvement was observed. Radiographical images indicated kyphosis and spinal cord compression at the level of C4-C5. Furthermore, both cases showed a high signal in the spinal cord by T2-weighted magnetic resonance image, suggestive of spinal cord injuries. RESULTS.: Revision surgeries were performed in both cases. Cervical implants were first removed by the anterior approach, and fusion was then performed after a complete decompression. Motor examination of the patient in case 1 showed grade 3 strength in both of his hands and feet 6 months after revision surgery. In case 2, the patient's severe neck pain was resolved at the early postoperative stage. Motor examination showed grade 1 strength in both of his hands and feet 3 months after revision surgery. CONCLUSION.: On the basis of presented cases and other reports, the surgical goals in these patients were prioritized as follows: (1) safe and adequate neurological decompression and (2) establishment and maintenance of cervical sagittal balance. Moreover, a criterion for selecting patients undergoing CTDA needs to be established in order to reduce the occurrence of neurological complications associated with the procedure. Copyright © 2012 Lippincott Williams &Wilkins.


Xia L.,PLA Fourth Military Medical University | Xia L.,No 422 Hospital Of Pla | Zhai M.,PLA Fourth Military Medical University | Wang L.,PLA Fourth Military Medical University | And 3 more authors.
Behavioural Brain Research | Year: 2013

Although posttraumatic stress disorder (PTSD) is characterized by traumatic memories or experiences and increased arousal, which can be partly alleviated by antidepressants, the underlying cellular mechanisms are not fully understood. As emerging studies have focused on the critical role of astrocytes in pathological mood disorders, we hypothesized that several 'astrocyte-related' mechanisms underlying PTSD exist. In the present study, using the single prolonged stress (SPS) model, we investigated the effects of intraperitoneal FGF2 on SPS-induced PTSD behavior response as well as the astrocytic activation after FGF2 administration in SPS rats. Behavioral data showed that intraperitoneal FGF2 inhibited SPS-induced hyperarousal and anxiety behavior; however, immunohistochemistry showed that SPS-induced astrocytic inhibition was activated by intraperitoneal FGF2. Quantitative western blotting showed that intraperitoneal FGF2 up-regulated glial fibrillary acidic protein (GFAP), but not NeuN, expression in the hippocampus. We suggest that intraperitoneal FGF2 could block the SPS-induced fear response and anxiety behavior in PTSD via astrocyte-based but not neuron-based mechanisms. © 2013.


An B.,Guangdong Medical University | Zhu B.,No 422 Hospital Of Pla
Chinese Journal of Gastroenterology | Year: 2016

Inflammatory bowel disease (IBD) is a kind of chronic and non-specific inflammatory disease comprising Crohn's disease (CD) and ulcerative colitis (UC), the etiology has not yet been clarified. Endothelin-1 (ET-1) is an active polypeptide composed of 21 amino acid residues, which can constrict blood vessels by activating voltage-dependent Ca2+ channels in vascular smooth muscle cells. Studies have shown that ET-1 plays an important role in the pathogenesis of IBD. This article reviewed the progress in study on ET-1 in the pathogenesis of IBD. COPYRIGHT © 2016 by the Editorial Office of Chinese Journal of Gastroenterology.


Li Y.,No 422 Hospital Of Pla | Li Y.,PLA Fourth Military Medical University | Sun Y.,PLA Fourth Military Medical University | Fan L.,PLA Fourth Military Medical University | And 8 more authors.
Biochemical Pharmacology | Year: 2014

Dysregulation of the Ras signaling pathway plays a key role in the progression of colorectal cancer. When bound to GTP, Ras is activated and stimulates several downstream effectors' pathways, including the Raf/MEK/ERK kinase cascade, the PI3-kinase/AKT/mTor pathway, and the Ral GTPase pathway. Saponins extracted from Liliaceae family herbs have strong antitumor activities with low toxicity. In this study, Paris saponin VII (PSVII), isolated from Trillium tschonoskii Maxim., was evaluated on human colorectal cancer cells (HT-29 and SW-620), a mouse model of colitis associated colorectal cancer (CACC) and a murine model of xenograft tumor. It was found that PSVII inhibited colorectal cancer cell growth in a concentration-dependent manner. The IC 50 values of PSVII for growth inhibition of HT-29 and SW-620 cells were 1.02 ± 0.05 μM and 4.90 ± 0.23 μM. It could induce cell apoptosis, together with cell cycle arrest in G1 phase, and trigger apoptosis in a caspase-3-dependent manner. PSVII-induced growth inhibitory effect was associated with disturbance of MAPK pathway by down-regulating MEK1/2, ERK1/2 phosphorylation, and suppression of AKT pathway by reducing AKT and GSK-3β phosphorylation. In the CACC mouse model, PSVII protected mice from intestinal toxicities and carcinogenesis induced by 1,2-dimethylhydrazine (DMH) and dextran sodium sulfate (DSS). In the model of xenograft tumor, PSVII remarkably decreased the xenograft tumor size and triggered the apoptosis of tumor cells. Both in vitro and in vivo study showed that PSVII inhibited Ras activity. Taken together, PSVII might be a potential therapeutic reagent for colorectal cancer through targeting Ras signaling pathway. © 2014 Elsevier Inc.


Li Y.,No 422 Hospital Of Pla | Li Y.,PLA Fourth Military Medical University | Fan L.,PLA Fourth Military Medical University | Sun Y.,PLA Fourth Military Medical University | And 8 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacological relevance Saponins of several herbs are known to induce apoptosis in some cancer cells and are proposed to be promising modulators of drug resistance. In the present study, we extracted Paris saponin VII (PS VII), a kind of saponin, from Trillium tschonoskii Maxim. and observed its effect on adriamycin-resistant breast cancer cells. Materials and methods An adriamycin-resistant human breast cancer cell line, MCF-7/ADR cells were exposed to different concentrations of PS VII (0-100 μmol/L). Then, flow cytometric assays and a human apoptosis array were used to detect apoptotic cells and apoptosis related protein expression. P-glycoprotein levels and intracellular rhodamine 123 (RH-123) accumulations were measured to evaluate the expression and activity of P-glycoprotein. Results PS VII dose dependently suppressed cell viability as well as triggered apoptosis and modulated drug resistance of MCF-7/ADR cells. Further results showed that PS VII treatment in MCF-7/ADR cells led to increased TNFR1, TRAIL R1/DR4, TRAIL R2/DR5, and FADD expression, and activation of PARP, caspase-8, and 3. In parallel to the alterations, P-glycoprotein expression and activity were also reduced. Conclusion These findings showed that PS VII might be an effective tumouristatic agent for the treatment of MDR breast cancer. © 2014 Elsevier Ireland Ltd.


Niu Y.B.,Northwestern Polytechnical University | Li Y.H.,No 422 Hospital Of Pla | Kong X.H.,Northwestern Polytechnical University | Zhang R.,PLA Fourth Military Medical University | And 7 more authors.
Osteoporosis International | Year: 2012

Summary: The purpose of this study is to investigate the anti-osteoporotic effects of Radix Dipsaci total saponins (RTS). We showed that RTS was able to improve bone properties by either an increase of osteoblastic activity or a decrease in osteoclastic activity. Introduction: Radix Dipsaci has long been used as an antiosteoporotic drug. The present study investigates the antiosteoporotic effects of RTS. Methods: Three-month-old female rats were randomly assigned into a sham-operated group (sham) and five ovariectomy (OVX) subgroups, namely, OVX with vehicle (OVX), OVX with 17β-ethinylestradiol (E2), and OVX with graded doses of RTS (50, 100, or 200 mg/kg/d). RTS and E2 were administered orally, daily from 1 week after OVX treatment for 4 months. Bone mass, turnover, and strength were evaluated by dual-energy X-ray absorptiometry, biochemical markers, and the three-point bending test. The trabecular bone microarchitecture was assessed by microCT. In vitro experiments were performed to determine the potential molecular mechanisms of the anti-osteoporotic effect of RTS. Results: RTS prevented the loss of bone mass induced by OVX. The preventive effect on bone loss was primarily indicated by decreasing levels of bone turnover markers and confirmed by the changes in urinary calcium and phosphorus excretion. The treatment also enhanced the biomechanical strength of bone and prevented the deterioration of trabecular bone microarchitecture. RTS induced MC3T3-E1 and primary osteoblastic cell maturation and differentiation and increased bone formation by increasing BMP-2 synthesis. In addition, RTS inhibited osteoclastogenesis through an increase in osteoprotegrin and a decrease in NF-kB ligand expression in vitro. Conclusions: RTS treatment can effectively suppress the loss of bone mass induced by OVX and in vitro evidence suggests this could be through actions on both osteoblasts and osteoclasts. © International Osteoporosis Foundation and National Osteoporosis Foundation 2012.


PubMed | No 422 Hospital Of Pla and Affiliated Hospital of Guangdong Medical University
Type: | Journal: Computational biology and chemistry | Year: 2016

The PTP non-receptor type 4 (PTPN4) is an important regulator protein in learning, spatial memory and cerebellar synaptic plasticity; targeting the PDZ domain of PTPN4 has become as attractive therapeutic strategy for human neuroglioma. Here, we systematically examined the complex crystal structures of PTPN4 PDZ domain with its known peptide ligands; a number of charged amino acid residues were identified in these ligands and in the peptide-binding pocket of PDZ domain, which can constitute a complicated salt-bridge network across the complex interface. Molecular dynamics (MD) simulations, binding free energy calculations and continuum model analysis revealed that the electrostatic effect plays a predominant role in domain-peptide binding, while other noncovalent interactions such as hydrogen bonds and hydrophobic forces are also responsible for the binding. The computational findings were then used to guide structure-based optimization of the interfacial salt-bridge network. Consequently, five peptides were rationally designed using the high-affinity binder Cyto8-RETEV (RETEV


PubMed | No 421 Hospital Of Pla and No 422 Hospital Of Pla
Type: Journal Article | Journal: The American journal of Chinese medicine | Year: 2016

Crocin, the main effective component of saffron, exerts protective effects against ischemia/reperfusion injury during strokes. However, the effects of crocin in myocardial ischemia/reperfusion injury, and the mechanisms involved, remain unknown. Pretreated with crocin for 7 days, C57BL/6N mice were subjected to 30 min of myocardial ischemia followed by 12[Formula: see text]h of reperfusion (for cardiac function and infarct size, cell apoptosis and necrosis). Neonatal mouse cardiomyocytes were subjected to 2 h of hypoxia followed by 4 h of reoxygenation. NMCMs survival was assessed during hypoxia and reoxygenation in the presence or absence of the autophagy inhibitor 3-methyladenine or the inducer rapamycin. Western blotting was used to evaluate AMPK, Akt, and autophagy-related proteins. Autophagosome was observed using electron microscopy. In the in vivo experiment, crocin pretreatment significantly attenuated infarct size, myocardial apoptosis and necrosis, and improved left ventricular function following ischemia/reperfusion. In vitro data revealed that autophagy was induced during hypoxia, the levels of which were intensely elevated during reoxygenation. Crocin significantly promoted autophagy during ischemia, accompanied with the activation of AMPK.In contrast, crocin overtly inhibited autophagy during reperfusion, accompanied with Akt activation. Induction and inhibition of autophagy mitigated crocin induced protection against NMCMs injury during hypoxia and reoxygenation, respectively. Our data suggest that crocin demonstrated a myocardial protective effect via AMPK/mTOR and Akt/mTOR regulated autophagy against ischemia and reperfusion injury, respectively.


PubMed | No 422 Hospital Of Pla and PLA Fourth Military Medical University
Type: | Journal: BMC developmental biology | Year: 2016

We previously reported that the pluripotent stem cells can differentiate into cardiomyocytes (CMs) by co-culture with neonatal CMs (NCMs) in vitro. However, the involving mechanism is not clear.Mouse induced pluripotent stem cells (iPSCs) were cultured in hanging drops to form embryoid bodies (EBs) and to induce myocardial differentiation. Co-culture of EBs and NCMs was established in a transwell insert system, while EBs grown alone in the wells were used as controls.Co-culture with NCMs markedly increased the generation of functional CMs from iPSCs. The focal adhesion kinase (FAK) phosphorylation, and c-Jun N-terminal kinase (JNK) phosphorylation in co-culture were higher than that in EBs grown alone. Treating FAK small interfering RNA (FAK siRNA) or specific inhibitor for JNK (SP600125) to iPSCs significantly reduced the phosphorylation of JNK and the expressions of Mef2c and Bcl-2. The expressions of cTnT and MLC-2V were also decreased. Our results revealed that co-culture with NCMs significantly enhance the differentiation ability of iPSCs by increasing Mef2c and Bcl-2 expressions concomitantly with a marked augment on cell proliferation through JNK signaling pathways.These findings indicated that co-culture of EBs with NCMs induces genes expressed in a mature pattern and stimulates the proliferation of iPSC-derived CMs (iPS-CMs) by activating FAK/JNK signaling.

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