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Nie W.,Shanghai University | Liu Y.,Shanghai University | Liu Y.,No 411 Hospital Of Pla | Bian J.,Shanghai University | And 2 more authors.
PLoS ONE | Year: 2013

Background: Associations between interleukin-13 (IL-13) polymorphisms and asthma risk remained controversial and ambiguous. Therefore, we performed a meta-analysis to assess the associations between IL-13 polymorphisms and asthma susceptibility. Methods: Pubmed, EMBASE, Chinese National Knowledge Infrastructure (CNKI) and Wangfang databases were searched. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to calculate the strength of association in the random-effects model. Results: Thirty-four studies were included in this meta-analysis. The results indicated that IL13 -1112C/T polymorphism was significantly associated with asthma risk (OR = 1.20, 95% CI 1.08-1.34, P = 0.0009) in a dominant genetic model. When stratifying for race, IL13 -1112C/T polymorphism exhibited increased asthma risk in Caucasians (OR = 1.30, 95% CI 1.09-1.55, P = 0.003), while no significant association was found in Asians and African Americans. In the subgroup analysis based on atopic status, significant association was observed in atopic patients (OR = 1.25, 95% CI 1.07-1.45, P = 0.004) but not in the non-atopic patients. In addition, a significant association between IL13+2044A/G polymorphism and asthma risk was observed (OR = 1.18, 95% CI 1.08-1.28, P = 0.0002). In the subgroup analysis by ethnicity, there were significant associations between IL13+2044A/G polymorphism and asthma risk in Asians (OR = 1.19, 95% CI 1.04-1.36, P = 0.01) and Caucasians (OR = 1.22, 95% CI 1.06-1.40, P = 0.005) but not in African Americans. In the subgroup analysis stratified by atopic status, a marginal significant association was found in atopic patients (OR = 1.12, 95% CI 1.00-1.26, P = 0.05). Conclusions: This meta-analysis suggested that the IL13 -1112C/T and +2044A/G polymorphisms were risk factors for asthma. © 2013 Nie et al.


Huang W.D.,Shanghai University | Huang W.D.,No 411 Hospital Of Pla | Yang X.H.,Shanghai University | Wu Z.P.,Shanghai University | And 8 more authors.
Spine Journal | Year: 2013

Background context: Langerhans cell histiocytosis (LCH) of the spine has been well documented in the literature, but most studies concern management of the disease. No focused report on the differences in clinical and radiographic features of spinal LCH among children, adolescents, and adults exists. Purpose: To review and stress the clinical and imaging differences of spinal LCH in children, adolescents, and adults to avoid false diagnosis. Study design: A retrospective study of children and adults with LCH of the spine. Patient sample: Consecutive patients treated at our institution. Outcome measures: Visual analog scale for pain, Frankel scale for neurologic status, and X-ray, computed tomography (CT) and magnetic resonance imaging (MRI) for imaging. Methods: Seventy-six patients with pathology-proven LCH involving the spine were treated at our institution between 1996 and 2010. Only patients with spine involvement pathologically and/or radiographically were included. Two groups were identified based on the age. Group I comprised children and adolescents (age <18 years; n=40) and Group II comprised adults (age ≥18 years; n=36). Analysis included age and gender distribution, clinical presentation, and imaging features and diagnosis. Pathologic diagnosis was performed by needle or open biopsy of the lesions. Results: Of the 76 patients, 55 were male and 21 were female (ratio of 2.62:1). Neck or back pain was the most common symptom in all patients and was the only presenting symptom in some patients. Restricted motion of spine was the most frequent symptom secondary to pain. Thirty-seven patients presented with neurologic symptoms. Adult patients were more likely to suffer neurologic deficits (p<.005). The distribution of lesions revealed predominance in the cervical spine, followed by thoracic and lumbosacral spine. Plain radiology of children and adolescents with spinal LCH usually revealed a typical vertebral plana, but the adult patients represented different severity of vertebral collapse without typical features. The images of CT scans between the two groups were similar, and all revealed lytic lesions in vertebral bodies and/or posterior elements. In Group I, lesions showed hypointense on T1-weighted images in 15 cases and isointense in 25 cases. Nineteen patients presented as intermediate to slight high signal on T2-weighted images, and the remaining patients presented as hyperintense on T2-weighted images. In Group II, lesions showed hypointense on T1-weighted images in 29 cases, isointense on T1-weighted images in seven cases, and hyperintense on T2-weighted images in 36 cases. Paraspinal soft tissue mass was detected in 28 and 23 cases in Group I and Group II, respectively. Fifteen children and adolescent patients versus 23 adult patients had epidural spinal cord compression. Oversleeve-like or dumbbell sign was observed in 21 cases in Group I but only in four cases in Group II. Conclusions: The most common clinical manifestations of LCH of the spine were neck or back pain, followed by restricted motion of spine, neurologic symptoms, and deformity. Neurologic deficits were more frequent in adult patients. Vertebral plana is the typical imaging feature in children and adolescent patients but seldom in adults. Computed tomography is best for characterizing anatomy of the involved vertebra, and MRI is best for delineating marrow and soft tissue. The oversleeve-like sign on MRI may be a feature of spinal LCH as well as vertebra plana in children and adolescents. Needle biopsy under CT guidance should be performed before a treatment strategy is determined.


Xu W.,Shanghai University | Li X.,Shanghai University | Huang W.,No 411 Hospital Of Pla | Wang Y.,Shanghai University | And 6 more authors.
Annals of Surgical Oncology | Year: 2013

Background: Giant cell tumor (GCT) of the mobile spine is a benign tumor, but it can be potentially aggressive. There is not much published information on GCT of the mobile spine as a result of rarity of the disease, and there are controversies over prognostic factors of the condition. Methods: A retrospective analysis of GCT of the mobile spine was performed by survival analysis. Recurrence-free survival (RFS) was defined as the interval between the date of surgery and the date of recurrence. The postoperative RFS rate was estimated by the Kaplan-Meier method. Factors with P values of ≤0.1 were subjected to multivariate analysis for RFS by proportional hazard analysis. P values of ≤0.5 were considered statistically significant. Results: A total of 102 patients with GCT of the mobile spine were included in the study. The mean follow-up period was 39.9 (median 26.0, range 2-153) months. Thirty-eight patients developed recurrence. The univariate and multivariate analysis suggested that age less than 40 years, total spondylectomy either by en bloc or piecemeal method, and administration of bisphosphonate were independent favorable prognostic factors. Subgroup analysis by excluding patients before the year 2000 further confirmed our findings. Conclusions: The removal of the entire osseous compartment either by en bloc or piecemeal method in combination with the long-term use of bisphosphonate could significantly reduce the recurrence rate of GCT of the mobile spine. Age less than 40 years is a favorable prognostic factor for GCT in the mobile spine. © 2012 Society of Surgical Oncology.


Cai W.,Shanghai University | Yan W.,Shanghai University | Huang Q.,Shanghai University | Huang W.,No 411 Hospital Of Pla | And 2 more authors.
European Spine Journal | Year: 2015

Purpose: Multiple myeloma (MM) and solitary plasmacytoma of bone (SPB) are two independent subtypes of plasma cell dyscrasias which often occur in spine. However, little is known about the surgical treatment of patients with spinal instability or neurological impairment caused by spinal lesions as the first clinical manifestation. The present study aimed to investigate the surgical outcome of these patients. Methods: We retrospectively reviewed the data of a total of 64 patients receiving spinal surgery in our center, in which 30 were diagnosed as MM and 34 as SPB. Univariate and multivariate analyses were used to identify factors associated with overall survival (OS) and progression-free survival (PFS) of patients. Results: Surgical treatment led to favorable results including pain relief, resumption of ambulatory ability as well as improvement of neurological function and life quality. Univariate analysis suggested that the potential prognostic factors for OS of MM patients were bisphosphonate treatment, post-surgical ambulatory status, Karnofsky Performance Score (KPS) and Frankel scale, and for PFS of MM patients were age at surgery, resection mode, postoperative ambulation status, KPS and Frankel scale, while the PFS of SPB patients was only significantly related to postoperative adjuvant therapies. Multivariate analysis indicated that postoperative ambulation status was the only independent risk factor for both OS and PFS of MM patients. Conclusions: Surgery may be beneficial to patients with spinal instability or neurological impairment caused by spinal lesions as the first clinical manifestation, in which MM patients with postoperative ambulatory ability display better prognosis. © 2014, Springer-Verlag Berlin Heidelberg.


Zhang J.,Shanghai University | Zuo C.-J.,Shanghai University | Jia N.-Y.,Shanghai University | Wang J.-H.,Ningbo University | And 5 more authors.
World Journal of Gastroenterology | Year: 2015

AIM: To explore the diagnostic value of the cross-modality fusion images provided by positron emission tomography/computed tomography (PET/CT) and contrast-enhanced CT (CECT) for pancreatic cancer (PC). METHODS: Data from 70 patients with pancreatic lesions who underwent CECT and PET/CT examinations at our hospital from August 2010 to October 2012 were analyzed. PET/CECT for the cross-modality image fusion was obtained using TureD software. The diagnostic efficiencies of PET/CT, CECT and PET/CECT were calculated and compared with each other using a χ2 test. P < 0.05 was considered to indicate statistical significance. RESULTS: Of the total 70 patients, 50 had PC and 20 had benign lesions. The differences in the sensitivity, negative predictive value (NPV), and accuracy between CECT and PET/CECT in detecting PC were statistically significant (P < 0.05 for each). In 15 of the 31 patients with PC who underwent a surgical operation, peripancreatic vessel invasion was verified. The differences in the sensitivity, positive predictive value, NPV, and accuracy of CECT vs PET/CT and PET/CECT vs PET/CT in diagnosing peripancreatic vessel invasion were statistically significant (P < 0.05 for each). In 19 of the 31 patients with PC who underwent a surgical operation, regional lymph node metastasis was verified by postsurgical histology. There was no statistically significant difference among the three methods in detecting regional lymph node metastasis (P > 0.05 for each). In 17 of the 50 patients with PC confirmed by histology or clinical follow-up, distant metastasis was confirmed. The differences in the sensitivity and NPV between CECT and PET/CECT in detecting distant metastasis were statistically significant (P < 0.05 for each). CONCLUSION: Cross-modality image fusion of PET/CT and CECT is a convenient and effective method that can be used to diagnose and stage PC, compensating for the defects of PET/CT and CECT when they are conducted individually. © The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.


Tong Q.,Shanghai University | Zhao L.,Shanghai University | Qian X.-D.,No 411 Hospital Of Pla | Zhang L.-L.,Shanghai University | And 4 more authors.
Pharmacogenomics | Year: 2013

Aim: To explore whether TNF-α promoter -308 A/G and -857 C/T polymorphisms have an association with responsiveness to TNF blockers in spondyloarthritis and inflammatory bowel disease. Methods: A meta-analysis was performed. Pooled odds ratios (ORs) and 95% CIs were calculated. Results: Six relevant studies with a total of 211 spondyloarthritis patients and 392 inflammatory bowel disease patients were included. The results showed that the common allele (G and C, respectively) showed a better responsiveness than the minor allele (A and T, respectively). The -308 G/G genotype (OR: 2.31; 95% CI: 1.36-3.91; p = 0.002) and -857 C/C genotype (OR: 3.66; 95% CI: 1.35-9.92; p = 0.01) responded better to therapy, which was different from the results of some studies included. Conclusion: Individuals with the TNF-α-308 G allele and -857 C allele showed better anti-TNF-α treatment responses than those with the TNF-α-308 A allele and -857 T allele. The -308 G/G genotype and -857 C/C genotype are predictors of good response. Original submitted 30 May 2013; Revised submitted 25 July 201. © 2013 Future Medicine Ltd.


PubMed | No 411 Hospital Of Pla and Shanghai University
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2016

Anti-glomerular basement membrane (GBM) disease is characterized by circulating anti-GBM antibodies and deposition of these antibodies in the renal GBM. Renal involvement in anti-GBM is more severe when compared with other types of immune-mediated glomerulonephritis, and the majority of patients manifest progressive renal failure, leading to end-stage renal disease. In a limited number of cases, anti-GBM disease has been shown to be accompanied with other immune-mediated glomerulonephritis. The present study reported the case of a 50-year-old female patient presenting with rapidly progressive glomerulonephritis, who was diagnosed with anti-GBM disease with IgA nephropathy. The patient achieved a relatively good therapeutic outcome with administration of corticosteroids plus mycophenolate mofetil (MMF), which may prove to be a novel treatment option for this rare disease; however, the exact underlying mechanism requires further in-depth investigation.


PubMed | Henan University, No 411 Hospital Of Pla and Shanghai University
Type: Journal Article | Journal: Journal of Cancer | Year: 2016

Hedgehog(HH) pathway is found to be activated through a manner of canonical, or the non-canonical HH pathways. Distinct hyperplasia stroma around tumor cells is supposed to express pro-inflammatory cytokines abundantly, such as tumor necrosis factor- (TNF-) and interleukin-1 (IL-1), etc. in pancreatic ductal adenocarcinoma (PDAC) tissues. In this study we observed the effects of TNF- and IL-1 on HH pathway activation in PDAC cells, and explored their activation manners. Our results showed that pro-inflammatory cytokines, TNF- and IL-1, could up-regulate the expression of GLI1 gene, increase its nuclear protein expression and promote malignant cell behaviors including migration, invasion, epithelial-mesenchymal transition (EMT) and drug resistance as well. Moreover, GLI1 promoter-reporter assay in combination with blocking either NF-B or Smoothened (SMO) suggested that TNF- and IL-1 could transcriptionally up-regulate expression of GLI1 completely via NF-B, whereas ablation of SMO could not completely attenuate the regulation effects of TNF- and IL-1 on GLI1 expression. Collectively, our results indicated that TNF- and IL-1 in hyperplasia stroma can promote the PDAC cell development by activating HH pathway, through both the canonical and non-canonical HH activation ways.


Zhou J.G.,No 411 Hospital Of Pla
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology | Year: 2012

To investigate the effect of hyperbaric oxygen (HBO) treatment on the expression of nitric oxide synthase (NOS) mRNA in cortex after acute traumatic cerebral injury, and to study the mechanism of HBO on brain injury. Acute traumatic brain injury model was established with rest received free fall injury method in SD rats. 0.25 MPa HBO treatment was used 1 h or 12 h after brain injury and the cortex was isolated 6 h or 24 h after brain injury respectively. The expression of mRNA coding for nNOS, eNOS or iNOS were assayed using reverse transcription polymerase chain reaction (RT-PCR). The expression of nNOS, eNOS and iNOS mRNA were significantly decreased in 0.25 MPa HBO treatment groups than those in acute cerebral injury groups (P < 0.01). The amount of nNOS, eNOS and iNOS mRNA was significantly lower in HBOT 24 h group than those in HBOT 6 h group (P < 0.05, P < 0.01). There was no significantly difference among nNOS, eNOS and iNOS mRNA in 0.25 MPa normoxic hyperbaric nitrogen groups and acute cerebral injury groups (P > 0.05). HBO may exert significant effects on the expression of nNOS mRNA/iNOS mRNA and protect cortical neuronal from traumatic cerebral injury.


Liu Y.,No 411 Hospital Of Pla | Zhuo A.,No 411 Hospital Of Pla | Liu W.,No 411 Hospital Of Pla | Xu S.,No 411 Hospital Of Pla | Li S.,No 411 Hospital Of Pla
Journal of Investigational Allergology and Clinical Immunology | Year: 2014

Objective: The association between the interleukin 4 (IL-4) gene -33C/T polymorphism and asthma risk is a subject of debate. We conducted a meta-analysis to evaluate the association between this polymorphism and asthma susceptibility. Materials and Methods: A systematic search of electronic databases (Pubmed, EMBASE, Wanfang, China National Knowledge Infrastructure, and Weipu) was performed, and 18 studies involving 5523 cases and 5618 controls were identified. ORs with 95% CIs were used to assess the strength of association. Results: A significant association between the -33C/T polymorphism and asthma susceptibility was observed for TT vs CT + CC (OR, 1.16; 95% CI, 1.05-1.28; P=.005). In the subgroup analysis by race, a significant association was found among whites (OR, 1.71; 95% CI, 1.14-2.57; P=.01) and Asians (OR, 1.14; 95% CI, 1.01-1.28; P=.04) but not among African Americans. In the subgroup analysis by atopic status, no significant association was found among atopic asthma patients (OR, 1.05; 95% CI, 0.89-1.24; P=.54) or nonatopic asthma patients (OR, 1.16; 95% CI, 0.81-1.67; P=.42). In the age-stratified analysis, an increased asthma risk was found in children (OR, 1.28; 95% CI, 1.01-1.63; P=.04) but not in adults. Conclusions: The results of this meta-analysis suggest that the IL-4 -33C/T polymorphism is a risk factor for asthma. © 2014 Esmon Publicidad.

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