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Gao Y.,No. 411 Hospital of CPLA | Jing M.,No. 411 Hospital of CPLA | Ge R.,No. 411 Hospital of CPLA | Lang L.,Tongji University
Molecular and Cellular Biochemistry | Year: 2016

Alternations of environment signals such as neurotrophins may be the basis for malignant transformation of retinoblastoma (Rb), the most common primary intraocular malignancy in children. The aim of this study is to investigate the ability of brain-derived neurotrophic factor (BDNF) to decrease the chemosensitivity of Rb cells to the common chemotherapeutic agents and to explore the role of hypoxia-inducible factor-1α (HIF-1α) in such cellular process. The results showed that BDNF could induce higher expression of HIF-1α via activation of TrkB in human Y-79 retinoblastoma cells, which consequently contributed to its effect against chemotherapeutic agent-induced cytotoxicity and cell apoptosis. However, this protective effect could be potently reversed by knockdown of HIF-1α. Furthermore, BDNF strikingly prevented chemotherapeutic agent-induced alternations of apoptosis-related molecules, which could also be attenuated by silencing HIF-1α. Therefore, our findings demonstrated that BDNF could contribute to chemoresistance of Rb via modulation of HIF-1α expression, indicating that targeting at the BDNF-TrkB/HIF-1α signaling pathway might be a promising strategy for the treatment of retinoblastoma in the future. © 2016 Springer Science+Business Media New York Source

Gao Y.,No. 411 Hospital of CPLA | Jing M.,No. 411 Hospital of CPLA | Ge R.,No. 411 Hospital of CPLA | Zhou Z.,No. 411 Hospital of CPLA | Sun Y.,No. 411 Hospital of CPLA
Journal of Biochemical and Molecular Toxicology | Year: 2014

Hypoxia, which activates the hypoxia inducible factor 1α (HIF-1α), is an essential feature of retinoblastoma (RB) and contributes to poor prognosis and resistance to conventional therapy. In this study, the effect of HIF-1α knockdown by small interfering RNA (siRNA) on cell proliferation, apoptosis, and apoptotic pathways of human Y-79 RB cells was first investigated. Exposure to hypoxia induced the increased expression of HIF-1α both in mRNA and protein levels. Then, knockdown of HIF-1α by siRNAHIF-1α resulted in inhibition of cell proliferation and induced cell apoptosis in human Y-79 RB cells under both normoxic and hypoxic conditions, with hypoxic conditions being more sensitive. Furthermore, knockdown of HIF-1α could enhance hypoxia-induced slight increase of Bax/Bcl-2 ratio and activate caspase-9 and caspase-3. These results together indicated that suppression of HIF-1α expression may be a promising strategy for the treatment of human RB in the future. © 2014 Wiley Periodicals, Inc. Source

Zhou J.,No. 411 Hospital of CPLA | Liu C.,No. 411 Hospital of CPLA | Shan P.,No. 411 Hospital of CPLA | Zhou Y.,No. 411 Hospital of CPLA | And 2 more authors.
Journal of Diabetes and its Complications | Year: 2013

Objective: The prevalence and clinical features of masked hypertension (MH) in type 2 diabetes mellitus patients (T2DM) were investigated to define clinical indices which may aid diagnosis and treatment. Methods: Clinical blood pressure (CBP) and ambulatory blood pressure (ABP) were measured in 856 T2DM patients to differentiate normotensive (NT), essential hypertensive (EH), and MH. Waist circumference (WC), abdominal circumference (AC), body mass index (BMI), waist to height ratio (WHtR), fasting blood glucose (FBG), and glycated hemoglobin levels were measured and compared between BP groups. Results: In total, 359 patients had normal CBP, of which 13.37% were diagnosed with MH based on established criteria. Males had significantly higher rates of MH (15.30%) than females (11.36%) (P = 0.036). The MH detection rate increased with age and T2DM duration. There were no significant differences in BMI, WC or WHtR between total MH and EH groups. MH females, however, had lower BMIs than female EH females (P = 0.023). Smoking, alcohol, and familial EH history were lower in MH than EH patients (smoking, P = 0.029; alcohol,P = 0.001; and EH history, P = 0.000), while BMI (male, P = 0.037, female, P = 0.015), WC (male, P = 0.012, female, P = 0.021), WHtR (P = 0.011), smoking (P = 0.016), and alcohol consumption (P = 0.000) were higher in MH than NT patients. BMI, WHtR, 6-15 year disease course of diabetes, smoking and alcoholism were independent risk factors of T2DM complicated with MH. The dipper BP circadian pattern was significantly lower in MH than NT patients (P = 0.001). The non-dipper pattern was lower in MH than EH (P = 0.018) but higher than in NT (P = 0.000). Conclusions: A significant fraction of T 2DM patients were diagnosed with MH. Clinical presentation also contrasted sharply from EH, MH is a specific blood pressure status that may severely damage target organs in T2DM. © 2013 Elsevier Inc. Source

Liu C.,No. 411 Hospital of CPLA | Zhou J.,No. 411 Hospital of CPLA | Yang X.,No. 411 Hospital of CPLA | Lv J.,No. 411 Hospital of CPLA | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Objectives: We examined changes in sleep quality and architecture in patients with minimal hepatic encephalopathy (MHE) and the impacts of sleep disruption on patient physical and psychological health. Methods: Ninety-eight MHE patients were examined by polysomnography (PSG) and the Pittsburg sleep quality inventory (PSQI). In addition, patients completed the SAS, SDS, and SCL-90 to examine the relationship between sleep quality and psychological health. Results: Mean relative durations of Stage 1 and Stage 2, sleep latency, microarousal frequency, and total sleep time (TST) were all lower in MHE patients compared to healthy controls (P<0.05 for all). Similarly, SWS and REM stage durations, REM latency, sleep maintenance rate, and sleep efficiency were lower than controls (P<0.01 for all). Mean PSQI scores were lower in MHE patients. Total SAS, SDS, and SCL-90 scores, as well as all SCL-90 subscores, were significantly higher in the MHE group (P<0.05), indicating significant psychological dysfunction. Longer SWS, longer REM, and lower microarousal frequency were associated with improved sleep quality (P<0.05), while shorter SWS and REM led to dyssomnia and daytime functional disturbance (P<0.05, P<0.01). Longer REM latency and higher microarousal frequency were associated with higher PSQI scores (P<0.05, P<0.01), while longer SWS, longer REM, and higher sleep maintenance rate were associated with lower PSQI scores (P<0.05, P<0.01). Finally, total PSQI score and sleep efficiency subscore were positively correlated with total SCL-90 and most SCL-90 subscores (P<0.05). Conclusions: MHE patients suffer from multiple subjective dyssomnias and changes in sleep architecture that are strongly correlated with psychological dysfunction. © 2015, E-Century Publishing Corporation. All rights reserved. Source

Zhou J.,No. 411 Hospital of CPLA | Liu C.,No. 411 Hospital of CPLA | Shan P.,No. 411 Hospital of CPLA | Zhou Y.,No. 411 Hospital of CPLA | And 2 more authors.
Clinical and Experimental Hypertension | Year: 2014

Objectives: This study documented the prevalence and clinical features of white coat hypertension (WCH) among Chinese Han patients with type 2 diabetes mellitus (T2DM). Methods: Clinic and ambulatory blood pressure (BP) measurements were compared in 856 patients with T2DM to determine the frequency of WCH (WCH was defined as clinical blood pressure 140/90mmHg and daytime blood pressure <135/85mmHg and/or 24-h ambulatory BP (ABP) mean value of <130/80mmHg on ambulatory BP monitoring (ABPM). Weight, waist circumference (WC), body mass index (BMI), waist to height ratio (WHtR), fasting blood glucose, glycosylated hemoglobin level and circadian BP patterns were also measured to find clinical features predictive of WCH in T2DM. Results: The prevalence of WCH was 7.36% (63/856) in the overall population, 6.13% (29/473) in male and 8.88% (34/383) in female (p<0.05). WCH accounted for 14.03% (63/449) of diagnosed hypertension. Age, course of T2DM, male WC were independent protective factors, whereas female sex, smoking and alcohol consumption were independent risk factors for WCH in T2DM. Non-dippers and reverse dippers made up larger proportion of the WCH group (p<0.01). Conclusion: WCH is relatively common among T2DM patients, it is a unique condition distinct from essential hypertension (EH), and WCH patients also exhibit significant differences in clinical parameters. © 2014 Informa Healthcare USA, Inc. Source

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