Chongqing, China
Chongqing, China

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Zhong Y.,No 324 Hospital Of Pla | Liu Z.,Chongqing Medical University
Chinese Journal of Medical Imaging Technology | Year: 2015

Objective: To explore the pathological mechanism of microbubbles mediated ultrasound cavitation obstructed tumor microcirculation. Methods: Totally 18 New Zealand rabbits bearing liver VX2 tumor were randomly divided into 3 groups (each n=6). The microbubbles injection and ultrasound irradiation were performed in mirobubbles group (US+MB group). The normal saline injection and ultrasound irradiation were performed in simple ultrasound group (US group). And the normal saline injection and sham ultrasonic irradiation were performed in sham-irradiated group. The pathological changes were observed. Results: The gross anatomy showed significant hemorrhage of target region in US+MB group, while no significant changes in US and sham-irradiated groups. Pathological observation with light microscope showed significant hemorrhage, erythrocyte spillover, vascular endothelial interrupt and cells injured in US+MB group; few hemorrhage and mild injury of endothelium in US group; unbroken endothelium and insignificant hemorrhage in sham-irradiated group. Pathological observation with electronic microscope showed badly broken endothelium, significant hemorrhage; swelling of mitochondrion and pinocytosis vesicles in US+MB group, only edema of endoplasmic reticulum in US and sham-irradiated groups. Conclusion: The pathological mechanism of microbubbles mediated ultrasound cavitation on tumor microcirculation obstruction maybe vascular mechanical damage. Copyright © 2015 by the Press of Chinese Journal of Medical Imaging and Technology.


Zhu X.,Southwest University | Jiang M.,The Ninth Peoples Hospital of Chongqing | Song E.,Southwest University | Jiang X.,No 324 Hospital Of Pla | Song Y.,Southwest University
Food and Chemical Toxicology | Year: 2015

Ultraviolet B (UVB) radiation causes oxidative damage and inflammation, and ultimately increases the risk of skin carcinogenesis. Selenium is an essential trace element, previous studies indicated selenium deficiency impairs tissue antioxidant capacity in different experimental models. However, the synergistic effect of selenium deficiency and UVB radiation on skin damage is not clear. In the current study, our data revealed selenium deficiency resulted in further increases of reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS) and phosphorylated H2AX levels, decreases of GSH level and antioxidant enzyme activities in UVB-irradiated mice. Selenium deficiency also exacerbated UVB-induced cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and IL-6 mRNA expressions. Mechanism studies indicated that UVB-induced p38 signaling was further elevated in the skin of mice maintained with selenium deficiency diet, compared with those maintained with selenium adequate diet. Our investigation suggested that selenium deficiency diet weakens the antioxidant capacity of UVB-irradiated mice skin, which sensitizes to UV radiation-induced oxidative damage and inflammation. © 2014 Elsevier Ltd.


PubMed | No 324 Hospital Of Pla, The Ninth Peoples Hospital of Chongqing and Southwest University
Type: | Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Year: 2015

Ultraviolet B (UVB) radiation causes oxidative damage and inflammation, and ultimately increases the risk of skin carcinogenesis. Selenium is an essential trace element, previous studies indicated selenium deficiency impairs tissue antioxidant capacity in different experimental models. However, the synergistic effect of selenium deficiency and UVB radiation on skin damage is not clear. In the current study, our data revealed selenium deficiency resulted in further increases of reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS) and phosphorylated H2AX levels, decreases of GSH level and antioxidant enzyme activities in UVB-irradiated mice. Selenium deficiency also exacerbated UVB-induced cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNF-), interleukin-1beta (IL-1) and IL-6 mRNA expressions. Mechanism studies indicated that UVB-induced p38 signaling was further elevated in the skin of mice maintained with selenium deficiency diet, compared with those maintained with selenium adequate diet. Our investigation suggested that selenium deficiency diet weakens the antioxidant capacity of UVB-irradiated mice skin, which sensitizes to UV radiation-induced oxidative damage and inflammation.


Chen G.,No 324 Hospital Of Pla | Li X.-C.,No 324 Hospital Of Pla | Wu G.-Q.,No 324 Hospital Of Pla | Zhang S.-X.,Chongqing Medical University | And 6 more authors.
Chinese Medical Journal | Year: 2010

Background: Comparing with two dimensional (2D) imaging, both in diagnosis and treatment, three dimensional (3D) imaging has many advantages in clinical medicine. 3D reconstruction makes the target easier to identify and reveals the volume and shape of the organ much better than 2D imaging. A 3D digitized visible model of the liver was built to provide anatomical structure for planing of hepatic operation and for realizing accurate simulation of the liver on the computer. Methods: Transverse sections of abdomen were chosen from the Chinese Visible Human dataset. And Amira software was selected to segment and reconstruct the structures of the liver. The liver was reconstructed in three-dimensions with both surface and volume rendering reconstruction. Results: Accurately segmented images of the main structures of the liver were completed. The reconstructed structures can be displayed singly, in small groups or as a whole and can be continuously rotated in 3D space at different velocities. Conclusions: The reconstructed liver is realistic, which demonstrates the natural shape and exact position of liver structures. It provides an accurate model for the automated segmentation algorithmic study and a digitized anatomical mode of viewing the liver.


Hao L.,No 324 Hospital Of Pla | Sun H.,Chongqing Medical University | Wang J.,Daping Hospital | Wang T.,Chongqing Medical University | And 2 more authors.
International Journal of Hematology | Year: 2012

Mesenchymal stromal cells (MSC) have attracted the attention of scientists and clinicians due to their self-renewal, capacity for multipotent differentiation, and immunomodulatory properties. Some essential problems remain to be solved before the clinical application of MSC. Platelet lysate (PL) has recently been used as a substitute for FBS in MSC amplification in vitro to achieve clinically applicable numbers of MSC. In addition to promising trials in regenerative medicine, such as in the treatment of major bone defects and myocardial infarction, MSC have shown therapeutic effect other than direct hematopoiesis support in hematopoietic reconstruction. It has been confirmed that MSC promote hematopoietic cell engraftment and immune recovery after allogeneic hematopoietic stem cell transplantation, probably through the provision of cytokines, matrix proteins, and cell-to-cell contacts. Their suppressive effects on immune cells, including T cells, B cells, NK cells and DC cells, suggest MSCs as a novel therapy for GVHD and other autoimmune disorders. These cells thus present as promising candidates for cellular therapy in the fields of regenerative medicine, allogeneic hematopoietic stem cell transplantation, and autoimmune disorders. © 2011 The Japanese Society of Hematology.


Hao L.,No 324 Hospital Of Pla | Zou Z.,Beijing Research Institute of Chemical Defense | Tian H.,No 324 Hospital Of Pla | Zhang Y.,No 324 Hospital Of Pla | And 3 more authors.
Neurological Sciences | Year: 2015

Neovascularization, including angiogenesis, arteriogenesis and vasculogenesis, occurs in physiological and pathological state. Efficient and controlled reconstruction of functional vascular network is necessary. Nerves and blood vessels establish intricate branching network in every organ which share anatomical and functional characteristics. Nerves direct vessel branching patterns and arterial differentiation in the developmental embryonic limb skin. Various neurotransmitters exert antiapoptotic, chemotactic and proliferative effect on EC and SMC, and modulate all the phases of neovascularization. Hence, we proposed that the perivascular nerves may play critical roles in neovascularization. © 2014, Springer-Verlag Italia.


Xia X.,Southwest University | Su C.,Southwest University | Fu J.,Southwest University | Zhang P.,Southwest University | And 5 more authors.
International Immunopharmacology | Year: 2014

This study investigated the protective effect of α-lipoic acid (LA) on lipopolysaccharide (LPS)/d-galactosamine (d-GalN)-induced fulminant hepatic failure in mice. First, we found that LA markedly reduced LPS/d-GalN-induced increases in serum ALT and AST activities, which were supplemented with histopathological examination, suggested that LA has a protective effect on this model of hepatic damage. Livers challenged with LPS/d-GalN exhibited extensive areas of vacuolization with the disappearance of nuclei and the loss of hepatic architecture. On the contrary, these pathological alterations were ameliorated by LA treatment. Next, we found that ROS and TBARS levels were increased in LPS/d-GalN treated liver homogenates, which were attenuated by LA administration. Consistently, decreases in hepatic CAT and GPx activities were observed in LPS/d-GalN group and were significantly restored by LA administration. Moreover, pretreatment with LA markedly reduced LPS/d-GalN-induced iNOS, COX-2, TNF-α, NF-κB, IL-1β and IL-6 expressions. Furthermore, our data showed that TUNEL-positive cells increased in LPS/d-GalN-treated mice liver which was counteracted by LA administration. LPS/d-GalN induced apoptosis of hepatocytes, as estimated by caspase 3, caspase 8 and caspase 9 activations. Also, the increasing of Bax and the decreasing of Bcl-2 expressions also supported LPS/d-GalN induced apoptosis. Interestingly, LA marked relieved these apoptotic features. Taking together, our results indicated that LA plays an important role on LPS/d-GalN-induced fulminant hepatic failure through its antioxidant, anti-inflammatory and anti-apoptotic activities. © 2014 Elsevier B.V.


Jiang X.,Chongqing Medical University | Guo X.,Chongqing Medical University | Xu X.,No 324 Hospital Of Pla | Teng M.,Chongqing Medical University | And 5 more authors.
Scientific Reports | Year: 2014

Keratinocyte migration is an early event in the wound healing process. Although we previously found that CD9 downregulation is required for the keratinocyte migration during wound repair, the mechanism of how CD9 expression is regulated remains unclear. Here, we observed the effect of hypoxia (2% O 2) on CD9 expression and keratinocyte migration. CD9 expression was downregulated and keratinocyte migration was increased under hypoxic conditions. In addition, CD9 overexpression reversed hypoxia-induced cell migration. We also found that hypoxia activated the p38/MAPK pathway. SB203580, a p38/MAPK inhibitor, increased CD9 expression and inhibited keratinocyte migration under hypoxia, while MKK6 (Glu) overexpression decreased CD9 expression and promoted hypoxic keratinocyte migration. Our results demonstrate that hypoxia regulates CD9 expression and CD9-mediated keratinocyte migration via the p38/MAPK pathway.


Wang T.,Chongqing Medical University | Feng Y.,Chongqing Medical University | Sun H.,Chongqing Medical University | Zhang L.,Chongqing Medical University | And 8 more authors.
American Journal of Pathology | Year: 2012

With the clarification of the important roles of microRNAs (miRNAs) in diverse physiologic and pathologic processes, the effects of miRNAs in wound healing have attracted more attention recently. However, the global pattern of miRNA expression in wound tissue is still unknown. In the present study, we depicted the miRNA profile and identified at least 54 miRNAs, including miR-21, changed for more than twofold at the stage of granulation formation during wound healing. These miRNAs were closely related to the major events of wound healing, including cell migration and proliferation, angiogenesis, and matrix remolding. Furthermore, we found that miR-21 was up-regulated after skin injury, mainly in activated and migrating epithelial cells of epidermis and mesenchymal cells of dermis. Locally antagonizing miR-21 by directly injecting antagomir to wound edge caused significant delay of wound closure with impaired collagen deposition. Unexpectedly, we found wounds treated with miR-21 antagomir had an obvious defect in wound contraction at an early stage of wound healing. The significant role of miR-21 in wound contraction was further confirmed by in vivo gain-of-function and in vitro loss-of-function experiments. In conclusion, the present study has for the first time depicted miRNA profiling of wound healing and demonstrated the involvement of miR-21 in regulating the wound contraction and collagen deposition. These results suggest that miR-21 may be a new medical target in skin wound manipulation. © 2012 American Society for Investigative Pathology.


PubMed | No 324 Hospital of PLA
Type: Journal Article | Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] | Year: 2013

Endothelial progenitor cells (EPCs) are defined as a special type of stem cell that have been found to directly incorporate into injured vessels and that participate in angiogenesis and reconstruction by differentiation into endothelial cells. EPCs are widely used to therapeutically treat cardiovascular disease, limb ischemia and vascular repair. However, the role of EPCs in inflammatory diseases, especially in lung injury, is less studied.To investigate the application of EPCs to vascular repair, and the role of EPCs in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS).A computer-based online search was performed in the PubMed database and Web of Science database for articles published, concerning EPCs, angiogenesis, ALI/ARDS and stem cell transplantationEPCs have a therapeutic potential for vascular regeneration and may emerge as novel strategy for the diseases that are associated with ALI/ARDS.

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