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Chongqing, China

Cai Y.,No.324 Hospital of PLA
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology | Year: 2010

To identify the HBV genotype-specific tag sequence. The large S region sequences from 930 HBV genomes were aligned to identify the genotype-specific tag sequences. PCR was used to check the genotyping effect of these tags. Two tag sequences, sequence between 149-169 and sequence between 461-483, were identified in the large S region. Using primers specific to these tag sequences, the genotype of HBV can be specifically identified. These tag sequences can be used for HBV genotyping. Source


Guo L.,Chongqing Medical University | Li S.-Y.,Kunming General Hospital of Chengdu Military Command | Ji F.-Y.,Chongqing Medical University | Zhao Y.-F.,Pudong New Area Gongli Hospital | And 4 more authors.
Inflammation Research | Year: 2014

Background: Angptl4 is a secreted protein involved in the regulation of vascular permeability, angiogenesis, and inflammatory responses in different kinds of tissues. Increases of vascular permeability and abnormality changes in angiogenesis contribute to the pathogenesis of tumor metastasis, ischemic-reperfusion injury. Inflammatory response associated with Angptl4 also leads to minimal change glomerulonephritis, wound healing. However, the role of Angptl4 in vascular permeability, angiogenesis, and inflammation is controversy. Hence, an underlying mechanism of Angptl4 in different kind of tissues needs to be further clarified. Methods: Keywords such as angptl4, vascular permeability, angiogenesis, inflammation, and endothelial cells were used in search tool of PUBMED, and then the literatures associated with Angptl4 were founded and read. Results: Data have established Angptl4 as the key modulator of both vascular permeability and angiogenesis; furthermore, it may also be related to the progression of metastatic tumors, cardiovascular events, and inflammatory diseases. This view focuses on the recent advances in our understanding of the role of Angptl4 in vascular permeability, angiogenesis, inflammatory signaling and the link between Angptl4 and multiple diseases such as cancer, cardiovascular diseases, diabetic retinopathy, and kidney diseases. Conclusions: Taken together, Angptl4 modulates vascular permeability, angiogenesis, inflammatory signaling, and associated diseases. The use of Angptl4-modulating agents such as certain drugs, food constituents (such as fatty acids), nuclear factor (such as PPARα), and bacteria may treat associated diseases such as tumor metastasis, ischemic-reperfusion injury, inflammation, and chronic low-grade inflammation. However, the diverse physiological functions of Angptl4 in different tissues can lead to potentially deleterious side effects when used as a therapeutic target. In this regard, a better understanding of the underlying mechanisms for Angptl4 in different tissues is necessary. © 2013 Springer Basel. Source


Zhong Y.,No.324 Hospital of PLA | Chen Z.,General Hospital of Chengdu Military Region of PLA | Chen L.-B.,No.324 Hospital of PLA | Liao M.-S.,General Hospital of Chengdu Military Region of PLA | Deng D.,General Hospital of Chengdu Military Region of PLA
Chinese Journal of Medical Imaging Technology | Year: 2013

Objective: To investigate the effect of high mechanical index diagnostic ultrasound combined with microbubbles on the neovasculature of Walker-256 tumor in rats. Methods: Twenty-nine SD rats bearing subcutaneous Walker-256 tumor were randomly divided into 3 groups: Ultrasound+microbubbles group (n=15), ultrasound group (n=7) and the sham group (n=7). CEUS was performed to acquire the baseline tumor blood perfusion of all the tumors. During the procedure, the tumors were insonated with 20 times of continuous acoustic radiation force impulse imaging. At the same time of ARFI performance, 0.04 ml microbubbles was instantly injected into the tail vein. The ultrasound group and sham group were applied with only diagnostic ultrasound exposure or microbubbles injection, respectively. CEUS was repeated to observe the changes of perfusion before insonation and 0, 10 and 20 min after insonation. Three rabbits were harvested randomly in every group for histological examination immediately after insonation. Results: The mean peak intensity (PI) values of ROI displayed significant decreases from 25.17% to 12.01% in ultrasound+microbubbles group (P<0.01). In the ultrasound group and sham group, media was filling quickly and perfusion was good. In ultrasound+microbubbles group, the blood flow recovered gradually, but PI still reduced after 10 min, the blood flow of tumor recovered basically complete and showed high perfusion phase after 20 min (P>0.05). Conclusion: Microbubbles enhanced high mechanical index diagnostic ultrasound can temporally decrease the microcirculation of Walker-256 tumor in rats. Copyright © 2013 by the Press of Chinese Journal of Medical Imaging Technology. Source


Hao L.,No.324 Hospital of PLA | Zou Z.,Beijing Research Institute of Chemical Defense | Tian H.,No.324 Hospital of PLA | Zhang Y.,No.324 Hospital of PLA | And 2 more authors.
BioMed Research International | Year: 2014

In recent years, stem cell-based approaches have attracted more attention from scientists and clinicians due to their possible therapeutical effect on stroke. Animal studies have demonstrated that the beneficial effects of stem cells including embryonic stem cells (ESCs), inducible pluripotent stem cells (iPSCs), neural stem cells (NSCs), and mesenchymal stem cell (MSCs) might be due to cell replacement, neuroprotection, endogenous neurogenesis, angiogenesis, and modulation on inflammation and immune response. Although several clinical studies have shown the high efficiency and safety of stem cell in stroke management, mainly MSCs, some issues regarding to cell homing, survival, tracking, safety, and optimal cell transplantation protocol, such as cell dose and time window, should be addressed. Undoubtably, stem cell-based gene therapy represents a novel potential therapeutic strategy for stroke in future. © 2014 Lei Hao et al. Source


Cai Y.,No.324 Hospital of PLA | Wu X.,No.324 Hospital of PLA | Shi Y.,No.324 Hospital of PLA | Liu W.,No.324 Hospital of PLA
Open Life Sciences | Year: 2015

The aim of this work was to investigate the different evolutionary patterns between HBeAg(+) and HBeAg(-) status of genotype B and genotype C of Hepatitis B virus (HBV). Complete HBV genome sequences were obtained from GenBank, and were classified as HBeAg(+) of genotype B, HBeAg(-) of genotype B, HBeAg(+) of genotype C, and HBeAg(-) of genotype C. The results showed that genotype B had lower diversity and a longer phylogenetic tree than genotype C. This means that genotype B was more conserved than genotype C. The genetic distance of HBeAg(+) was shorter than that of HBeAg(-) in genotype B. dN, dS, dN/dS of HBeAg(+) were lower than that of HBeAg(-). In addition, the Core and S genes had lower diversity, and were under lower selective pressure than other genes. We propose that by having different HBeAg HBV receives disparate immune selective pressures, however these different genotypes also receive disparate natural selective pressures. The evolutionary pattern of HBV could be one of the important factors that may affect viral replication in HBV infected patients and could therefore play an important role in the diseases progression. © 2015 Ying Cai et al. 2015. Source

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