Liu Z.,No 324 Hospital Of Peoples Liberation Army |
Wang Z.,Chongqing Medical University |
Lu B.,Chongqing Medical University |
Hu C.,Chongqing Medical University |
And 4 more authors.
Chinese Journal of Cancer Biotherapy | Year: 2013
Objective:To explore the inhibitory effect of miRNA-29a(mi-29a) on the proliferation of human gastric cancer cell lines SGC-7901 and AGS through over-expression of miR-29a mediated by the recombinant replication-deficient human adenovirus type 5 vector. Methods: The recombinant adenovirus Ad-miR-29a containing pre-miR-29a or control adenovirus Ad-LacZ containing LacZ gene was constructed and infected into human gastric cancer SGC-7901 and AGS cells, respectively. The expressions of miR-29a in SGC-7901 and AGS cells were detected by real-time PCR. CCK-8 assay was employed to examine the inhibitory effect of miR-29a on the proliferation of human gastric cancer cell lines. The flow cytometry assay was used to analyze cell cycle of SGC-7901 and AGS cells. The migration ablilities of SGC-7901 and AGS cells were assessed by Transwell assay. Results: Compared with the Ad-LacZ group, the Ad-miR29a group expressed higher level of miR-29a in both SGC-7901 and AGS cells ([17.35 ± 0. 71] vs [1.12 ± 0.09], [26. 50 ± 1.09] vs [0. 95 ± 0. 04], P<0. 01). The proliferation of S-7901 and AGS was significantly inhibited in the Ad-miR29a group as compared with that in the Ad-LacZ group. Six days after Ad-miR29a infection, the D value was significantly decreased ([0.54 ± 0.03] vs [0.77 ± 0.04], [0.70 ± 0.03] vs [0.88 ± 0.04], P < 0. 01). The proliferation of cells in the Ad-LacZ group showed no significant changes as compared with that in the uninfected group (P > 0.05). Meanwhile, The percentage of SGC-7901 or AGS cells arrested in Go/Gj period in the Ad-miR29a group was significantly higher than that in the Ad-LacZ group ([63.10 ± 4.91]% vs [47.60 ± 5. 31]%,[69.80 ± 3.15]% vs [54.60 ±4.22 J%, P < 0.05 J. However, no significant changes were found in the migration ability of gastric cancer SGC-7901 and AGS cells. Conclusion: miR-29a can effectively suppress the proliferation of human gastric cancer cells, which makes a promising new therapeutic target for gastric cancer.
Hao L.,No 324 Hospital Of Peoples Liberation Army |
Guo X.,Chongqing Medical University |
Zou C.,No 324 Hospital Of Peoples Liberation Army |
Zhou H.,No 324 Hospital Of Peoples Liberation Army |
And 4 more authors.
Croatian Medical Journal | Year: 2016
Aim To explore the effects of hyperbaric oxygen preconditioning (HBOP) on the permeability of blood-brain barrier (BBB) and expression of tight junction proteins under hypoxic conditions in vitro. Methods A BBB in vitro model was constructed using the hCMEC/D3 cell line and used when its trans-endothelial electrical resistance (TEER) reached 80-120 Ω· cm2 (tested by Millicell-Electrical Resistance System). The cells were randomly divided into the control group cultured under normal conditions, the group cultured under hypoxic conditions (2%O2) for 24 h (hypoxia group), and the group first subjected to HBOP for 2 h and then to hypoxia (HBOP group). Occludin and ZO-1 expression were analyzed by immunofluorescence assay. Results Normal hCMEC/D3 was spindle-shaped and tightly integrated. TEER was significantly reduced in the hypoxia (P = 0.001) and HBOP group (P = 0.014) compared to control group, with a greater decrease in the hypoxia group. Occludin membranous expression was significantly decreased in the hypoxia group (P = 0.001) compared to the control group, but there was no change in the HBOP group. ZO-1 membranous expression was significantly decreased (P = 0.002) and cytoplasmic expression was significantly increased (P = 0.001) in the hypoxia group compared to the control group, although overall expression levels did not change. In the HBOP group, there was no significant change in ZO-1 expression compared to the control group. Conclusion Hyperbaric oxygen preconditioning protected the integrity of BBB in an in vitro model through modulation of occludin and ZO-1 expression under hypoxic conditions.
PubMed | No 324 Hospital Of Peoples Liberation Army
Type: Journal Article | Journal: Zhonghua wai ke za zhi [Chinese journal of surgery] | Year: 2010
To explore and develop three-dimension (3D) virtual reality (VR) liver model and convert computed tomography data into a fully 3D VR environment for display, measure and manipulation.3D-reconstruction of liver was restored from spiral computed tomography (CT) data by using LiVirtue software. Dextrobeam was used to view the 3D model in the VR environment. The liver and its anatomic structure were reconstructed to illuminate the location of the tumor and its related vessels.3D models of liver, tumor and their relative vessels were reconstructed successfully. These models could be viewed and manipulated in the VR environment on personal computer.38 patients underwent liver resection, including 21 right hemihepatectomy, 14 left hemihepatectomy and 3 extended right hemihepatectomy. The intraoperative contrast with preoperative simulation confirmed the reliability of our 3D operative planning system.The preoperative simulation in 3D VR facilitated liver resection by the ability to view tumor and its relative vessels. This preoperative estimation from 3D model of liver benefits a lot to complicated liver resection.