Liao S.,No. 181 Hospital of PLA |
Ma Q.,Institute of Health and Environmental Medicine |
An G.,Institute of Health and Environmental Medicine |
Zhang N.,Institute of Health and Environmental Medicine |
And 7 more authors.
Digest Journal of Nanomaterials and Biostructures | Year: 2013
Solid dispersions of Asiatic acid with a hydrophilic polymer, namely, polyvinyl pyrrolidone (PVP) was prepared by the solvent evaporation method. The effect of Asiatic acid: PVPk30 feed ratio by weight on the aqueous solubility was investigated, the aqueous solubility of Asiatic acid reached 2043μg/ml when the weight ratio of Asiatic acid to PVPk30 was 1:5. The aqueous solubility of Asiatic acid was increased by 20-fold in Asiatic acid/ PVPk30 solid dispersions. The Asiatic acid/ PVPk30 solid dispersions system was characterized by Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC) and Scanning Electron Microscopy (SEM). The FTIR spectra of Asiatic acid/ PVPk30 solid dispersions showed that the presence of strong interactions between Asiatic acid and PVPk30. The SEM and DSC spectrum of Asiatic acid/ PVPk30 solid dispersions indicated Asiatic acid existed in amorphous state, this could be explained the fact that the aqueous solubility of Asiatic acid was increased.
Zhang J.,No. 303 Hospital of PLA |
Li S.,No. 303 Hospital of PLA |
Leng J.,No. 303 Hospital of PLA |
Chen Y.,No. 181 Hospital of PLA |
And 9 more authors.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2013
OBJECTIVE: To reveal the relationship between iodine nutrition and the change of spectrum on thyroid diseases through comparing the different iodine environments pre- and post- the universal salt iodization(USI)campaign.METHODS: To compare the urinary iodine concentration between 1000 normal people and 5998 patients with thyroid disease who had undergone surgical operations, from 4 major cities, including iodine deficient and rich areas of Guangxi Zhuang Autonomous Region.RESULTS: After USI was put into practice, the urinary iodine concentration of patients with thyroid appeared higher than those of normal people(324.3 µg/L vs. 238.5 µg/L, P < 0.05). The urinary iodine concentrations of nodular goiter,Graves disease, toxic nodular goiter, thyroid papillary carcinoma and Hashimoto's thyroiditis were higher than those before the USI was taken(263.8 µg/L vs. 69.75 µg/L, 289.7 µg/L vs. 228.3 µg/L, 346.8 µg/L vs. 268.4 µg/L, 350.3 µg/L vs. 316.2 µg/L and 378.5 µg/L vs. 305.8 µg/L). The proportions of toxic nodular goiter, thyroid papillary carcinoma and Hashimoto's thyroiditis appeared as 7.59% vs. 4.80%, 5.85% vs. 4.02% and 3.88% vs. 2.46%, all higher than those before the implementation of USI, except the nodular goiter which showed a reduction (63.56% vs. 69.75%).CONCLUSION: The spectrum of thyroid diseases appeared an obvious change in Guangxi within the last 10-year implementation of USI. However, the excessive intake of iodine might serve as a risk factor for toxic nodular goiter, thyroid papillary carcinoma and Hashimoto's thyroiditis.
Li P.,Chongqing Medical University |
Shi R.,Kunming General Hospital of Chengdu Command |
Chen D.,Chinese Peoples Liberation Army |
Gan Y.,Chongqing Medical University |
And 4 more authors.
BMC Musculoskeletal Disorders | Year: 2016
Background: The maintenance of nucleus pulposus (NP) viability in vitro is difficult. The annulus fibrosus (AF) pathway reflects one nutrient transport channel and may have an important effect on NP viability in disc organ cultures. The present study describes a feasible disc pre-treatment involving the AF and investigates its efficacy in improving NP bioactivity in an in vitro disc bioreactor culture. Methods: Rabbit discs that were randomly assigned to the experimental group (EG) were pretreated via the surgical removal and controlled trypsinization of the outer AF. The discs in the control group (CG) did not receive any special treatment. All discs were organ-cultured in a self-developed bioreactor. Solute transport into the central NP was measured using a methylene blue solution. On days 7 and 14, histological properties, cell viability, cell membrane damage, gene expression and matrix composition within the NP in these two groups were compared with each other and with the corresponding parameters of fresh NP samples. Additionally, the structures of the outer AF and the cartilage endplate (CEP) following pre-treatment were also assessed. Results: The outer AF in the EG became disorganized, but no specific changes occurred in the CEP or the inner AF following pre-treatment. The discs in the EG exhibited increased penetration of methylene blue into the central NP. On days 7 and 14, the NP bioactivity in the EG was improved compared with that of the CG in terms of cell viability, cell membrane damage, gene expression profile and matrix synthesis. Moreover, cell viability and matrix synthesis parameters in the EG were more similar to those of fresh samples than they were to the same parameters in the CG on day 14. Conclusions: Using this disc pre-treatment, i.e., the surgical removal and controlled trypsinization of the outer AF, NP bioactivity was better maintained for up to 14 days in an in vitro disc bioreactor culture. © 2016 Li et al.
Qu L.,No. 181 Hospital of PLA |
Li L.,No. 181 Hospital of PLA
Chinese Ophthalmic Research | Year: 2010
Fungal keratitis is the first common infectious corneal disease and has become the main cause of blindness in China. Along with the intensive explore for the pathogenic mechanisms, the significant progress has been achieved in the pharmacotherapeutics. For lowering its blindnessrate, it is imperative to the study on increasing therapeutic option and enhancing therapeutic efficacy. This review summarizes the current advance in clinical use in ophthalmology of novel antifungal agents, including new azoles such as voriconazole and posaconazole, and echinocandins such as caspofungin and micafungin. This article also reviews the progress of administration routes for the management of recalcitrant fungal keratitis, including subconjunctival, interstromal, intracameral and intravitreal injection.
Li L.-M.,No. 181 Hospital of PLA |
Zhao L.-Q.,No. 181 Hospital of PLA |
Qu L.-H.,No. 181 Hospital of PLA |
Li P.,No. 181 Hospital of PLA
Journal of Ophthalmology | Year: 2014
This retrospective study was to evaluate treatment outcomes of excimer laser phototherapeutic keratectomy (PTK) for clinically presumed fungal keratitis. Forty-seven eyes of 47 consecutive patients underwent manual superficial debridement and PTK. All corneal lesions were located in the anterior stroma and were resistant to medication therapy for at least one week. Data were collected by a retrospective chart review with at least six months of follow-up data available. After PTK, infected corneal lesions were completely removed and the clinical symptoms resolved in 41 cases (87.2%). The mean ablation depth was 114.39±45.51 m and diameter of ablation was 4.06±1.07 mm. The mean time for healing of the epithelial defect was 8.8±5.6 days. Thirty-four eyes (82.9%) showed an improvement in best spectacle-corrected visual acuity of two or more lines. PTK complications included mild to moderate corneal haze, hyperopic shift, irregular astigmatism, and thinning cornea. Six eyes (12.8%) still showed progressed infection, and conjunctival flap covering, amniotic membrane transplantation, or penetrating keratoplasty were given. PTK is a valuable therapeutic alternative for superficial infectious keratitis. It can effectively eradicate lesions, hasten reepithelialization, and restore and preserve useful visual function. However, the selection of surgery candidates should be conducted carefully. © 2014 Liang-Mao Li et al.