Guilin, China
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Li X.,No 181 Hospital Of Pla | Li X.,Urologic | Liao S.,No 181 Hospital Of Pla | Yu Y.,No 181 Hospital Of Pla | And 4 more authors.
Urological Research | Year: 2012

The objective of the study was to establish and evaluate a modiWed puncture and dilation technique- "stereotaxic localisation" system-and the corresponding instruments for percutaneous nephrostomy. Four hundred patients were randomised to the intervention group (200 cases, stereotaxic location puncture and dilation procedures) and the traditional group (200 cases, traditional procedure) under X-ray guidance. In the modiWed intervention system, the distance and horizontal angle of the puncture pathway between the puncture point and the target site were calculated accurately. The time for punctures, time with X-ray exposure and operation, success rate of each puncture to access the target, number of patients requiring blood transfusion, stone clearance, drops of haemoglobin and days of hospital stay were compared between the two groups. In the traditional and intervention groups, the time for puncture was 17 and 7 min, respectively; the X-ray exposure time was 9.1 and 1.3 min; the cases requiring blood transfusion were 9 and 5; and the success rates for each puncture to access the target were 42.9 and 88.0%, all with statistical diVerence (p < 0.01). The other outcomes and complications revealed similar trends. The stereotaxic localisation system for puncture is statistically better than the traditional procedure used in this study. The stereotaxic localisation system in this study is safer and more accurate, and provides easier access to the target with less bleeding and reduced exposure to X-ray compared to the traditional puncture and dilation procedures of percutaneous nephrostomy. © Springer-Verlag 2011.


Yan Q.,No 181 Hospital Of Pla | Sui W.,No 181 Hospital Of Pla | Xie S.,No 181 Hospital Of Pla | Chen H.,No 181 Hospital Of Pla | And 3 more authors.
Transplant Immunology | Year: 2010

Objective: To investigate the expression of integrin-linked kinase (ILK) and collagen IV in renal allografts with interstitial fibrosis and tubular atrophy (IF/TA) in kidney transplant recipients, and explore its relationship with transforming growth factor-β1 (TGF-β1) expression and the pathogenesis of IF/TA. Methods: Immunohistochemical assay and computer-assisted genuine colored image analysis system were used to detect the expression of ILK, TGF-β1 and collagen IV in the renal allografts with IF/TA. The association between TGF-β1, collagen IV and ILK, as well as the relationship between their expressions and the pathological class of IF/TA, was analyzed. 10 specimens of healthy renal tissue were used as controls. Results: The expression levels of ILK, TGF-β1 and collagen IV in renal allografts were significantly higher, compared to normal renal tissues (P<0.001), and the expressions tended to increase along with the increase of pathological class of IF/TA. In IF/TA group, the expression of ILK was positively correlated with the expression of TGF-β1 and collagen IV (r=0.976 and r=0.912, respectively; P<0.001 for both). Conclusion: It is suggested that by the data that ILK might mediate the mechanism through which TGF-β1 promote the abnormal deposition of ECM in renal allografts with IF/TA. ILK might play an important role in the progression of the interstitial fibrosis and tubular atrophy of human renal allografts and the development of chronic renal allograft dysfunction. © 2010 Elsevier B.V.


PubMed | Hospital of Guangxi Youjiang Medical College, No 181 Hospital Of Pla, Peoples Hospital of Beihai City of Guangxi and No 303 Hospital Of Pla
Type: Journal Article | Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2013

To reveal the relationship between iodine nutrition and the change of spectrum on thyroid diseases through comparing the different iodine environments pre- and post- the universal salt iodization(USI)campaign.To compare the urinary iodine concentration between 1000 normal people and 5998 patients with thyroid disease who had undergone surgical operations, from 4 major cities, including iodine deficient and rich areas of Guangxi Zhuang Autonomous Region.After USI was put into practice, the urinary iodine concentration of patients with thyroid appeared higher than those of normal people(324.3 g/L vs. 238.5 g/L, P < 0.05). The urinary iodine concentrations of nodular goiter,Graves disease, toxic nodular goiter, thyroid papillary carcinoma and Hashimotos thyroiditis were higher than those before the USI was taken(263.8 g/L vs. 69.75 g/L, 289.7 g/L vs. 228.3 g/L, 346.8 g/L vs. 268.4 g/L, 350.3 g/L vs. 316.2 g/L and 378.5 g/L vs. 305.8 g/L). The proportions of toxic nodular goiter, thyroid papillary carcinoma and Hashimotos thyroiditis appeared as 7.59% vs. 4.80%, 5.85% vs. 4.02% and 3.88% vs. 2.46%, all higher than those before the implementation of USI, except the nodular goiter which showed a reduction (63.56% vs. 69.75%).The spectrum of thyroid diseases appeared an obvious change in Guangxi within the last 10-year implementation of USI. However, the excessive intake of iodine might serve as a risk factor for toxic nodular goiter, thyroid papillary carcinoma and Hashimotos thyroiditis.


Li P.,Chongqing Medical University | Shi R.,Kunming General Hospital of Chengdu Command | Chen D.,Chinese People's Liberation Army | Gan Y.,Chongqing Medical University | And 4 more authors.
BMC Musculoskeletal Disorders | Year: 2016

Background: The maintenance of nucleus pulposus (NP) viability in vitro is difficult. The annulus fibrosus (AF) pathway reflects one nutrient transport channel and may have an important effect on NP viability in disc organ cultures. The present study describes a feasible disc pre-treatment involving the AF and investigates its efficacy in improving NP bioactivity in an in vitro disc bioreactor culture. Methods: Rabbit discs that were randomly assigned to the experimental group (EG) were pretreated via the surgical removal and controlled trypsinization of the outer AF. The discs in the control group (CG) did not receive any special treatment. All discs were organ-cultured in a self-developed bioreactor. Solute transport into the central NP was measured using a methylene blue solution. On days 7 and 14, histological properties, cell viability, cell membrane damage, gene expression and matrix composition within the NP in these two groups were compared with each other and with the corresponding parameters of fresh NP samples. Additionally, the structures of the outer AF and the cartilage endplate (CEP) following pre-treatment were also assessed. Results: The outer AF in the EG became disorganized, but no specific changes occurred in the CEP or the inner AF following pre-treatment. The discs in the EG exhibited increased penetration of methylene blue into the central NP. On days 7 and 14, the NP bioactivity in the EG was improved compared with that of the CG in terms of cell viability, cell membrane damage, gene expression profile and matrix synthesis. Moreover, cell viability and matrix synthesis parameters in the EG were more similar to those of fresh samples than they were to the same parameters in the CG on day 14. Conclusions: Using this disc pre-treatment, i.e., the surgical removal and controlled trypsinization of the outer AF, NP bioactivity was better maintained for up to 14 days in an in vitro disc bioreactor culture. © 2016 Li et al.


PubMed | No 181 Hospital Of Pla, Chongqing Medical University and Chongqing Three Gorges Central Hospital
Type: Journal Article | Journal: International journal of medical sciences | Year: 2016

Previous cell culture and animal in vivo studies indicate the obvious effects of mechanical compression on disc cell biology. However, the effects of dynamic compression magnitude, frequency and duration on the immature nucleus pulposus (NP) from an organ-cultured disc are not well understood.To investigate the effects of a relatively wide range of compressive magnitudes, frequencies and durations on cell apoptosis and matrix composition within the immature NP using an intelligent and mechanically active bioreactor.Discs from the immature porcine were cultured in a mechanically active bioreactor for 7 days. The discs in various compressive magnitude groups (0.1, 0.2, 0.4, 0.8 and 1.3 MPa at a frequency of 1.0 Hz for 2 hours), frequency groups (0.1, 0.5, 1.0, 3.0 and 5.0 Hz at a magnitude of 0.4 MPa for 2 hours) and duration groups (1, 2, 4 and 8 hours at a magnitude of 0.4 MPa and frequency of 1.0 Hz) experienced dynamic compression once per day. Discs cultured without compression were used as controls. Immature NP samples were analyzed using the TUNEL assay, histological staining, glycosaminoglycan (GAG) content measurement, real-time PCR and collagen II immunohistochemical staining.In the 1.3 MPa, 5.0 Hz and 8 hour groups, the immature NP showed a significantly increase in apoptotic cells, a catabolic gene expression profile with down-regulated matrix molecules and up-regulated matrix degradation enzymes, and decreased GAG content and collagen II deposition. In the other compressive magnitude, frequency and duration groups, the immature NP showed a healthier status regarding NP cell apoptosis, gene expression profile and matrix production.Cell apoptosis and matrix composition within the immature NP were compressive magnitude-, frequency- and duration-dependent. The relatively high compressive magnitude or frequency and long compressive duration are not helpful for maintaining the healthy status of an immature NP.


PubMed | Kunming General Hospital of Chengdu Command, Chongqing Medical University, Chinese People's Liberation Army and No 181 Hospital Of Pla
Type: | Journal: BMC musculoskeletal disorders | Year: 2016

The maintenance of nucleus pulposus (NP) viability in vitro is difficult. The annulus fibrosus (AF) pathway reflects one nutrient transport channel and may have an important effect on NP viability in disc organ cultures. The present study describes a feasible disc pre-treatment involving the AF and investigates its efficacy in improving NP bioactivity in an in vitro disc bioreactor culture.Rabbit discs that were randomly assigned to the experimental group (EG) were pretreated via the surgical removal and controlled trypsinization of the outer AF. The discs in the control group (CG) did not receive any special treatment. All discs were organ-cultured in a self-developed bioreactor. Solute transport into the central NP was measured using a methylene blue solution. On days 7 and 14, histological properties, cell viability, cell membrane damage, gene expression and matrix composition within the NP in these two groups were compared with each other and with the corresponding parameters of fresh NP samples. Additionally, the structures of the outer AF and the cartilage endplate (CEP) following pre-treatment were also assessed.The outer AF in the EG became disorganized, but no specific changes occurred in the CEP or the inner AF following pre-treatment. The discs in the EG exhibited increased penetration of methylene blue into the central NP. On days 7 and 14, the NP bioactivity in the EG was improved compared with that of the CG in terms of cell viability, cell membrane damage, gene expression profile and matrix synthesis. Moreover, cell viability and matrix synthesis parameters in the EG were more similar to those of fresh samples than they were to the same parameters in the CG on day 14.Using this disc pre-treatment, i.e., the surgical removal and controlled trypsinization of the outer AF, NP bioactivity was better maintained for up to 14days in an in vitro disc bioreactor culture.


Qu L.,No 181 Hospital Of Pla | Li L.,No 181 Hospital Of Pla
Chinese Ophthalmic Research | Year: 2010

Fungal keratitis is the first common infectious corneal disease and has become the main cause of blindness in China. Along with the intensive explore for the pathogenic mechanisms, the significant progress has been achieved in the pharmacotherapeutics. For lowering its blindnessrate, it is imperative to the study on increasing therapeutic option and enhancing therapeutic efficacy. This review summarizes the current advance in clinical use in ophthalmology of novel antifungal agents, including new azoles such as voriconazole and posaconazole, and echinocandins such as caspofungin and micafungin. This article also reviews the progress of administration routes for the management of recalcitrant fungal keratitis, including subconjunctival, interstromal, intracameral and intravitreal injection.


Li L.-M.,No 181 Hospital Of Pla | Zhao L.-Q.,No 181 Hospital Of Pla | Qu L.-H.,No 181 Hospital Of Pla | Li P.,No 181 Hospital Of Pla
Journal of Ophthalmology | Year: 2014

This retrospective study was to evaluate treatment outcomes of excimer laser phototherapeutic keratectomy (PTK) for clinically presumed fungal keratitis. Forty-seven eyes of 47 consecutive patients underwent manual superficial debridement and PTK. All corneal lesions were located in the anterior stroma and were resistant to medication therapy for at least one week. Data were collected by a retrospective chart review with at least six months of follow-up data available. After PTK, infected corneal lesions were completely removed and the clinical symptoms resolved in 41 cases (87.2%). The mean ablation depth was 114.39±45.51 m and diameter of ablation was 4.06±1.07 mm. The mean time for healing of the epithelial defect was 8.8±5.6 days. Thirty-four eyes (82.9%) showed an improvement in best spectacle-corrected visual acuity of two or more lines. PTK complications included mild to moderate corneal haze, hyperopic shift, irregular astigmatism, and thinning cornea. Six eyes (12.8%) still showed progressed infection, and conjunctival flap covering, amniotic membrane transplantation, or penetrating keratoplasty were given. PTK is a valuable therapeutic alternative for superficial infectious keratitis. It can effectively eradicate lesions, hasten reepithelialization, and restore and preserve useful visual function. However, the selection of surgery candidates should be conducted carefully. © 2014 Liang-Mao Li et al.


PubMed | No 181 Hospital Of Pla
Type: Journal Article | Journal: Urological research | Year: 2012

The objective of the study was to establish and evaluate a modified puncture and dilation technique-stereotaxic localisation system-and the corresponding instruments for percutaneous nephrostomy. Four hundred patients were randomised to the intervention group (200 cases, stereotaxic location puncture and dilation procedures) and the traditional group (200 cases, traditional procedure) under X-ray guidance. In the modified intervention system, the distance and horizontal angle of the puncture pathway between the puncture point and the target site were calculated accurately. The time for punctures, time with X-ray exposure and operation, success rate of each puncture to access the target, number of patients requiring blood transfusion, stone clearance, drops of haemoglobin and days of hospital stay were compared between the two groups. In the traditional and intervention groups, the time for puncture was 17 and 7 min, respectively; the X-ray exposure time was 9.1 and 1.3 min; the cases requiring blood transfusion were 9 and 5; and the success rates for each puncture to access the target were 42.9 and 88.0%, all with statistical difference (p < 0.01). The other outcomes and complications revealed similar trends. The stereotaxic localisation system for puncture is statistically better than the traditional procedure used in this study. The stereotaxic localisation system in this study is safer and more accurate, and provides easier access to the target with less bleeding and reduced exposure to X-ray compared to the traditional puncture and dilation procedures of percutaneous nephrostomy.


PubMed | No 181 Hospital of PLA
Type: Journal Article | Journal: Transplant immunology | Year: 2010

To investigate the expression of integrin-linked kinase (ILK) and collagen IV in renal allografts with interstitial fibrosis and tubular atrophy (IF/TA) in kidney transplant recipients, and explore its relationship with transforming growth factor-beta(1) (TGF-beta(1)) expression and the pathogenesis of IF/TA.Immunohistochemical assay and computer-assisted genuine colored image analysis system were used to detect the expression of ILK, TGF-beta(1) and collagen IV in the renal allografts with IF/TA. The association between TGF-beta(1), collagen IV and ILK, as well as the relationship between their expressions and the pathological class of IF/TA, was analyzed. 10 specimens of healthy renal tissue were used as controls.The expression levels of ILK, TGF-beta(1) and collagen IV in renal allografts were significantly higher, compared to normal renal tissues (P<0.001), and the expressions tended to increase along with the increase of pathological class of IF/TA. In IF/TA group, the expression of ILK was positively correlated with the expression of TGF-beta(1) and collagen IV (r=0.976 and r=0.912, respectively; P<0.001 for both).It is suggested that by the data that ILK might mediate the mechanism through which TGF-beta(1) promote the abnormal deposition of ECM in renal allografts with IF/TA. ILK might play an important role in the progression of the interstitial fibrosis and tubular atrophy of human renal allografts and the development of chronic renal allograft dysfunction.

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