No 174 Hospital Of Pla

Xiamen, China

No 174 Hospital Of Pla

Xiamen, China
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Wen X.,No 174 Hospital Of Pla | Wen X.,Shanghai University | Li H.,Chongqing Medical University | Li Y.,Jiamusi University | Zhang W.,Shanghai University
Pharmaceutical Care and Research | Year: 2010

Objective: To study the effect of baicalin on physiological functions of subacute ageing mice induced by D-galactose. Methods: Mice were randomly divided into 7 groups, including control group, model group, lentinan(200 mg/kg) group, baicalin low, middle and high dose (50,100,200 mg/kg) groups, baicalin(100 mg/kg)+ lentinan( 100 mg/kg) group. After gavage administration of drugs for 24 days, the learning and memory ability of mice were tested. Simultaneously the activities of superoxide dismutase(SOD), glutathione peroxidase (GSH-Px), catalase(CAT), and in vivo content of malonaldehyde (MDA) in mice were determined. Results: High- and middle-dose baicalin(P<0.05) could improve the abilities of learning and memory of mice, increase the levels of SOD, GSH-Px and CTA, reduce the content of MDA, but the effects were weaker than lentinan. The effect of combination of baicalin and lentinan was the best. Conclusion: Baicalin could delay the ageing process of subacute ageing mice induced by D-galactose. The mechanism may be related to regulating the oxidative metabolism enzyme system. It may be used as an adjuvant anti-ageing drug in clinic.

Fan M.,Shanghai University | Shen J.,Shanghai University | Liu H.,Shanghai University | Wen Z.,No 174 Hospital Of Pla | And 6 more authors.
Oncology Reports | Year: 2017

Paired-related homeobox 1 (PRRX1) has been identified as a novel molecule associated with induction of epithelial-mesenchymal transition (EMT), acquisition of cancer stem cell like properties and poor prognosis in tumors. However, the function of PRRX1 in hepatocellular carcinoma has not been elucidated. In the present study, we observed that PRRX1 expression levels were downregulated and positively correlated with the downregulated expression of p53 in hepatocellular carcinoma specimens. Decreased expression of PRRX1 and/or p53 by siRNA induced both the migration and the invasion features of HCC cells in vitro. Furthermore, the loss of PRRX1 inhibits hepatocellular carcinoma (HCC) cell apoptosis, an anti-apoptotic expression profile was upregulated accompanied by downregulated expression of p53. HCC patients with low-expression of both PRRX1 and p53 had a significantly shorter overall and disease-free survival. These findings demonstrate that PRRX1 plays an important role in metastasis and apoptosis of HCC cells through the p53-dependent signaling pathway and is expected to become a novel biomarker associated with patient prognosis and survival.

Ding Y.,No 174 Hospital Of Pla | Chen Y.,No 174 Hospital Of Pla | Li Q.,No 174 Hospital Of Pla | Mi Y.J.,No 174 Hospital Of Pla | And 2 more authors.
Chinese Journal of Cancer Biotherapy | Year: 2015

Objective: To evaluate the effect of Helicobacter pylori (Hp) infection on the outcome of maintenance therapy with autologous dendritic cells-cytokine induced killer (DC-CIK) cells in gastric cancer patients. Methods: Seventy-two patients with advanced gastric cancer, aged 29-90 years, who were admitted to the Department of Oncology of No. 174 Hospital of PLA between June, 2010 and June, 2012 were included in this study. Based on the gastroscopic findings, patients were divided into two groups: Hp-positive and Hp-negative. Both groups received two courses of autologous DC-CIK immunotherapy after surgery or radiotherapy and chemotherapy. At the end of treatment, DC differentiation and maturation, treatment efficacy and patients outcomes in the two groups were analyzed. Results: No significant difference was observed in the morphology and maturation status of DCs between two groups (P>0.05). Levels of CD83 and CD86 on the surface of DCs were significantly higher in the Hp-positive group than those in the Hp-negative group (P<0.01), but surface levels of HLA-DR in DCs had no significant difference between two groups (P>0.05). In the Hp-positive group, the quality of life KPS score and levels of T lymphocyte markers (CD3+, CD4+ and CD8+) were significantly increased (P<0.05) but levels of tumor markers CEA, CA199 and CA724 were significantly decreased (P<0.05) after treatment. In the Hp-negative group, levels of tumor markers and the T lymphocyte markers were significantly different before and after treatment (P<0.05), but the KPS score was not significantly improved (P>0.05). Hp-positive patients had better KPS score, higher levels of CEA and CA199, and more abundant CD3+ CD4+T cells than Hp-negative patients (P<0.05). In contrast, the two groups did not significantly differ in tumor stability, CA724 expression, and the number of CD8+T cells (P>0.05). At the end of 2-years follow-up, the median survival time was 12.64 months in the HP-positive group and 11.42 months in the HP-negative group (P<0.05). Conclusion: Hp infection may help stabilize the tumor size, improve the quality of life, and prolong the survival time in patients with advanced gastric cancer undergoing maintenance therapy with DC-CIK cells. © 2015, Editorial office of Chinese Journal of Cancer Biotherapy. All rights reserved.

Li Q.,No 174 Hospital Of Pla | Chen Y.,No 174 Hospital Of Pla | Yan J.,Xiamen University | Ding Y.,No 174 Hospital Of Pla | And 5 more authors.
Chinese Journal of Clinical Oncology | Year: 2013

Objective: This study aimed to compare and analyze the functional differences between peripheral blood monocyte-derived dendritic cells (DCs) of Helicobacter pylori-positive and H. pylori-negative patients with gastric cancer. Methods: H. pylori infection was detected in 84 patients with gastric cancer in our hospital from January 2011 to October 2012 by the 14C-urea breath test. DCs were generated from monocytes isolated by an adherent method from the two groups of patients and cultured in the presence of rhIL-4, rhGM-CSF, and rhTNF-α. Furthermore, the expression of surface marker molecules was determined by fluorescence-activated cell sorting analysis. The cytotoxicity of DCs pulsed T cells against gastric carcinoma cell was assessed by the lactate dehydrogenase-releasing assay. The secretion of IL-12 and IFN-γ in the supernatant was determined by enzyme-linked immunosorbent assay. Results: No difference was observed in the morphological change of the maturation process. The mean expression of CD1a, CD80, CD83, CD86, and HLA-DR molecules in DCs of H. pylori-infected patients was higher than that in DCs of H. pylori-negative group, and the differences were statistically significant except for CD1a and HLA-DR. The cytotoxicity activities, IL-12 release, and IFN-γ release in the H. pylori-positive group were significantly higher than those in the H. pylori-negative group (P<0.05). Conclusion: H. pylori infection has no effect on the morphological change of the maturation process of monocyte-derived DCs. These data clearly demonstrate that monocyte-derived DCs of H. pylori-infected patients with gastric cancer can induce stronger maturation and activation than those of H. pylori-negative patients.

Wang L.,No 174 Hospital Of Pla | Qiu H.,No 174 Hospital Of Pla
Medical Journal of Wuhan University | Year: 2011

Objective: To evaluate the relationship between serum CA125 or EmAb levels and endometriosis in different locations. Methods: Ninety-six patients who had been diagnosed by laparoscopy or laparotomy with endometriosis and forty control women without endometriosis were included in this study. Three groups were divided according to the different locations of endometriosis as: Group A, ovarian endometriosis, 42 cases; Group B, pelvic endometriosis, 40 cases; Group C, scar endometriosis, 14 cases. Group D was the control group. The serum CA125 levels and EmAb assay were measured by enzyme-linked immunosorbent assay. Results: The serum CA125 levels in ovarian endometriosis were higher than those in control women (P < 0.05). The serum CA125 levels in pelvic endometriosis and scar endometriosis were lower than those in ovarian endometriosis (P < 0.05). The positive rate (sensitivity) of serum CA125 and EmAb was 69.04% and 45.23% respectively in ovarian endometriosis, 45.00% and 32.50% respectively in pelvic endometriosis, 42.85% and 28.57% respectively in scar endometriosis. And the specificity for CA125 and EmAb was 95% and 97.5% respectively. When both CA125 and EmAb assay were positive, the specificity was 100%. Conclusion: The serum CA125 and EmAb testing for the diagnosis of endometriosis has considerable value. Combined determination of serum CA125 and EmAb can achieve good specificity for the diagnosis of endometriosis.

Guo Z.-Q.,Anhui Medical University | Guo Z.-Q.,No 174 Hospital Of Pla | Qiu L.,Anhui Medical University | Gao Y.,No 174 Hospital Of Pla | And 5 more authors.
Burns | Year: 2016

Objective Extensive deep partial-thickness burns still seriously challenge the surgeon's abilities. This study aimed to assess the impact of early dermabrasion combined with porcine acellular dermal matrix (ADM) in extensive deep dermal burns. Methods From September 2009 to September 2013, a total of 60 adult patients sustained greater than 50% total body surface area (TBSA) burn by hot water or gas explosion were divided into three groups based on dermabrasion: group A (early dermabrasion and porcine ADM), group B (early dermabrasion and nano-silver dressings), and group C (conservative group). The wound healing time and length of hospital stay were analyzed. Scar assessment was performed at 3 and 12 months after the injury with a modified Vancouver Scar Scale linked with TBSA (mVSS-TBSA). Results No significant difference was found in mean burn size, burn depth, age, male-to-female ratio, or incidence of inhalation injury between the patients in the three groups (p > 0.05). Compared with groups B and C, the patients that received early dermabrasion combined with porcine ADM had a shorter wound healing time (p < 0.01). The burn patients treated with early dermabrasion and porcine ADM coverage had a mean length of hospital stay of 28.3 days (±7.2), which was significantly shorter than that of groups B and C (p < 0.05-0.01). The mVSS-TBSA of patients in group A was significantly improved in comparison with groups B and C at 3 and 12 months after the injury. There was no significant difference in the mortality rate between the three groups (p > 0.05). Conclusion Early dermabrasion combined with porcine ADM coverage facilitates wound healing, reduces the length of hospital stay, and improves esthetic and functional results in extensive deep dermal burns with burn size over 50% TBSA. © 2015 Elsevier Ltd and ISBI. All rights reserved.

Qiu H.,No 174 Hospital Of Pla | Wang L.,No 174 Hospital Of Pla | Sha A.,No 174 Hospital Of Pla
Medical Journal of Wuhan University | Year: 2010

Objective: To study the relationship of multiple human papillomavirus (HPV) infections in cervical lesion. Methods; The HPV-DNA typing test was performed in 3155 women, of which 537 positive cases underwent colposcopy and pathological examination of cervical biopsy. Results; HPV-DNA typing test showed single HPV infection was 12.80%, and multiple HPV infection was 4.20%. The most common model was double infection. The multiple infection rates increased with the severity of cervical dysplasia (except for cervicitis). Conclusion; Multiple HPV infection is associated with the development of cervical neoplasia. HPV infections testing in etiological diagnosis of cervical diseases should be applicated widely.

Jiang G.,Anhui University | Hu Y.,Anhui Medical University | Hu Y.,No 105 Hospital Of Pla | Liu L.,No 174 Hospital Of Pla | And 4 more authors.
Neurochemistry International | Year: 2014

Although the etiology of PD remains unclear, increasing evidence has shown that oxidative stress plays an important role in its pathogenesis and that of other neurodegenerative disorders. The phenolic glucoside gastrodin, a main constituent of a Chinese herbal medicine Gastrodia elata (GE) Blume, has been known to display antioxidant activity. The present study aimed to investigate the protective effects of gastrodin on 1-methyl-4-phenylpyridinium (MPP +)-induced oxidative cytotoxicity in human dopaminergic SH-SY5Y cells and the underlying mechanism for this neuroprotection. Results indicate that pre-treatment with gastrodin for 1 h significantly reduced the MPP +-induced viability loss, apoptotic rate and attenuated MPP +-mediated ROS production. In addition, gastrodin inhibited MPP +-induced lowered membrane potential, decreased Bcl-2/Bax ratio. Moreover, we have revealed the gastrodin increased Nrf2 nuclear translocation, which is upstream of heme oxygenase-1 (HO-1) expression and for the first time revealed gastrodin could increased antioxidant enzyme HO-1 expression in concentration-dependent and time-dependent manners. HO-1 siRNA transfection was employed, and confirmed gastrodin could active the expression of HO-1. And the increase in HO-1 expression was correlated with the protective effect of gastrodin against MPP+-induced injury. Because the inhibitor of HO-1 activity, ZnPP reversed the protective effect of gastrodin against MPP +-induced cell death. We also demonstrated that the specific p38 MAPK inhibitor, SB203580, concentration-dependently blocked on gastrodin-induced HO-1 expression, and meanwhile SB203580 reversed the protective effect of gastrodin against MPP+-induced cell death. Taken together, these findings suggest that gastrodin can induce HO-1 expression through activation of p38 MAPK/Nrf2 signaling pathway, thereby protecting the SH-SY5Y cells from MPP+-induced oxidative cell death. Thus our study indicates that gastrodin has a partial cytoprotective role in dopaminergic cell culture systems and could be of importance for the treatment of PD and other oxidative stress-related diseases. © 2014 Elsevier Ltd. All rights reserved.

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