No 1 Renming Hospital

Kunming, China

No 1 Renming Hospital

Kunming, China
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Yang J.,Yuxi Municipal Hospital of Traditional Chinese Medicine | Xu Z.-G.,Yuxi Municipal Hospital of Traditional Chinese Medicine | Rong R.,University of Sichuan | Lu Z.-P.,Yuxi Municipal Hospital of Traditional Chinese Medicine | And 6 more authors.
Journal of Sichuan University (Medical Science Edition) | Year: 2012

Objective To study the effect of methylprednisolone on spinal cord injury rats' neural behavior and the expression of brain derived neurotrophic factor (BDNF) and N-methyl-D-aspartic acid (NMDA). Methods To establish rat model of spinal cord injury (SCI), the rats were randomly divided into sham operation group, SCI group and methylprednisolone group (n=16 in each group). Eight rats were used for the behavioral assessment and BDNF measurement,the other eight animals was for the NMDA receptor test in each group. Within 8 h spinal cord contusion, methylprednisolone (50 mg/kg) was injected for methylprednisolone group, then after that the intramuscular injection of methylprednisolone was per day reduction in 10 mg/kg, till 5 days. By using immunohistochemical staining, the distribution of BDNF in the spinal cord and positive cell localization was observed and the number of positive cells were counted. The NMDA receptor affinity (Kd) and maximum binding amount (Bmax) were measured with [ 3H] MK-801 radioligand method, and the rat hind limb functional was also evaluated with BBB score analysis. Results Both the number of BDNF positive cells and the BBB score in methylprednisolone group was significant higher than that of SCI group; While increased receptor affinity (Kd) and decreased Bmax for NMDA receptor in methylprednisolone group was seen less than in SCI group ( P < 0.05).Conclusion Methylprednisolone Can Improve the Function of Rat Hind Limb, Increase BDNF Level and Decreased NMDA Receptor Expression after Spinal Cord Injury.


Xu Z.-G.,Yuxi Municipal Hospital of Traditional Chinese Medicine | Yang J.,Yuxi Municipal Hospital of Traditional Chinese Medicine | Lu Z.-P.,Yuxi Municipal Hospital of Traditional Chinese Medicine | Sun Y.-H.,Yuxi Municipal Hospital of Traditional Chinese Medicine | And 6 more authors.
Journal of Sichuan University (Medical Science Edition) | Year: 2012

Objective To study the effects of gold belt (GB), a Chinese Herbal, on behavioral changes and brain derived neutrophic factor (BDNF) expression and N-methyl-D-aspartic acid (NMDA) receptor level in rats subjected to spinal cord injury (SCI). Methods Adult male SD rats were randomly divided into three groups, 1 Sham group; 2 Spinal cord injury group (SCI group); 3 Spinal cord injury followed with gold belt treatment (gold belt 50 mg/(kg • d), intragastric gavage once daily for 7 days) group (GB group). The Basso, Beattie and Bresnahan (BBB) locomotor scale method was performed to evaluate the hindlimb motor function in the days 0,3, 10 and 28. After 13 days, 8 rats in each group were treated with 1% sodium pentobarbital (30 mg/kg), myoloid tissue in T10 position was taken and stored in liquid nitrogen to detect NMDA receptor affinity and maximum binding amount (Bmax) with radioligand binding assay. After 28 days, rats were sacrificed and the spinal cords were harvested for immunohistochemistry to observe the localization of BDNF in the ventral and dorsal horn of the spinal cord. Results After spinal cord contusion, GB resulted in a significant increase on the number of BDNF positive neurons compared with traumatic group, and increased BBB score and decreased NMDA receptor were also found in GB group. Whereas decreased BDNF expression, NMDA receptor affininty (Kd) were observed in traumatic injury group. Conclusion The gold belt treatment could effectively improve motor function, increase expression of BDNF, reduce the level of NMDA receptors in SCI rats. These data suggested that the gold belt played a role in the neuroplasticity after spinal cord injury.


Zhao N.,No 1 Renming Hospital | Dan Q.-Q.,University of Sichuan | Liu J.,No 1 Renming Hospital | Ma G.,No 1 Renming Hospital | And 4 more authors.
Journal of Sichuan University (Medical Science Edition) | Year: 2012

Objective To investigate the effect of singU neucleated cells transplantation on the neurological function and brain derived neurotrophic factor (BDNF) expression in traumatic brain injury (TBI) rats. Methods TBI rats were established by hammer fall method from 30 cm height with 50 g weight. Single neucleated cells/ karyocytes from bone marrow were separated and prepared by ficoll medium, then transplanted into pericontusional tissues in the brain. Neurological function severity scores (NSS) were recorded at 1, 3, and 7 days post operation (dpo). The pericontusional tissues were harvested at 7 dpo to analyze the BDNF localization, expressional level. Results Single neucleated cell transplantation decreased NSS significantly, compared with TBI rats without cell implantation (P<0.05). BDNF expression was upregulated and mainly found in neurons. Conclusion The present study showed the single karyocytes transplantation could improve neurological function and the mechanism is possibly linked to the BDNF expression.


PubMed | No 1 Renming Hospital
Type: Journal Article | Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition | Year: 2012

To investigate the effect of single neucleated cells transplantation on the neurological function and brain derived neurotrophic factor (BDNF) expression in traumatic brain injury (TBI) rats.TBI rats were established by hammer fall method from 30 cm height with 50 g weight. Single neucleated cells/ karyocytes from bone marrow were separated and prepared by ficoll medium, then transplanted into pericontusional tissues in the brain. Neurological function severity scores (NSS) were recorded at 1, 3, and 7 days post operation (dpo). The pericontusional tissues were harvested at 7 dpo to analyze the BDNF localization, expressional level.Single neucleated cell transplantation decreased NSS significantly, compared with TBI rats without cell implantation (P < 0.05). BDNF expression was upregulated and mainly found in neurons.The present study showed the single karyocytes transplantation could improve neurological function and the mechanism is possibly linked to the BDNF expression.

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