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Kim K.-Y.,NLP Co. | Ko M.-H.,Soonchunhyang University | Liu H.,CAS Wuhan Institute of Hydrobiology | Tang Q.,CAS Wuhan Institute of Hydrobiology | And 3 more authors.
BioMed Research International | Year: 2013

Gobionine species belonging to the genera Pseudorasbora, Pseudopungtungia, and Pungtungia (Teleostei; Cypriniformes; Cyprinidae) have been heavily studied because of problems on taxonomy, threats of extinction, invasion, and human health. Nucleotide sequences of three nuclear genes, that is, recombination activating protein gene 1 (rag1), recombination activating gene 2 (rag2), and early growth response 1 gene (egr1), from Pseudorasbora, Pseudopungtungia, and Pungtungia species residing in China, Japan, and Korea, were analyzed to elucidate their intergeneric and interspecific phylogenetic relationships. In the phylogenetic tree inferred from their multiple gene sequences, Pseudorasbora, Pseudopungtungia and Pungtungia species ramified into three phylogenetically distinct clades; the "tenuicorpa" clade composed of Pseudopungtungia tenuicorpa, the "parva" clade composed of all Pseudorasbora species/subspecies, and the "herzi" clade composed of Pseudopungtungia nigra, and Pungtungia herzi. The genus Pseudorasbora was recovered as monophyletic, while the genus Pseudopungtungia was recovered as polyphyletic. Our phylogenetic result implies the unstable taxonomic status of the genus Pseudopungtungia. © 2013 Keun-Yong Kim et al.


Jo W.S.,Korea Institute of Radiological and Medical Sciences | Choi Y.J.,Korea Institute of Radiological and Medical Sciences | Kim H.J.,Korea Institute of Radiological and Medical Sciences | Nam B.H.,Pusan National University | And 5 more authors.
Food Science and Biotechnology | Year: 2010

The aim of the present study was to examine the anti-inflammatory activities of extracts from Tetraselmis suecica with respect to nitric oxide (NO) production, tumor necrosis factor (TNF)-α and interlukin (IL)-6 release in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. We prepared methanolic extracts and water extract using protease. Of all the prepared extracts, 80% methanol extract exhibited the strongest anti-inflammatory effect. Thus, we further characterized the hexane fraction (He-TS) and ethyl acetate fraction (EA-TS) that contained potential bioactive compounds from the 80M-TS fraction of T. suecica. Both He-TS and EA-TS fractions resulted in a significant and dose-dependent inhibition of LPS-induced NO production, TNF-α, and IL-6 release (p<0.05). This strong inhibition of various inflammatory mediators such as NO, TNF-α, and IL-6 by EA-TS fraction from T. suecica suggests that this fraction might contain a potential bioactive compound that would be a good candidate for the development therapeutic anti inflammatory agent. © KoSFoST and Springer 2010.


Jo W.S.,Korea Institute of Radiological and Medical Sciences | Jo W.S.,NLP Co. | Yang K.M.,Korea Institute of Radiological and Medical Sciences | Yang K.M.,NLP Co. | And 9 more authors.
Toxicological Research | Year: 2012

Exposure of cells to ultraviolet B (UVB) radiation can induce production of free radicals and reactive oxygen species (ROS), which damage cellular components. In addition, these agents can stimulate the expression of matrix metalloproteinase (MMP) and decrease collagen synthesis in human skin cells. In this study, we examined the anti-photoaging effects of extracts of Tetraselmis suecica (W-TS). W-TS showed the strongest scavenging activity against 2,2-difenyl-1-picrylhydrazyl (DPPH) and peroxyl radicals, followed by superoxide anions from the xanthine/xanthine oxidase system. We observed that the levels of both intracellular ROS and lipid peroxidation significantly increased in UVB-irradiated human skin fibroblast cells. Furthermore, the activities of enzymatic antioxidants (e.g., superoxide dismutase) and the levels of non-enzymatic antioxidants (e.g., glutathione) significantly decreased in cells. However, W-TS pretreatment, at the maximum tested concentration, significantly decreased intracellular ROS and malondialdehyde (MDA) levels, and increased superoxide dismutase and glutathione levels in the cells. At this same concentration, W-TS did not show cytotoxicity. Type 1 procollagen and MMP-1 released were quantified using RT-PCR techniques. The results showed that W-TS protected type 1 procollagen against UVBinduced depletion in fibroblast cells in a dose-dependent manner via inhibition of UVB-induced MMP-1. Taken together, the results of the study suggest that W-TS effectively inhibits UVB-induced photoaging in skin fibroblasts by its strong anti-oxidant ability.


Park J.-Y.,NLP Corporation | Lee G.-A.,NLP Corporation | Kim K.-Y.,NLP Corporation | Choi S.-A.,Korea Institute of Energy Research | And 2 more authors.
Korean Chemical Engineering Research | Year: 2014

In this study, oil as a source of biodiesel from Nannochloropsis oceanica was extracted using organic solvent. The oil extraction yield and efficiency from dry and wet microalgae were investigated. The initial fatty acids content of the N. oceanica was 317.8 mg/g cell showing a high oil content over 30%. The yield from dry microalgae was higher than that from wet microalgae due to the inhibition of water. The yield by chloroform-methanol was the highest and the yield by hexane was the lowest. However, the total fatty acids contents with the chloroform-methanol were 678.7 and 778.2 mg/g oil under dry and wet conditions, respectively. The high oil extraction yield by chloroform-methanol reflected the fact that the extracted oil contained a high level of impurity. The hexane-methanol extraction from dry N. oceanica showed high oil extraction efficiency, 82.6%. The chloroform-methanol extraction under wet condition also showed high efficiency, 88.0%. While the hexane-methanol extraction from dry microalgae is desirable under low drying cost, the chloroform-methanol extraction from wet microalgae is desirable under high drying cost.


Choi S.-A.,Korea Institute of Energy Research | Choi S.-A.,Korea University | Choi W.-I.,Korea Institute of Energy Research | Lee J.-S.,Korea Institute of Energy Research | And 4 more authors.
Bioresource Technology | Year: 2015

In this study, hydrothermal acid treatment for efficient recovery of sugar from Golenkinia sp. was investigated. The initial glucose and XMG (xylose, mannose, and galactose) contents of a prepared Golenkinia sp. solution (40g/L) were 15.05 and 5.24g/L, respectively. The microalgal cell walls were hydrolyzed, for sugar recovery, by enzymatic saccharification and/or hydrothermal acid treatment. Among the various hydrothermal acid treatment conditions, the most optimal were the 2.0% H2SO4 concentration at 150°C for 15min, under which the glucose- and XMG-extraction yields were 71.7% and 64.9%, respectively. By pH 4.8, 50°C enzymatic hydrolysis after optimal hydrothermal acid treatment, the glucose- and XMG-extraction yields were additionally increased by 8.3% and 0.8%, respectively. After hydrothermal acid treatment, the combination with the enzymatic hydrolysis process improved the total sugar yield of Golenkinia sp. to 75.4%. © 2015 Elsevier Ltd.


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Nlp Llc | Date: 2014-12-19

downloadable mobile application for providing players of fantasy sports games with statistical and analytical tools based on aggregation of relevant sports data from the Internet and other sources.


Gobionine species belonging to the genera Pseudorasbora, Pseudopungtungia, and Pungtungia (Teleostei; Cypriniformes; Cyprinidae) have been heavily studied because of problems on taxonomy, threats of extinction, invasion, and human health. Nucleotide sequences of three nuclear genes, that is, recombination activating protein gene 1 (rag1), recombination activating gene 2 (rag2), and early growth response 1 gene (egr1), from Pseudorasbora, Pseudopungtungia, and Pungtungia species residing in China, Japan, and Korea, were analyzed to elucidate their intergeneric and interspecific phylogenetic relationships. In the phylogenetic tree inferred from their multiple gene sequences, Pseudorasbora, Pseudopungtungia and Pungtungia species ramified into three phylogenetically distinct clades; the tenuicorpa clade composed of Pseudopungtungia tenuicorpa, the parva clade composed of all Pseudorasbora species/subspecies, and the herzi clade composed of Pseudopungtungia nigra, and Pungtungia herzi. The genus Pseudorasbora was recovered as monophyletic, while the genus Pseudopungtungia was recovered as polyphyletic. Our phylogenetic result implies the unstable taxonomic status of the genus Pseudopungtungia.

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