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Nizhniy Novgorod, Russia

Nizhny Novgorod State Medical Academy, NNSMA is one of the medical schools in the Russian Federation which is located in the city of Nizhny Novgorod. Wikipedia.

Gordetsov A.S.,Nizhny Novgorod State Medical Academy
Sovremennye Tehnologii v Medicine

Now there is a great interest in experimental and clinical medicine to the new methods of diagnosis, treatment and prophylaxis of different diseases. It is particularly stipulated by appearance of multiple electronic devices, permitting to investigate the qualitative and quantitative characteristics of biological fluids and tissues at the nanotechnology level, making possible a revealing of the molecular transformation mechanisms, causing the pathologic changes in the human organism. The scientific works of the Nizhny Novgorod state medical academy collaborators and other scientists on investigation of the disease diagnosis new methods (including oncologic ones) on a basis of the biological material study by a method of infrared spectroscopy are analyzed. The question history is regarded. The methods of the biological fluid and tissue treatment and the IR-spectrum receipt are described; the examples of the IR-spectrum characteristic mathematical analysis are presented. A conclusion is made of the different disease treatment quality diagnosis and control new IR-spectroscopic method reliability. Source

Aghayeva U.F.,New York Academy of Sciences | Nikitin M.P.,RAS A.M. Prokhorov General Physics Institute | Lukash S.V.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Deyev S.M.,Nizhny Novgorod State Medical Academy
ACS Nano

To date, a number of biomolecule-mediated nanoparticle self-assembly systems have been developed that are amenable to controllable disassembly under relatively gentle conditions. However, for some applications such as design of self-assembled multifunctional theragnostic agents, high stability of the assembled structures can be of primary importance. Here, we report extraordinarily high durability of protein-assisted nanoparticle self-assembly systems yielding bifunctional colloidal superstructures resistant to extreme denaturing conditions intolerable for most proteins (e.g., high concentrations of chaotropic agents, high temperature). Among the tested systems (barnase-barstar (BBS), streptavidin-biotin, antibody-antigen, and protein A-immunoglobulin), the BBS is notable due to the combination of its high resistance to severe chemical perturbation and unique advantages offered by genetic engineering of this entirely protein-based system. Comparison of the self-assembly systems shows that whereas in all cases the preassembled structures proved essentially resistant to extreme conditions, the ability of the complementary biomolecular pairs to mediate assembly of the initial biomolecule-particle conjugates differs substantially in these conditions. © 2013 American Chemical Society. Source

Kleshnin M.S.,RAS Institute of Applied Physics | Turchin I.V.,Nizhny Novgorod State Medical Academy
Laser Physics Letters

The dispersion of biotissue optical properties results in distortion of the spectrum of radiation during propagation through biotissues. This phenomenon can be used for solving the inverse problem in fluorescence diffuse tomography. We have developed the spectrally resolved fluorescence diffuse tomography (SFDT) technique, which allows reconstruction of the spatial distribution of the fluorophore in biotissue even in the presence of unknown autofluorescence. The experimental setup combining epi-illumination and trans-illumination imaging geometries with spectral resolution has been created. Experimental studies on tissue phantoms and small animals in vivo with the proposed SFDT technique have been conducted. The obtained results have shown high accuracy of the inverse problem solution for spatial distribution of fluorophore concentration. © 2013 Astro Ltd. Source

Bugrova M.L.,Nizhny Novgorod State Medical Academy
Cell and Tissue Biology

We have studied the accumulation and excretion of atrial (ANP) and brain (BNP) natriuretic peptides in the early and late postreperfusion period (60 min and 60 days) in the myocardium of the right atrium in rats. The model of total ischemia proposed by Korpachev et al. (1982) was used. Immunocytochemical localization of peptides in cardiomyocytes was performed on ultrathin sections using the polyclonal antibodies. The intensities of accumulation (excretion) of ANP and BNP were analyzed by counting the immunolabeled granules (types A and B) with a transmission electron microscope. At 60 min and 60 days of the postreperfusion period, an increase in the synthesis and release of ANP and BNP was found. A more pronounced BNP reaction could be explained by the fact that, under normal conditions, the main hormone of the natriuretic peptide system regulating blood pressure is ANP, while BNP regulates blood pressure in cardiovascular pathology. © 2016, Pleiades Publishing, Ltd. Source

Selective coronary angiography may reveal slightly affected coronary arteries with less than 50% of stenotic areas in 10–15% of patients with clinical and ECG picture of acute coronary syndrome. The aim of the investigation is to evaluate the efficacy of the novel technique of endocardial and epicardial coronary bloodstream correction — intermittent percutaneous coronary sinus occlusion (PCSO) — in patients with acute coronary syndrome without hemodynamically significant atherosclerotic coronary lesions by studying the main functional parameters of the left ventricular myocardium in the medium terms (12 months later). Materials and Methods. The results of treating 8 patients with acute coronary syndrome and ST-segment depression without hemodynamically significant coronary stenoses and initial diastolic dysfunction have been analyzed. Four patients underwent PCSO with subsequent medication, other four patients received only therapeutic treatment. Results. In group 1 the myocardium function was observed to normalize completely in 2 individuals 12 months after the surgery (medium term). In group 2 complete normalization of diastolic myocardial function was not observed in any patient. Patients after PCSO showed statistically significantly better parameters of diastolic function 1 year after the operation compared to the results of only therapeutic treatment. Conclusion. PCSO procedure combined with subsequent adequate therapeutic support in patients with acute coronary syndrome has significantly better effect on restoration completeness of left ventricular diastolic function compared to isolated therapeutic treatment. © 2016, Nizhny Novgorod State Medical Academy. All rights reserved. Source

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